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Stabilization of membrane topologies by proteinaceous remorin scaffolds

Author

Listed:
  • Chao Su

    (University of Freiburg)

  • Marta Rodriguez-Franco

    (University of Freiburg)

  • Beatrice Lace

    (University of Freiburg)

  • Nils Nebel

    (University of Freiburg)

  • Casandra Hernandez-Reyes

    (University of Freiburg
    University of Freiburg)

  • Pengbo Liang

    (University of Freiburg)

  • Eija Schulze

    (University of Freiburg)

  • Evgeny V. Mymrikov

    (University of Freiburg
    University of Freiburg)

  • Nikolas M. Gross

    (University of Freiburg
    University of Freiburg
    University of Freiburg)

  • Julian Knerr

    (University of Freiburg)

  • Hong Wang

    (University of Freiburg
    University of Freiburg)

  • Lina Siukstaite

    (University of Freiburg
    University of Freiburg)

  • Jean Keller

    (Laboratoire de Recherche en Sciences Végétales (LRSV), Université de Toulouse, CNRS, UPS, INP Toulouse)

  • Cyril Libourel

    (Laboratoire de Recherche en Sciences Végétales (LRSV), Université de Toulouse, CNRS, UPS, INP Toulouse)

  • Alexandra A. M. Fischer

    (University of Freiburg
    University of Freiburg
    University of Freiburg
    University of Freiburg)

  • Katharina E. Gabor

    (University of Tübingen)

  • Eric Mark

    (University of Potsdam)

  • Claudia Popp

    (Ludwig-Maximilians-University (LMU) Munich, Institute of Genetics)

  • Carola Hunte

    (University of Freiburg
    University of Freiburg
    University of Freiburg)

  • Wilfried Weber

    (University of Freiburg
    University of Freiburg
    University of Freiburg)

  • Petra Wendler

    (University of Potsdam)

  • Thomas Stanislas

    (University of Tübingen)

  • Pierre-Marc Delaux

    (Laboratoire de Recherche en Sciences Végétales (LRSV), Université de Toulouse, CNRS, UPS, INP Toulouse)

  • Oliver Einsle

    (University of Freiburg)

  • Robert Grosse

    (University of Freiburg
    University of Freiburg)

  • Winfried Römer

    (University of Freiburg
    University of Freiburg
    University of Freiburg)

  • Thomas Ott

    (University of Freiburg
    University of Freiburg)

Abstract

In plants, the topological organization of membranes has mainly been attributed to the cell wall and the cytoskeleton. Additionally, few proteins, such as plant-specific remorins have been shown to function as protein and lipid organizers. Root nodule symbiosis requires continuous membrane re-arrangements, with bacteria being finally released from infection threads into membrane-confined symbiosomes. We found that mutations in the symbiosis-specific SYMREM1 gene result in highly disorganized perimicrobial membranes. AlphaFold modelling and biochemical analyses reveal that SYMREM1 oligomerizes into antiparallel dimers and may form a higher-order membrane scaffolding structure. This was experimentally confirmed when expressing this and other remorins in wall-less protoplasts is sufficient where they significantly alter and stabilize de novo membrane topologies ranging from membrane blebs to long membrane tubes with a central actin filament. Reciprocally, mechanically induced membrane indentations were equally stabilized by SYMREM1. Taken together we describe a plant-specific mechanism that allows the stabilization of large-scale membrane conformations independent of the cell wall.

Suggested Citation

  • Chao Su & Marta Rodriguez-Franco & Beatrice Lace & Nils Nebel & Casandra Hernandez-Reyes & Pengbo Liang & Eija Schulze & Evgeny V. Mymrikov & Nikolas M. Gross & Julian Knerr & Hong Wang & Lina Siuksta, 2023. "Stabilization of membrane topologies by proteinaceous remorin scaffolds," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-35976-5
    DOI: 10.1038/s41467-023-35976-5
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    References listed on IDEAS

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