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Vesicular release probability sets the strength of individual Schaffer collateral synapses

Author

Listed:
  • Céline D. Dürst

    (Center for Molecular Neurobiology Hamburg (ZMNH)
    University of Geneva)

  • J. Simon Wiegert

    (Center for Molecular Neurobiology Hamburg (ZMNH)
    Center for Molecular Neurobiology Hamburg (ZMNH))

  • Christian Schulze

    (Center for Molecular Neurobiology Hamburg (ZMNH))

  • Nordine Helassa

    (University of London
    University of Liverpool)

  • Katalin Török

    (University of London)

  • Thomas G. Oertner

    (Center for Molecular Neurobiology Hamburg (ZMNH))

Abstract

Information processing in the brain is controlled by quantal release of neurotransmitters, a tightly regulated process. From ultrastructural analysis, it is known that presynaptic boutons along single axons differ in the number of vesicles docked at the active zone. It is not clear whether the probability of these vesicles to get released (pves) is homogenous or also varies between individual boutons. Here, we optically measure evoked transmitter release at individual Schaffer collateral synapses at different calcium concentrations, using the genetically encoded glutamate sensor iGluSnFR. Fitting a binomial model to measured response amplitude distributions allowed us to extract the quantal parameters N, pves, and q. We find that Schaffer collateral boutons typically release single vesicles under low pves conditions and switch to multivesicular release in high calcium saline. The potency of individual boutons is highly correlated with their vesicular release probability while the number of releasable vesicles affects synaptic output only under high pves conditions.

Suggested Citation

  • Céline D. Dürst & J. Simon Wiegert & Christian Schulze & Nordine Helassa & Katalin Török & Thomas G. Oertner, 2022. "Vesicular release probability sets the strength of individual Schaffer collateral synapses," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33565-6
    DOI: 10.1038/s41467-022-33565-6
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    References listed on IDEAS

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    1. Ai-Hui Tang & Haiwen Chen & Tuo P. Li & Sarah R. Metzbower & Harold D. MacGillavry & Thomas A. Blanpied, 2016. "A trans-synaptic nanocolumn aligns neurotransmitter release to receptors," Nature, Nature, vol. 536(7615), pages 210-214, August.
    2. Zachary F. Mainen & Roberto Malinow & Karel Svoboda, 1999. "Synaptic calcium transients in single spines indicate that NMDA receptors are not saturated," Nature, Nature, vol. 399(6732), pages 151-155, May.
    3. Thomas P. Jensen & Kaiyu Zheng & Nicholas Cole & Jonathan S. Marvin & Loren L. Looger & Dmitri A. Rusakov, 2019. "Multiplex imaging relates quantal glutamate release to presynaptic Ca2+ homeostasis at multiple synapses in situ," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
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