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Receptor-mediated exopolysaccharide perception controls bacterial infection

Author

Listed:
  • Y. Kawaharada

    (Centre for Carbohydrate Recognition and Signalling. Aarhus University
    Aarhus University)

  • S. Kelly

    (Centre for Carbohydrate Recognition and Signalling. Aarhus University
    Aarhus University
    University of Otago)

  • M. Wibroe Nielsen

    (Centre for Carbohydrate Recognition and Signalling. Aarhus University
    Aarhus University)

  • C. T. Hjuler

    (Centre for Carbohydrate Recognition and Signalling. Aarhus University
    University of Copenhagen)

  • K. Gysel

    (Centre for Carbohydrate Recognition and Signalling. Aarhus University
    Aarhus University)

  • A. Muszyński

    (Complex Carbohydrate Research Center, University of Georgia)

  • R. W. Carlson

    (Complex Carbohydrate Research Center, University of Georgia)

  • M. B. Thygesen

    (Centre for Carbohydrate Recognition and Signalling. Aarhus University
    University of Copenhagen)

  • N. Sandal

    (Centre for Carbohydrate Recognition and Signalling. Aarhus University
    Aarhus University)

  • M. H. Asmussen

    (Centre for Carbohydrate Recognition and Signalling. Aarhus University
    Aarhus University)

  • M. Vinther

    (Centre for Carbohydrate Recognition and Signalling. Aarhus University
    Aarhus University)

  • S. U. Andersen

    (Centre for Carbohydrate Recognition and Signalling. Aarhus University
    Aarhus University)

  • L. Krusell

    (Centre for Carbohydrate Recognition and Signalling. Aarhus University
    Aarhus University)

  • S. Thirup

    (Centre for Carbohydrate Recognition and Signalling. Aarhus University
    Aarhus University)

  • K. J. Jensen

    (Centre for Carbohydrate Recognition and Signalling. Aarhus University
    University of Copenhagen)

  • C. W. Ronson

    (Centre for Carbohydrate Recognition and Signalling. Aarhus University
    University of Otago)

  • M. Blaise

    (Centre for Carbohydrate Recognition and Signalling. Aarhus University
    Aarhus University
    †Present address: Centre d’études d’agents Pathogènes et Biotechnologies pour la Santé, CNRS, Université de Montpellier, 34293 Montpellier, France)

  • S. Radutoiu

    (Centre for Carbohydrate Recognition and Signalling. Aarhus University
    Aarhus University)

  • J. Stougaard

    (Centre for Carbohydrate Recognition and Signalling. Aarhus University
    Aarhus University)

Abstract

Surface polysaccharides are important for bacterial interactions with multicellular organisms, and some are virulence factors in pathogens. In the legume–rhizobium symbiosis, bacterial exopolysaccharides (EPS) are essential for the development of infected root nodules. We have identified a gene in Lotus japonicus, Epr3, encoding a receptor-like kinase that controls this infection. We show that epr3 mutants are defective in perception of purified EPS, and that EPR3 binds EPS directly and distinguishes compatible and incompatible EPS in bacterial competition studies. Expression of Epr3 in epidermal cells within the susceptible root zone shows that the protein is involved in bacterial entry, while rhizobial and plant mutant studies suggest that Epr3 regulates bacterial passage through the plant’s epidermal cell layer. Finally, we show that Epr3 expression is inducible and dependent on host perception of bacterial nodulation (Nod) factors. Plant–bacterial compatibility and bacterial access to legume roots is thus regulated by a two-stage mechanism involving sequential receptor-mediated recognition of Nod factor and EPS signals.

Suggested Citation

  • Y. Kawaharada & S. Kelly & M. Wibroe Nielsen & C. T. Hjuler & K. Gysel & A. Muszyński & R. W. Carlson & M. B. Thygesen & N. Sandal & M. H. Asmussen & M. Vinther & S. U. Andersen & L. Krusell & S. Thir, 2015. "Receptor-mediated exopolysaccharide perception controls bacterial infection," Nature, Nature, vol. 523(7560), pages 308-312, July.
  • Handle: RePEc:nat:nature:v:523:y:2015:i:7560:d:10.1038_nature14611
    DOI: 10.1038/nature14611
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    Cited by:

    1. Giuseppe Malgioglio & Giulio Flavio Rizzo & Sebastian Nigro & Vincent Lefebvre du Prey & Joelle Herforth-Rahmé & Vittoria Catara & Ferdinando Branca, 2022. "Plant-Microbe Interaction in Sustainable Agriculture: The Factors That May Influence the Efficacy of PGPM Application," Sustainability, MDPI, vol. 14(4), pages 1-28, February.
    2. Chao Su & Marta Rodriguez-Franco & Beatrice Lace & Nils Nebel & Casandra Hernandez-Reyes & Pengbo Liang & Eija Schulze & Evgeny V. Mymrikov & Nikolas M. Gross & Julian Knerr & Hong Wang & Lina Siuksta, 2023. "Stabilization of membrane topologies by proteinaceous remorin scaffolds," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    3. Manuel Frank & Lavinia Ioana Fechete & Francesca Tedeschi & Marcin Nadzieja & Malita Malou Malekzadeh Nørgaard & Jesus Montiel & Kasper Røjkjær Andersen & Mikkel H. Schierup & Dugald Reid & Stig Ugger, 2023. "Single-cell analysis identifies genes facilitating rhizobium infection in Lotus japonicus," Nature Communications, Nature, vol. 14(1), pages 1-11, December.

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