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Nanoscale regulation of Ca2+ dependent phase transitions and real-time dynamics of SAP97/hDLG

Author

Listed:
  • Premchand Rajeev

    (Indian Institute of Science)

  • Nivedita Singh

    (Indian Institute of Science)

  • Adel Kechkar

    (Ecole Nationale Supérieure de Biotechnologie)

  • Corey Butler

    (Université Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297)

  • Narendrakumar Ramanan

    (Indian Institute of Science)

  • Jean-Baptiste Sibarita

    (Université Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297)

  • Mini Jose

    (Indian Institute of Science)

  • Deepak Nair

    (Indian Institute of Science)

Abstract

Synapse associated protein-97/Human Disk Large (SAP97/hDLG) is a conserved, alternatively spliced, modular, scaffolding protein critical in regulating the molecular organization of cell-cell junctions in vertebrates. We confirm that the molecular determinants of first order phase transition of SAP97/hDLG is controlled by morpho-functional changes in its nanoscale organization. Furthermore, the nanoscale molecular signatures of these signalling islands and phase transitions are altered in response to changes in cytosolic Ca2+. Additionally, exchange kinetics of alternatively spliced isoforms of the intrinsically disordered region in SAP97/hDLG C-terminus shows differential sensitivities to Ca2+ bound Calmodulin, affirming that the molecular signatures of local phase transitions of SAP97/hDLG depends on their nanoscale heterogeneity and compositionality of isoforms.

Suggested Citation

  • Premchand Rajeev & Nivedita Singh & Adel Kechkar & Corey Butler & Narendrakumar Ramanan & Jean-Baptiste Sibarita & Mini Jose & Deepak Nair, 2022. "Nanoscale regulation of Ca2+ dependent phase transitions and real-time dynamics of SAP97/hDLG," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31912-1
    DOI: 10.1038/s41467-022-31912-1
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    1. Pilong Li & Sudeep Banjade & Hui-Chun Cheng & Soyeon Kim & Baoyu Chen & Liang Guo & Marc Llaguno & Javoris V. Hollingsworth & David S. King & Salman F. Banani & Paul S. Russo & Qiu-Xing Jiang & B. Tra, 2012. "Phase transitions in the assembly of multivalent signalling proteins," Nature, Nature, vol. 483(7389), pages 336-340, March.
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    1. Manisha Poudyal & Komal Patel & Laxmikant Gadhe & Ajay Singh Sawner & Pradeep Kadu & Debalina Datta & Semanti Mukherjee & Soumik Ray & Ambuja Navalkar & Siddhartha Maiti & Debdeep Chatterjee & Jyoti D, 2023. "Intermolecular interactions underlie protein/peptide phase separation irrespective of sequence and structure at crowded milieu," Nature Communications, Nature, vol. 14(1), pages 1-21, December.

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