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Cryo-electron tomography reveals structural insights into the membrane remodeling mode of dynamin-like EHD filaments

Author

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  • Arthur A. Melo

    (Structural Biology
    Institute of Chemistry and Biochemistry)

  • Thiemo Sprink

    (Structural Biology
    Charité - Universitätsmedizin Berlin at the MDC)

  • Jeffrey K. Noel

    (Structural Biology)

  • Elena Vázquez-Sarandeses

    (Structural Biology
    Institute of Chemistry and Biochemistry)

  • Chris Hoorn

    (Structural Biology)

  • Saif Mohd

    (Structural Biology
    Institute of Chemistry and Biochemistry)

  • Justus Loerke

    (Charité - Universitätsmedizin Berlin)

  • Christian M. T. Spahn

    (Charité - Universitätsmedizin Berlin)

  • Oliver Daumke

    (Structural Biology
    Institute of Chemistry and Biochemistry)

Abstract

Eps15-homology domain containing proteins (EHDs) are eukaryotic, dynamin-related ATPases involved in cellular membrane trafficking. They oligomerize on membranes into filaments that induce membrane tubulation. While EHD crystal structures in open and closed conformations were previously reported, little structural information is available for the membrane-bound oligomeric form. Consequently, mechanistic insights into the membrane remodeling mechanism have remained sparse. Here, by using cryo-electron tomography and subtomogram averaging, we determined structures of nucleotide-bound EHD4 filaments on membrane tubes of various diameters at an average resolution of 7.6 Å. Assembly of EHD4 is mediated via interfaces in the G-domain and the helical domain. The oligomerized EHD4 structure resembles the closed conformation, where the tips of the helical domains protrude into the membrane. The variation in filament geometry and tube radius suggests a spontaneous filament curvature of approximately 1/70 nm−1. Combining the available structural and functional data, we suggest a model for EHD-mediated membrane remodeling.

Suggested Citation

  • Arthur A. Melo & Thiemo Sprink & Jeffrey K. Noel & Elena Vázquez-Sarandeses & Chris Hoorn & Saif Mohd & Justus Loerke & Christian M. T. Spahn & Oliver Daumke, 2022. "Cryo-electron tomography reveals structural insights into the membrane remodeling mode of dynamin-like EHD filaments," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35164-x
    DOI: 10.1038/s41467-022-35164-x
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