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Spatially and cell-type resolved quantitative proteomic atlas of healthy human skin

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  • Beatrice Dyring-Andersen

    (Faculty of Health and Medical Sciences, University of Copenhagen
    Brigham and Women’s Hospital and Harvard Medical School
    University of Copenhagen
    Herlev and Gentofte Hospital, University of Copenhagen)

  • Marianne Bengtson Løvendorf

    (Aalborg University)

  • Fabian Coscia

    (Faculty of Health and Medical Sciences, University of Copenhagen)

  • Alberto Santos

    (Faculty of Health and Medical Sciences, University of Copenhagen)

  • Line Bruun Pilgaard Møller

    (Faculty of Health and Medical Sciences, University of Copenhagen)

  • Ana R. Colaço

    (Faculty of Health and Medical Sciences, University of Copenhagen)

  • Lili Niu

    (Faculty of Health and Medical Sciences, University of Copenhagen)

  • Michael Bzorek

    (Zealand University Hospital)

  • Sophia Doll

    (Max Planck Institute of Biochemistry)

  • Jørgen Lock Andersen

    (Zealand University Hospital)

  • Rachael A. Clark

    (Brigham and Women’s Hospital and Harvard Medical School)

  • Lone Skov

    (Herlev and Gentofte Hospital, University of Copenhagen)

  • Marcel B. M. Teunissen

    (Amsterdam University Medical Centers, location AMC)

  • Matthias Mann

    (Faculty of Health and Medical Sciences, University of Copenhagen
    Max Planck Institute of Biochemistry)

Abstract

Human skin provides both physical integrity and immunological protection from the external environment using functionally distinct layers, cell types and extracellular matrix. Despite its central role in human health and disease, the constituent proteins of skin have not been systematically characterized. Here, we combine advanced tissue dissection methods, flow cytometry and state-of-the-art proteomics to describe a spatially-resolved quantitative proteomic atlas of human skin. We quantify 10,701 proteins as a function of their spatial location and cellular origin. The resulting protein atlas and our initial data analyses demonstrate the value of proteomics for understanding cell-type diversity within the skin. We describe the quantitative distribution of structural proteins, known and previously undescribed proteins specific to cellular subsets and those with specialized immunological functions such as cytokines and chemokines. We anticipate that this proteomic atlas of human skin will become an essential community resource for basic and translational research ( https://skin.science/ ).

Suggested Citation

  • Beatrice Dyring-Andersen & Marianne Bengtson Løvendorf & Fabian Coscia & Alberto Santos & Line Bruun Pilgaard Møller & Ana R. Colaço & Lili Niu & Michael Bzorek & Sophia Doll & Jørgen Lock Andersen & , 2020. "Spatially and cell-type resolved quantitative proteomic atlas of healthy human skin," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19383-8
    DOI: 10.1038/s41467-020-19383-8
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    Cited by:

    1. Feng Wu & Yusheng Ren & Wenyan Lv & Xiaobing Liu & Xinyue Wang & Chuhan Wang & Zhenping Cao & Jinyao Liu & Jie Wei & Yan Pang, 2024. "Generating dual structurally and functionally skin-mimicking hydrogels by crosslinking cell-membrane compartments," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    2. Keitaro Fukuda & Yoshihiro Ito & Yuki Furuichi & Takeshi Matsui & Hiroto Horikawa & Takuya Miyano & Takaharu Okada & Mark Logtestijn & Reiko J. Tanaka & Atsushi Miyawaki & Masayuki Amagai, 2024. "Three stepwise pH progressions in stratum corneum for homeostatic maintenance of the skin," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    3. Jun Li & Jie Ma & Qiyu Zhang & Huizi Gong & Dunqin Gao & Yujie Wang & Biyou Li & Xiao Li & Heyi Zheng & Zhihong Wu & Yunping Zhu & Ling Leng, 2022. "Spatially resolved proteomic map shows that extracellular matrix regulates epidermal growth," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

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