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A monomeric mycobacteriophage immunity repressor utilizes two domains to recognize an asymmetric DNA sequence

Author

Listed:
  • Reliza J. McGinnis

    (Western Carolina University, Department of Chemistry and Physics
    University of Michigan)

  • Chad A. Brambley

    (Middle Tennessee State University, Department of Chemistry)

  • Brandon Stamey

    (Western Carolina University, Department of Chemistry and Physics)

  • William C. Green

    (Western Carolina University, Department of Chemistry and Physics)

  • Kimberly N. Gragg

    (Western Carolina University, Department of Chemistry and Physics)

  • Erin R. Cafferty

    (Western Carolina University, Department of Chemistry and Physics
    University of Utah School of Medicine)

  • Thomas C. Terwilliger

    (New Mexico Consortium)

  • Michal Hammel

    (Lawrence Berkeley National Laboratory)

  • Thomas J. Hollis

    (Wake Forest University School of Medicine, Medical Center Boulevard)

  • Justin M. Miller

    (Middle Tennessee State University, Department of Chemistry)

  • Maria D. Gainey

    (Western Carolina University, Department of Chemistry and Physics)

  • Jamie R. Wallen

    (Western Carolina University, Department of Chemistry and Physics)

Abstract

Regulation of bacteriophage gene expression involves repressor proteins that bind and downregulate early lytic promoters. A large group of mycobacteriophages code for repressors that are unusual in also terminating transcription elongation at numerous binding sites (stoperators) distributed across the phage genome. Here we provide the X-ray crystal structure of a mycobacteriophage immunity repressor bound to DNA, which reveals the binding of a monomer to an asymmetric DNA sequence using two independent DNA binding domains. The structure is supported by small-angle X-ray scattering, DNA binding, molecular dynamics, and in vivo immunity assays. We propose a model for how dual DNA binding domains facilitate regulation of both transcription initiation and elongation, while enabling evolution of other superinfection immune specificities.

Suggested Citation

  • Reliza J. McGinnis & Chad A. Brambley & Brandon Stamey & William C. Green & Kimberly N. Gragg & Erin R. Cafferty & Thomas C. Terwilliger & Michal Hammel & Thomas J. Hollis & Justin M. Miller & Maria D, 2022. "A monomeric mycobacteriophage immunity repressor utilizes two domains to recognize an asymmetric DNA sequence," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31678-6
    DOI: 10.1038/s41467-022-31678-6
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    References listed on IDEAS

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