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Interchromosomal interaction of homologous Stat92E alleles regulates transcriptional switch during stem-cell differentiation

Author

Listed:
  • Matthew Antel

    (University of Connecticut Health Center)

  • Romir Raj

    (University of Connecticut Health Center)

  • Madona Y. G. Masoud

    (University of Connecticut Health Center)

  • Ziwei Pan

    (The Jackson Laboratory for Genomic Medicine
    University of Connecticut Health Center)

  • Sheng Li

    (The Jackson Laboratory for Genomic Medicine
    University of Connecticut Health Center)

  • Barbara G. Mellone

    (University of Connecticut
    University of Connecticut)

  • Mayu Inaba

    (University of Connecticut Health Center)

Abstract

Pairing of homologous chromosomes in somatic cells provides the opportunity of interchromosomal interaction between homologous gene regions. In the Drosophila male germline, the Stat92E gene is highly expressed in a germline stem cell (GSC) and gradually downregulated during the differentiation. Here we show that the pairing of Stat92E is always tight in GSCs and immediately loosened in differentiating daughter cells, gonialblasts (GBs). Disturbance of Stat92E pairing by relocation of one locus to another chromosome or by knockdown of global pairing/anti-pairing factors both result in a failure of Stat92E downregulation, suggesting that the pairing is required for the decline in transcription. Furthermore, the Stat92E enhancer, but not its transcription, is required for the change in pairing state, indicating that pairing is not a consequence of transcriptional changes. Finally, we show that the change in Stat92E pairing is dependent on asymmetric histone inheritance during the asymmetric division of GSCs. Taken together, we propose that the changes in Stat92E pairing status is an intrinsically programmed mechanism for enabling prompt cell fate switch during the differentiation of stem cells.

Suggested Citation

  • Matthew Antel & Romir Raj & Madona Y. G. Masoud & Ziwei Pan & Sheng Li & Barbara G. Mellone & Mayu Inaba, 2022. "Interchromosomal interaction of homologous Stat92E alleles regulates transcriptional switch during stem-cell differentiation," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31737-y
    DOI: 10.1038/s41467-022-31737-y
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    References listed on IDEAS

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