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Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis

Author

Listed:
  • Priscila Ramos-Ibeas

    (University of Nottingham
    National Institute for Agricultural and Food Research and Technology)

  • Fei Sang

    (University of Nottingham)

  • Qifan Zhu

    (University of Nottingham)

  • Walfred W. C. Tang

    (University of Cambridge
    University of Cambridge)

  • Sarah Withey

    (University of Nottingham
    Australian Institute for Bioengineering and Nanotechnology, University of Queensland)

  • Doris Klisch

    (University of Nottingham)

  • Liam Wood

    (University of Nottingham)

  • Matt Loose

    (University of Nottingham)

  • M. Azim Surani

    (University of Cambridge
    University of Cambridge
    University of Cambridge)

  • Ramiro Alberio

    (University of Nottingham)

Abstract

High-resolution molecular programmes delineating the cellular foundations of mammalian embryogenesis have emerged recently. Similar analysis of human embryos is limited to pre-implantation stages, since early post-implantation embryos are largely inaccessible. Notwithstanding, we previously suggested conserved principles of pig and human early development. For further insight on pluripotent states and lineage delineation, we analysed pig embryos at single cell resolution. Here we show progressive segregation of inner cell mass and trophectoderm in early blastocysts, and of epiblast and hypoblast in late blastocysts. We show that following an emergent short naive pluripotent signature in early embryos, there is a protracted appearance of a primed signature in advanced embryonic stages. Dosage compensation with respect to the X-chromosome in females is attained via X-inactivation in late epiblasts. Detailed human-pig comparison is a basis towards comprehending early human development and a foundation for further studies of human pluripotent stem cell differentiation in pig interspecies chimeras.

Suggested Citation

  • Priscila Ramos-Ibeas & Fei Sang & Qifan Zhu & Walfred W. C. Tang & Sarah Withey & Doris Klisch & Liam Wood & Matt Loose & M. Azim Surani & Ramiro Alberio, 2019. "Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis," Nature Communications, Nature, vol. 10(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08387-8
    DOI: 10.1038/s41467-019-08387-8
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    Cited by:

    1. Anna Malkowska & Christopher Penfold & Sophie Bergmann & Thorsten E. Boroviak, 2022. "A hexa-species transcriptome atlas of mammalian embryogenesis delineates metabolic regulation across three different implantation modes," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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