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A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation

Author

Listed:
  • Antonio E. Muruato

    (University of Texas Medical Branch
    University of Texas Medical Branch)

  • Camila R. Fontes-Garfias

    (University of Texas Medical Branch)

  • Ping Ren

    (University of Texas Medical Branch)

  • Mariano A. Garcia-Blanco

    (University of Texas Medical Branch
    Programme in Emerging Infectious Diseases, Duke-NUS Medical School
    University of Texas Medical Branch)

  • Vineet D. Menachery

    (University of Texas Medical Branch
    University of Texas Medical Branch
    University of Texas Medical Branch)

  • Xuping Xie

    (University of Texas Medical Branch)

  • Pei-Yong Shi

    (University of Texas Medical Branch
    University of Texas Medical Branch
    University of Texas Medical Branch
    University of Texas Medical Branch)

Abstract

Virus neutralization remains the gold standard for determining antibody efficacy. Therefore, a high-throughput assay to measure SARS-CoV-2 neutralizing antibodies is urgently needed for COVID-19 serodiagnosis, convalescent plasma therapy, and vaccine development. Here, we report on a fluorescence-based SARS-CoV-2 neutralization assay that detects SARS-CoV-2 neutralizing antibodies in COVID-19 patient specimens and yields comparable results to plaque reduction neutralizing assay, the gold standard of serological testing. The fluorescence-based neutralization assay is specific to measure COVID-19 neutralizing antibodies without cross reacting with patient specimens with other viral, bacterial, or parasitic infections. Collectively, our approach offers a rapid platform that can be scaled to screen people for antibody protection from COVID-19, a key parameter necessary to safely reopen local communities.

Suggested Citation

  • Antonio E. Muruato & Camila R. Fontes-Garfias & Ping Ren & Mariano A. Garcia-Blanco & Vineet D. Menachery & Xuping Xie & Pei-Yong Shi, 2020. "A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation," Nature Communications, Nature, vol. 11(1), pages 1-6, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17892-0
    DOI: 10.1038/s41467-020-17892-0
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    Cited by:

    1. Sun Jin Kim & Zhong Yao & Morgan C. Marsh & Debra M. Eckert & Michael S. Kay & Anna Lyakisheva & Maria Pasic & Aiyush Bansal & Chaim Birnboim & Prabhat Jha & Yannick Galipeau & Marc-André Langlois & J, 2022. "Homogeneous surrogate virus neutralization assay to rapidly assess neutralization activity of anti-SARS-CoV-2 antibodies," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    2. Jing Zou & Hongjie Xia & Xuping Xie & Chaitanya Kurhade & Rafael R. G. Machado & Scott C. Weaver & Ping Ren & Pei-Yong Shi, 2022. "Neutralization against Omicron SARS-CoV-2 from previous non-Omicron infection," Nature Communications, Nature, vol. 13(1), pages 1-4, December.
    3. Yang Liu & Xianwen Zhang & Jianying Liu & Hongjie Xia & Jing Zou & Antonio E. Muruato & Sivakumar Periasamy & Chaitanya Kurhade & Jessica A. Plante & Nathen E. Bopp & Birte Kalveram & Alexander Bukrey, 2022. "A live-attenuated SARS-CoV-2 vaccine candidate with accessory protein deletions," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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