IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms7151.html
   My bibliography  Save this article

Internalization and vacuolar targeting of the brassinosteroid hormone receptor BRI1 are regulated by ubiquitination

Author

Listed:
  • Sara Martins

    (Institute for Integrative Biology of the Cell (I2BC), CNRS, CEA, Paris-Sud University
    Institut des Sciences du Végétal, Unité Propre de Recherche 2355, Centre National de la Recherche Scientifique, Saclay Plant Sciences)

  • Esther M. N. Dohmann

    (University of Lausanne)

  • Anne Cayrel

    (Institute for Integrative Biology of the Cell (I2BC), CNRS, CEA, Paris-Sud University
    Institut des Sciences du Végétal, Unité Propre de Recherche 2355, Centre National de la Recherche Scientifique, Saclay Plant Sciences)

  • Alexander Johnson

    (Institute for Integrative Biology of the Cell (I2BC), CNRS, CEA, Paris-Sud University
    Institut des Sciences du Végétal, Unité Propre de Recherche 2355, Centre National de la Recherche Scientifique, Saclay Plant Sciences)

  • Wolfgang Fischer

    (The Salk Institute for Biological Studies)

  • Florence Pojer

    (Protein Crystallography Core Facility, Ecole Polytechnique Fédérale de Lausanne)

  • Béatrice Satiat-Jeunemaître

    (Institute for Integrative Biology of the Cell (I2BC), CNRS, CEA, Paris-Sud University
    Institut des Sciences du Végétal, Unité Propre de Recherche 2355, Centre National de la Recherche Scientifique, Saclay Plant Sciences)

  • Yvon Jaillais

    (Laboratoire de Reproduction et Développement des Plantes, INRA, CNRS, ENS Lyon, Université de Lyon)

  • Joanne Chory

    (The Salk Institute for Biological Studies
    Howard Hughes Medical Institute, The Salk Institute for Biological Studies)

  • Niko Geldner

    (University of Lausanne)

  • Grégory Vert

    (Institute for Integrative Biology of the Cell (I2BC), CNRS, CEA, Paris-Sud University
    Institut des Sciences du Végétal, Unité Propre de Recherche 2355, Centre National de la Recherche Scientifique, Saclay Plant Sciences)

Abstract

Brassinosteroids are plant steroid hormones that control many aspects of plant growth and development, and are perceived at the cell surface by the plasma membrane-localized receptor kinase BRI1. Here we show that BRI1 is post-translationally modified by K63 polyubiquitin chains in vivo. Using both artificial ubiquitination of BRI1 and generation of an ubiquitination-defective BRI1 mutant form, we demonstrate that ubiquitination promotes BRI1 internalization from the cell surface and is essential for its recognition at the trans-Golgi network/early endosomes (TGN/EE) for vacuolar targeting. Finally, we demonstrate that the control of BRI1 protein dynamics by ubiquitination is an important control mechanism for brassinosteroid responses in plants. Altogether, our results identify ubiquitination and K63-linked polyubiquitin chain formation as a dual targeting signal for BRI1 internalization and sorting along the endocytic pathway, and highlight its role in hormonally controlled plant development.

Suggested Citation

  • Sara Martins & Esther M. N. Dohmann & Anne Cayrel & Alexander Johnson & Wolfgang Fischer & Florence Pojer & Béatrice Satiat-Jeunemaître & Yvon Jaillais & Joanne Chory & Niko Geldner & Grégory Vert, 2015. "Internalization and vacuolar targeting of the brassinosteroid hormone receptor BRI1 are regulated by ubiquitination," Nature Communications, Nature, vol. 6(1), pages 1-11, May.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7151
    DOI: 10.1038/ncomms7151
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms7151
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms7151?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Sha Tang & Zhiying Zhao & Xiaotong Liu & Yi Sui & Dandan Zhang & Hui Zhi & Yuanzhu Gao & Hui Zhang & Linlin Zhang & Yannan Wang & Meicheng Zhao & Dongdong Li & Ke Wang & Qiang He & Renliang Zhang & We, 2023. "An E2-E3 pair contributes to seed size control in grain crops," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    2. Karin Vogel & Tobias Bläske & Marie-Kristin Nagel & Christoph Globisch & Shane Maguire & Lorenz Mattes & Christian Gude & Michael Kovermann & Karin Hauser & Christine Peter & Erika Isono, 2022. "Lipid-mediated activation of plasma membrane-localized deubiquitylating enzymes modulate endosomal trafficking," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    3. Hui Shi & Junjie Yin & Zhangjie Zhao & Hong Yu & Hong Yi & Li Xu & Huimin Tong & Min He & Xiaobo Zhu & Xiang Lu & Qing Xiong & Weitao Li & Yongyan Tang & Qingqing Hou & Li Song & Long Wang & Xiaoqiong, 2024. "Fine-tuning of IPA1 transactivation activity by E3 ligase IPI7-mediated non-proteolytic K29-ubiquitination during Magnaporthe oryzae infection," Nature Communications, Nature, vol. 15(1), pages 1-11, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7151. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.