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Sphingolipids mediate polar sorting of PIN2 through phosphoinositide consumption at the trans-Golgi network

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  • Yoko Ito

    (Laboratoire de Biogenèse Membranaire, Univ. Bordeaux)

  • Nicolas Esnay

    (Laboratoire de Biogenèse Membranaire, Univ. Bordeaux
    BioDiscovery Institute and Department of Biological Sciences, University of North Texas)

  • Matthieu Pierre Platre

    (Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon1, CNRS, INRAE
    Plant Molecular and Cellular Biology Laboratory and Integrative Biology Laboratory, Salk Institute for Biological Studies)

  • Valérie Wattelet-Boyer

    (Laboratoire de Biogenèse Membranaire, Univ. Bordeaux)

  • Lise C. Noack

    (Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon1, CNRS, INRAE)

  • Louise Fougère

    (Laboratoire de Biogenèse Membranaire, Univ. Bordeaux)

  • Wilhelm Menzel

    (Laboratoire de Biogenèse Membranaire, Univ. Bordeaux
    Leibniz Institute of Plant Biochemistry)

  • Stéphane Claverol

    (Platforme Proteome, Univ. Bordeaux)

  • Laetitia Fouillen

    (Laboratoire de Biogenèse Membranaire, Univ. Bordeaux
    MetaboHub-Bordeaux Metabolome INRAE)

  • Patrick Moreau

    (Laboratoire de Biogenèse Membranaire, Univ. Bordeaux
    Bordeaux Imaging Centre, Plant Imaging Platform, UMS 3420 University of Bordeaux-CNRS, INRAE)

  • Yvon Jaillais

    (Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon1, CNRS, INRAE)

  • Yohann Boutté

    (Laboratoire de Biogenèse Membranaire, Univ. Bordeaux)

Abstract

The lipid composition of organelles acts as a landmark to define membrane identity and specify subcellular function. Phosphoinositides are anionic lipids acting in protein sorting and trafficking at the trans-Golgi network (TGN). In animal cells, sphingolipids control the turnover of phosphoinositides through lipid exchange mechanisms at endoplasmic reticulum/TGN contact sites. In this study, we discover a mechanism for how sphingolipids mediate phosphoinositide homeostasis at the TGN in plant cells. Using multiple approaches, we show that a reduction of the acyl-chain length of sphingolipids results in an increased level of phosphatidylinositol-4-phosphate (PtdIns(4)P or PI4P) at the TGN but not of other lipids usually coupled to PI4P during exchange mechanisms. We show that sphingolipids mediate Phospholipase C (PLC)-driven consumption of PI4P at the TGN rather than local PI4P synthesis and that this mechanism is involved in the polar sorting of the auxin efflux carrier PIN2 at the TGN. Together, our data identify a mode of action of sphingolipids in lipid interplay at the TGN during protein sorting.

Suggested Citation

  • Yoko Ito & Nicolas Esnay & Matthieu Pierre Platre & Valérie Wattelet-Boyer & Lise C. Noack & Louise Fougère & Wilhelm Menzel & Stéphane Claverol & Laetitia Fouillen & Patrick Moreau & Yvon Jaillais & , 2021. "Sphingolipids mediate polar sorting of PIN2 through phosphoinositide consumption at the trans-Golgi network," Nature Communications, Nature, vol. 12(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24548-0
    DOI: 10.1038/s41467-021-24548-0
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    References listed on IDEAS

    as
    1. Joost C.M. Holthuis & Anant K. Menon, 2014. "Lipid landscapes and pipelines in membrane homeostasis," Nature, Nature, vol. 510(7503), pages 48-57, June.
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    Cited by:

    1. Rodrigo Enrique Gomez & Clément Chambaud & Josselin Lupette & Julie Castets & Stéphanie Pascal & Lysiane Brocard & Lise Noack & Yvon Jaillais & Jérôme Joubès & Amélie Bernard, 2022. "Phosphatidylinositol-4-phosphate controls autophagosome formation in Arabidopsis thaliana," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

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