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Lipid-mediated activation of plasma membrane-localized deubiquitylating enzymes modulate endosomal trafficking

Author

Listed:
  • Karin Vogel

    (University of Konstanz)

  • Tobias Bläske

    (University of Konstanz)

  • Marie-Kristin Nagel

    (University of Konstanz)

  • Christoph Globisch

    (University of Konstanz)

  • Shane Maguire

    (University of Konstanz)

  • Lorenz Mattes

    (University of Konstanz)

  • Christian Gude

    (Technical University of Munich)

  • Michael Kovermann

    (University of Konstanz)

  • Karin Hauser

    (University of Konstanz)

  • Christine Peter

    (University of Konstanz)

  • Erika Isono

    (University of Konstanz)

Abstract

The abundance of plasma membrane-resident receptors and transporters has to be tightly regulated by ubiquitin-mediated endosomal degradation for the proper coordination of environmental stimuli and intracellular signaling. Arabidopsis OVARIAN TUMOR PROTEASE (OTU) 11 and OTU12 are plasma membrane-localized deubiquitylating enzymes (DUBs) that bind to phospholipids through a polybasic motif in the OTU domain. Here we show that the DUB activity of OTU11 and OTU12 towards K63-linked ubiquitin is stimulated by binding to lipid membranes containing anionic lipids. In addition, we show that the DUB activity of OTU11 against K6- and K11-linkages is also stimulated by anionic lipids, and that OTU11 and OTU12 can modulate the endosomal degradation of a model cargo and the auxin efflux transporter PIN2-GFP in vivo. Our results suggest that the catalytic activity of OTU11 and OTU12 is tightly connected to their ability to bind membranes and that OTU11 and OTU12 are involved in the fine-tuning of plasma membrane proteins in Arabidopsis.

Suggested Citation

  • Karin Vogel & Tobias Bläske & Marie-Kristin Nagel & Christoph Globisch & Shane Maguire & Lorenz Mattes & Christian Gude & Michael Kovermann & Karin Hauser & Christine Peter & Erika Isono, 2022. "Lipid-mediated activation of plasma membrane-localized deubiquitylating enzymes modulate endosomal trafficking," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34637-3
    DOI: 10.1038/s41467-022-34637-3
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    1. Niccolò Mosesso & Niharika Savant Lerner & Tobias Bläske & Felix Groh & Shane Maguire & Marie Laura Niedermeier & Eliane Landwehr & Karin Vogel & Konstanze Meergans & Marie-Kristin Nagel & Malte Dresc, 2024. "Arabidopsis CaLB1 undergoes phase separation with the ESCRT protein ALIX and modulates autophagosome maturation," Nature Communications, Nature, vol. 15(1), pages 1-20, December.

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