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A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies

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Listed:
  • Zhong Yao

    (University of Toronto
    University of Toronto)

  • Luka Drecun

    (University of Toronto
    University of Toronto)

  • Farzaneh Aboualizadeh

    (University of Toronto
    University of Toronto)

  • Sun Jin Kim

    (University of Utah)

  • Zhijie Li

    (University of Toronto)

  • Heidi Wood

    (Zoonotic Diseases and Special Pathogens Division, National Microbiology Laboratory, Public Health Agency of Canada)

  • Emelissa J. Valcourt

    (Zoonotic Diseases and Special Pathogens Division, National Microbiology Laboratory, Public Health Agency of Canada)

  • Kathy Manguiat

    (Zoonotic Diseases and Special Pathogens Division, National Microbiology Laboratory, Public Health Agency of Canada)

  • Simon Plenderleith

    (Sunnybrook Research Institute)

  • Lily Yip

    (Sunnybrook Research Institute)

  • Xinliu Li

    (Department of Microbiology, Mount Sinai Hospital)

  • Zoe Zhong

    (Department of Microbiology, Mount Sinai Hospital)

  • Feng Yun Yue

    (University of Toronto)

  • Tatiana Closas

    (Canadian Blood Services)

  • Jamie Snider

    (University of Toronto
    University of Toronto)

  • Jelena Tomic

    (University of Toronto
    University of Toronto)

  • Steven J. Drews

    (Canadian Blood Services
    University of Alberta)

  • Michael A. Drebot

    (Zoonotic Diseases and Special Pathogens Division, National Microbiology Laboratory, Public Health Agency of Canada)

  • Allison McGeer

    (Department of Microbiology, Mount Sinai Hospital)

  • Mario Ostrowski

    (University of Toronto)

  • Samira Mubareka

    (Sunnybrook Research Institute
    University of Toronto)

  • James M. Rini

    (University of Toronto
    University of Toronto)

  • Shawn Owen

    (University of Utah
    University of Utah
    University of Utah)

  • Igor Stagljar

    (University of Toronto
    University of Toronto
    University of Toronto
    Mediterranean Institute for Life Sciences)

Abstract

Better diagnostic tools are needed to combat the ongoing COVID-19 pandemic. Here, to meet this urgent demand, we report a homogeneous immunoassay to detect IgG antibodies against SARS-CoV-2. This serological assay, called SATiN, is based on a tri-part Nanoluciferase (tNLuc) approach, in which the spike protein of SARS-CoV-2 and protein G, fused respectively to two different tNLuc tags, are used as antibody probes. Target engagement of the probes allows reconstitution of a functional luciferase in the presence of the third tNLuc component. The assay is performed directly in the liquid phase of patient sera and enables rapid, quantitative and low-cost detection. We show that SATiN has a similar sensitivity to ELISA, and its readouts are consistent with various neutralizing antibody assays. This proof-of-principle study suggests potential applications in diagnostics, as well as disease and vaccination management.

Suggested Citation

  • Zhong Yao & Luka Drecun & Farzaneh Aboualizadeh & Sun Jin Kim & Zhijie Li & Heidi Wood & Emelissa J. Valcourt & Kathy Manguiat & Simon Plenderleith & Lily Yip & Xinliu Li & Zoe Zhong & Feng Yun Yue & , 2021. "A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22102-6
    DOI: 10.1038/s41467-021-22102-6
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    Cited by:

    1. Yanan Tang & Turun Song & Lu Gao & Saifu Yin & Ming Ma & Yun Tan & Lijuan Wu & Yang Yang & Yanqun Wang & Tao Lin & Feng Li, 2022. "A CRISPR-based ultrasensitive assay detects attomolar concentrations of SARS-CoV-2 antibodies in clinical samples," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    2. Sun Jin Kim & Zhong Yao & Morgan C. Marsh & Debra M. Eckert & Michael S. Kay & Anna Lyakisheva & Maria Pasic & Aiyush Bansal & Chaim Birnboim & Prabhat Jha & Yannick Galipeau & Marc-André Langlois & J, 2022. "Homogeneous surrogate virus neutralization assay to rapidly assess neutralization activity of anti-SARS-CoV-2 antibodies," Nature Communications, Nature, vol. 13(1), pages 1-9, December.

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