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A hexa-species transcriptome atlas of mammalian embryogenesis delineates metabolic regulation across three different implantation modes

Author

Listed:
  • Anna Malkowska

    (University of Cambridge
    Henry Wellcome Building of Cancer and Developmental Biology)

  • Christopher Penfold

    (University of Cambridge
    University of Cambridge
    University of Cambridge, Jeffrey Cheah Biomedical Centre)

  • Sophie Bergmann

    (University of Cambridge
    University of Cambridge
    University of Cambridge, Jeffrey Cheah Biomedical Centre)

  • Thorsten E. Boroviak

    (University of Cambridge
    University of Cambridge
    University of Cambridge, Jeffrey Cheah Biomedical Centre)

Abstract

Mammalian embryogenesis relies on glycolysis and oxidative phosphorylation to balance the generation of biomass with energy production. However, the dynamics of metabolic regulation in the postimplantation embryo in vivo have remained elusive due to the inaccessibility of the implanted conceptus for biochemical studies. To address this issue, we compiled single-cell embryo profiling data in six mammalian species and determined their metabolic dynamics through glycolysis and oxidative phosphorylation associated gene expression. Strikingly, we identify a conserved switch from bivalent respiration in the late blastocyst towards a glycolytic metabolism in early gastrulation stages across species, which is independent of embryo implantation. Extraembryonic lineages followed the dynamics of the embryonic lineage, except visceral endoderm. Finally, we demonstrate that in vitro primate embryo culture substantially impacts metabolic gene regulation by comparison to in vivo samples. Our work reveals a conserved metabolic programme despite different implantation modes and highlights the need to optimise postimplantation embryo culture protocols.

Suggested Citation

  • Anna Malkowska & Christopher Penfold & Sophie Bergmann & Thorsten E. Boroviak, 2022. "A hexa-species transcriptome atlas of mammalian embryogenesis delineates metabolic regulation across three different implantation modes," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30194-x
    DOI: 10.1038/s41467-022-30194-x
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