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Spatially resolved proteomic map shows that extracellular matrix regulates epidermal growth

Author

Listed:
  • Jun Li

    (Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Jie Ma

    (Beijing Institute of Lifeomics)

  • Qiyu Zhang

    (Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College
    Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Huizi Gong

    (Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College
    Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Dunqin Gao

    (Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Yujie Wang

    (Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Biyou Li

    (Beijing Institute of Lifeomics
    Anhui Medical University)

  • Xiao Li

    (Beijing Institute of Lifeomics)

  • Heyi Zheng

    (Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Zhihong Wu

    (Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Yunping Zhu

    (Beijing Institute of Lifeomics
    Anhui Medical University)

  • Ling Leng

    (Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College)

Abstract

Human skin comprises stratified squamous epithelium and dermis with various stromal cells and the extracellular matrix (ECM). The basement membrane (BM), a thin layer at the top of the dermis, serves as a unique niche for determining the fate of epidermal stem cells (EpSCs) by transmitting physical and biochemical signals to establish epidermal cell polarity and maintain the hierarchical structure and function of skin tissue. However, how stem cell niches maintain tissue homeostasis and control wound healing by regulating the behavior of EpSCs is still not completely understood. In this study, a hierarchical skin proteome map is constructed using spatial quantitative proteomics combined with decellularization, laser capture microdissection, and mass spectrometry. The specific functions of different structures of normal native skin tissues or tissues with a dermatologic disease are analyzed in situ. Transforming growth factor-beta (TGFβ)-induced protein ig-h3 (TGFBI), an ECM glycoprotein, in the BM is identified that could enhance the growth and function of EpSCs and promote wound healing. Our results provide insights into the way in which ECM proteins facilitate the growth and function of EpSCs as part of an important niche. The results may benefit the clinical treatment of skin ulcers or diseases with refractory lesions that involve epidermal cell dysfunction and re-epithelialization block in the future.

Suggested Citation

  • Jun Li & Jie Ma & Qiyu Zhang & Huizi Gong & Dunqin Gao & Yujie Wang & Biyou Li & Xiao Li & Heyi Zheng & Zhihong Wu & Yunping Zhu & Ling Leng, 2022. "Spatially resolved proteomic map shows that extracellular matrix regulates epidermal growth," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31659-9
    DOI: 10.1038/s41467-022-31659-9
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    1. Beatrice Dyring-Andersen & Marianne Bengtson Løvendorf & Fabian Coscia & Alberto Santos & Line Bruun Pilgaard Møller & Ana R. Colaço & Lili Niu & Michael Bzorek & Sophia Doll & Jørgen Lock Andersen & , 2020. "Spatially and cell-type resolved quantitative proteomic atlas of healthy human skin," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    2. Elaine Fuchs, 2007. "Scratching the surface of skin development," Nature, Nature, vol. 445(7130), pages 834-842, February.
    3. Aslamuzzaman Kazi & Shengyan Xiang & Hua Yang & Daniel Delitto & José Trevino & Rays H. Y. Jiang & Muhammad Ayaz & Harshani R. Lawrence & Perry Kennedy & Saïd M. Sebti, 2018. "GSK3 suppression upregulates β-catenin and c-Myc to abrogate KRas-dependent tumors," Nature Communications, Nature, vol. 9(1), pages 1-9, December.
    4. Shih-Min A. Huang & Yuji M. Mishina & Shanming Liu & Atwood Cheung & Frank Stegmeier & Gregory A. Michaud & Olga Charlat & Elizabeth Wiellette & Yue Zhang & Stephanie Wiessner & Marc Hild & Xiaoying S, 2009. "Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling," Nature, Nature, vol. 461(7264), pages 614-620, October.
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    1. Rui Chen & Jiasu Xu & Boqian Wang & Yi Ding & Aynur Abdulla & Yiyang Li & Lai Jiang & Xianting Ding, 2024. "SpiDe-Sr: blind super-resolution network for precise cell segmentation and clustering in spatial proteomics imaging," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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