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Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae

Author

Listed:
  • Hui Liu

    (Jiangnan University
    Jiangnan University)

  • Pei Zhou

    (Jiangnan University
    Jiangnan University)

  • Mengya Qi

    (Jiangnan University
    Jiangnan University)

  • Liang Guo

    (Jiangnan University
    Jiangnan University)

  • Cong Gao

    (Jiangnan University
    Jiangnan University)

  • Guipeng Hu

    (Jiangnan University)

  • Wei Song

    (Jiangnan University)

  • Jing Wu

    (Jiangnan University)

  • Xiulai Chen

    (Jiangnan University
    Jiangnan University)

  • Jian Chen

    (Jiangnan University)

  • Wei Chen

    (Jiangnan University)

  • Liming Liu

    (Jiangnan University
    Jiangnan University)

Abstract

Saccharomyces cerevisiae is widely employed as a cell factory for the production of biofuels. However, product toxicity has hindered improvements in biofuel production. Here, we engineer the actin cytoskeleton in S. cerevisiae to increase both the cell growth and production of n-butanol and medium-chain fatty acids. Actin cable tortuosity is regulated using an n-butanol responsive promoter-based autonomous bidirectional signal conditioner in S. cerevisiae. The budding index is increased by 14.0%, resulting in the highest n-butanol titer of 1674.3 mg L−1. Moreover, actin patch density is fine-tuned using a medium-chain fatty acid responsive promoter-based autonomous bidirectional signal conditioner. The intracellular pH is stabilized at 6.4, yielding the highest medium-chain fatty acids titer of 692.3 mg L−1 in yeast extract peptone dextrose medium. Engineering the actin cytoskeleton in S. cerevisiae can efficiently alleviate biofuels toxicity and enhance biofuels production.

Suggested Citation

  • Hui Liu & Pei Zhou & Mengya Qi & Liang Guo & Cong Gao & Guipeng Hu & Wei Song & Jing Wu & Xiulai Chen & Jian Chen & Wei Chen & Liming Liu, 2022. "Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29560-6
    DOI: 10.1038/s41467-022-29560-6
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    References listed on IDEAS

    as
    1. Yueping Zhang & Juan Wang & Zibai Wang & Yiming Zhang & Shuobo Shi & Jens Nielsen & Zihe Liu, 2019. "A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    2. Qiang Ding & Danlei Ma & Gao-Qiang Liu & Yang Li & Liang Guo & Cong Gao & Guipeng Hu & Chao Ye & Jia Liu & Liming Liu & Xiulai Chen, 2020. "Author Correction: Light-powered Escherichia coli cell division for chemical production," Nature Communications, Nature, vol. 11(1), pages 1-1, December.
    3. Qiang Ding & Danlei Ma & Gao-Qiang Liu & Yang Li & Liang Guo & Cong Gao & Guipeng Hu & Chao Ye & Jia Liu & Liming Liu & Xiulai Chen, 2020. "Light-powered Escherichia coli cell division for chemical production," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    4. Miles W. Gander & Justin D. Vrana & William E. Voje & James M. Carothers & Eric Klavins, 2017. "Digital logic circuits in yeast with CRISPR-dCas9 NOR gates," Nature Communications, Nature, vol. 8(1), pages 1-11, August.
    5. I. V. Kukhtevich & N. Lohrberg & F. Padovani & R. Schneider & K. M. Schmoller, 2020. "Cell size sets the diameter of the budding yeast contractile ring," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
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