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Homogeneous surrogate virus neutralization assay to rapidly assess neutralization activity of anti-SARS-CoV-2 antibodies

Author

Listed:
  • Sun Jin Kim

    (University of Utah)

  • Zhong Yao

    (University of Toronto)

  • Morgan C. Marsh

    (University of Utah)

  • Debra M. Eckert

    (University of Utah School of Medicine)

  • Michael S. Kay

    (University of Utah School of Medicine)

  • Anna Lyakisheva

    (University of Toronto)

  • Maria Pasic

    (University of Toronto
    St. Joseph’s Health Centre
    University of Toronto)

  • Aiyush Bansal

    (University of Toronto)

  • Chaim Birnboim

    (University of Toronto)

  • Prabhat Jha

    (University of Toronto)

  • Yannick Galipeau

    (University of Ottawa)

  • Marc-André Langlois

    (University of Ottawa
    University of Ottawa Centre for Infection, Immunity and Inflammation (CI3))

  • Julio C. Delgado

    (ARUP Institute for Clinical and Experimental Pathology
    University of Utah)

  • Marc G. Elgort

    (ARUP Institute for Clinical and Experimental Pathology)

  • Robert A. Campbell

    (University of Utah
    University of Utah
    University of Utah)

  • Elizabeth A. Middleton

    (University of Utah
    University of Utah
    University of Utah)

  • Igor Stagljar

    (University of Toronto
    University of Toronto
    University of Toronto
    Mediterranean Institute for Life Sciences)

  • Shawn C. Owen

    (University of Utah
    University of Utah
    University of Utah)

Abstract

The COVID-19 pandemic triggered the development of numerous diagnostic tools to monitor infection and to determine immune response. Although assays to measure binding antibodies against SARS-CoV-2 are widely available, more specific tests measuring neutralization activities of antibodies are immediately needed to quantify the extent and duration of protection that results from infection or vaccination. We previously developed a ‘Serological Assay based on a Tri-part split-NanoLuc® (SATiN)’ to detect antibodies that bind to the spike (S) protein of SARS-CoV-2. Here, we expand on our previous work and describe a reconfigured version of the SATiN assay, called Neutralization SATiN (Neu-SATiN), which measures neutralization activity of antibodies directly from convalescent or vaccinated sera. The results obtained with our assay and other neutralization assays are comparable but with significantly shorter preparation and run time for Neu-SATiN. As the assay is modular, we further demonstrate that Neu-SATiN enables rapid assessment of the effectiveness of vaccines and level of protection against existing SARS-CoV-2 variants of concern and can therefore be readily adapted for emerging variants.

Suggested Citation

  • Sun Jin Kim & Zhong Yao & Morgan C. Marsh & Debra M. Eckert & Michael S. Kay & Anna Lyakisheva & Maria Pasic & Aiyush Bansal & Chaim Birnboim & Prabhat Jha & Yannick Galipeau & Marc-André Langlois & J, 2022. "Homogeneous surrogate virus neutralization assay to rapidly assess neutralization activity of anti-SARS-CoV-2 antibodies," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31300-9
    DOI: 10.1038/s41467-022-31300-9
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    References listed on IDEAS

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    1. Antonio E. Muruato & Camila R. Fontes-Garfias & Ping Ren & Mariano A. Garcia-Blanco & Vineet D. Menachery & Xuping Xie & Pei-Yong Shi, 2020. "A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation," Nature Communications, Nature, vol. 11(1), pages 1-6, December.
    2. Lizhou Zhang & Cody B. Jackson & Huihui Mou & Amrita Ojha & Haiyong Peng & Brian D. Quinlan & Erumbi S. Rangarajan & Andi Pan & Abigail Vanderheiden & Mehul S. Suthar & Wenhui Li & Tina Izard & Christ, 2020. "SARS-CoV-2 spike-protein D614G mutation increases virion spike density and infectivity," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    3. Ugur Sahin & Alexander Muik & Evelyna Derhovanessian & Isabel Vogler & Lena M. Kranz & Mathias Vormehr & Alina Baum & Kristen Pascal & Jasmin Quandt & Daniel Maurus & Sebastian Brachtendorf & Verena L, 2020. "COVID-19 vaccine BNT162b1 elicits human antibody and TH1 T cell responses," Nature, Nature, vol. 586(7830), pages 594-599, October.
    4. Jun Lan & Jiwan Ge & Jinfang Yu & Sisi Shan & Huan Zhou & Shilong Fan & Qi Zhang & Xuanling Shi & Qisheng Wang & Linqi Zhang & Xinquan Wang, 2020. "Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor," Nature, Nature, vol. 581(7807), pages 215-220, May.
    5. Donal T. Skelly & Adam C. Harding & Javier Gilbert-Jaramillo & Michael L. Knight & Stephanie Longet & Anthony Brown & Sandra Adele & Emily Adland & Helen Brown & Tom Tipton & Lizzie Stafford & Alexand, 2021. "Two doses of SARS-CoV-2 vaccination induce robust immune responses to emerging SARS-CoV-2 variants of concern," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    6. Florian Krammer, 2020. "SARS-CoV-2 vaccines in development," Nature, Nature, vol. 586(7830), pages 516-527, October.
    7. Zhong Yao & Luka Drecun & Farzaneh Aboualizadeh & Sun Jin Kim & Zhijie Li & Heidi Wood & Emelissa J. Valcourt & Kathy Manguiat & Simon Plenderleith & Lily Yip & Xinliu Li & Zoe Zhong & Feng Yun Yue & , 2021. "A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    8. Donald J. Benton & Antoni G. Wrobel & Pengqi Xu & Chloë Roustan & Stephen R. Martin & Peter B. Rosenthal & John J. Skehel & Steven J. Gamblin, 2020. "Receptor binding and priming of the spike protein of SARS-CoV-2 for membrane fusion," Nature, Nature, vol. 588(7837), pages 327-330, December.
    9. Stefania Dispinseri & Massimiliano Secchi & Maria Franca Pirillo & Monica Tolazzi & Martina Borghi & Cristina Brigatti & Maria Laura Angelis & Marco Baratella & Elena Bazzigaluppi & Giulietta Venturi , 2021. "Neutralizing antibody responses to SARS-CoV-2 in symptomatic COVID-19 is persistent and critical for survival," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
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