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The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells

Author

Listed:
  • Bijeta Prasai

    (National Heart, Lung, and Blood Institute, National Institutes of Health)

  • Gideon J. Haber

    (National Heart, Lung, and Blood Institute, National Institutes of Health)

  • Marie-Paule Strub

    (National Heart, Lung, and Blood Institute, National Institutes of Health)

  • Regina Ahn

    (National Heart, Lung, and Blood Institute, National Institutes of Health)

  • John A. Ciemniecki

    (National Heart, Lung, and Blood Institute, National Institutes of Health)

  • Kem A. Sochacki

    (National Heart, Lung, and Blood Institute, National Institutes of Health)

  • Justin W. Taraska

    (National Heart, Lung, and Blood Institute, National Institutes of Health)

Abstract

Rab-GTPases and their interacting partners are key regulators of secretory vesicle trafficking, docking, and fusion to the plasma membrane in neurons and neuroendocrine cells. Where and how these proteins are positioned and organized with respect to the vesicle and plasma membrane are unknown. Here, we use correlative super-resolution light and platinum replica electron microscopy to map Rab-GTPases (Rab27a and Rab3a) and their effectors (Granuphilin-a, Rabphilin3a, and Rim2) at the nanoscale in 2D. Next, we apply a targetable genetically-encoded electron microscopy labeling method that uses histidine based affinity-tags and metal-binding gold-nanoparticles to determine the 3D axial location of these exocytic proteins and two SNARE proteins (Syntaxin1A and SNAP25) using electron tomography. Rab proteins are distributed across the entire surface and t-SNARE proteins at the base of docked vesicles. We propose that the circumferential distribution of Rabs and Rab-effectors could aid in the efficient transport, capture, docking, and rapid fusion of calcium-triggered exocytic vesicles in excitable cells.

Suggested Citation

  • Bijeta Prasai & Gideon J. Haber & Marie-Paule Strub & Regina Ahn & John A. Ciemniecki & Kem A. Sochacki & Justin W. Taraska, 2021. "The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24167-9
    DOI: 10.1038/s41467-021-24167-9
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    Cited by:

    1. Kazuki Obashi & Kem A. Sochacki & Marie-Paule Strub & Justin W. Taraska, 2023. "A conformational switch in clathrin light chain regulates lattice structure and endocytosis at the plasma membrane of mammalian cells," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Fabian Lukas & Claudia Matthaeus & Tania López-Hernández & Ines Lahmann & Nicole Schultz & Martin Lehmann & Dmytro Puchkov & Jan Pielage & Volker Haucke & Tanja Maritzen, 2024. "Canonical and non-canonical integrin-based adhesions dynamically interconvert," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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