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A multifunctional system for genome editing and large-scale interspecies gene transfer

Author

Listed:
  • Marc Teufel

    (Philipps Universität Marburg, Synthetic Microbiology Center Marburg (SYNMIKRO))

  • Carlo A. Klein

    (Philipps Universität Marburg, Synthetic Microbiology Center Marburg (SYNMIKRO))

  • Maurice Mager

    (Philipps Universität Marburg, Synthetic Microbiology Center Marburg (SYNMIKRO))

  • Patrick Sobetzko

    (Philipps Universität Marburg, Synthetic Microbiology Center Marburg (SYNMIKRO))

Abstract

CRISPR SWAPnDROP extends the limits of genome editing to large-scale in-vivo DNA transfer between bacterial species. Its modular platform approach facilitates species specific adaptation to confer genome editing in various species. In this study, we show the implementation of the CRISPR SWAPnDROP concept for the model organism Escherichia coli, the fast growing Vibrio natriegens and the plant pathogen Dickeya dadantii. We demonstrate the excision, transfer and integration of large chromosomal regions between E. coli, V. natriegens and D. dadantii without size-limiting intermediate DNA extraction. CRISPR SWAPnDROP also provides common genome editing approaches comprising scarless, marker-free, iterative and parallel insertions and deletions. The modular character facilitates DNA library applications, and recycling of standardized parts. Its multi-color scarless co-selection system significantly improves editing efficiency and provides visual quality controls throughout the assembly and editing process.

Suggested Citation

  • Marc Teufel & Carlo A. Klein & Maurice Mager & Patrick Sobetzko, 2022. "A multifunctional system for genome editing and large-scale interspecies gene transfer," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30843-1
    DOI: 10.1038/s41467-022-30843-1
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    1. Harris H. Wang & Farren J. Isaacs & Peter A. Carr & Zachary Z. Sun & George Xu & Craig R. Forest & George M. Church, 2009. "Programming cells by multiplex genome engineering and accelerated evolution," Nature, Nature, vol. 460(7257), pages 894-898, August.
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