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Membrane bending occurs at all stages of clathrin-coat assembly and defines endocytic dynamics

Author

Listed:
  • Brandon L. Scott

    (RM 131 South Dakota State University (SDSU)
    SDSU)

  • Kem A. Sochacki

    (National Institutes of Health)

  • Shalini T. Low-Nam

    (RM 131 South Dakota State University (SDSU)
    SDSU
    University of California)

  • Elizabeth M. Bailey

    (RM 131 South Dakota State University (SDSU)
    SDSU)

  • QuocAhn Luu

    (South Dakota School of Mines and Technology (SDSMT)
    SDSMT)

  • Amy Hor

    (South Dakota School of Mines and Technology (SDSMT)
    SDSMT)

  • Andrea M. Dickey

    (National Institutes of Health)

  • Steve Smith

    (South Dakota School of Mines and Technology (SDSMT)
    SDSMT)

  • Jason G. Kerkvliet

    (RM 131 South Dakota State University (SDSU)
    SDSU)

  • Justin W. Taraska

    (National Institutes of Health)

  • Adam D. Hoppe

    (RM 131 South Dakota State University (SDSU)
    SDSU)

Abstract

Clathrin-mediated endocytosis (CME) internalizes plasma membrane by reshaping small regions of the cell surface into spherical vesicles. The key mechanistic question of how coat assembly produces membrane curvature has been studied with molecular and cellular structural biology approaches, without direct visualization of the process in living cells; resulting in two competing models for membrane bending. Here we use polarized total internal reflection fluorescence microscopy (pol-TIRF) combined with electron, atomic force, and super-resolution optical microscopy to measure membrane curvature during CME. Surprisingly, coat assembly accommodates membrane bending concurrent with or after the assembly of the clathrin lattice. Once curvature began, CME proceeded to scission with robust timing. Four color pol-TIRF showed that CALM accumulated at high levels during membrane bending, implicating its auxiliary role in curvature generation. We conclude that clathrin-coat assembly is versatile and that multiple membrane-bending trajectories likely reflect the energetics of coat assembly relative to competing forces.

Suggested Citation

  • Brandon L. Scott & Kem A. Sochacki & Shalini T. Low-Nam & Elizabeth M. Bailey & QuocAhn Luu & Amy Hor & Andrea M. Dickey & Steve Smith & Jason G. Kerkvliet & Justin W. Taraska & Adam D. Hoppe, 2018. "Membrane bending occurs at all stages of clathrin-coat assembly and defines endocytic dynamics," Nature Communications, Nature, vol. 9(1), pages 1-9, December.
  • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-02818-8
    DOI: 10.1038/s41467-018-02818-8
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    Cited by:

    1. Tomasz J. Nawara & Yancey D. Williams & Tejeshwar C. Rao & Yuesong Hu & Elizabeth Sztul & Khalid Salaita & Alexa L. Mattheyses, 2022. "Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    2. Changsong Yang & Patricia Colosi & Siewert Hugelier & Daniel Zabezhinsky & Melike Lakadamyali & Tatyana Svitkina, 2022. "Actin polymerization promotes invagination of flat clathrin-coated lattices in mammalian cells by pushing at lattice edges," Nature Communications, Nature, vol. 13(1), pages 1-20, December.
    3. Kazuki Obashi & Kem A. Sochacki & Marie-Paule Strub & Justin W. Taraska, 2023. "A conformational switch in clathrin light chain regulates lattice structure and endocytosis at the plasma membrane of mammalian cells," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

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