IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-29560-6.html
   My bibliography  Save this article

Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae

Author

Listed:
  • Hui Liu

    (Jiangnan University
    Jiangnan University)

  • Pei Zhou

    (Jiangnan University
    Jiangnan University)

  • Mengya Qi

    (Jiangnan University
    Jiangnan University)

  • Liang Guo

    (Jiangnan University
    Jiangnan University)

  • Cong Gao

    (Jiangnan University
    Jiangnan University)

  • Guipeng Hu

    (Jiangnan University)

  • Wei Song

    (Jiangnan University)

  • Jing Wu

    (Jiangnan University)

  • Xiulai Chen

    (Jiangnan University
    Jiangnan University)

  • Jian Chen

    (Jiangnan University)

  • Wei Chen

    (Jiangnan University)

  • Liming Liu

    (Jiangnan University
    Jiangnan University)

Abstract

Saccharomyces cerevisiae is widely employed as a cell factory for the production of biofuels. However, product toxicity has hindered improvements in biofuel production. Here, we engineer the actin cytoskeleton in S. cerevisiae to increase both the cell growth and production of n-butanol and medium-chain fatty acids. Actin cable tortuosity is regulated using an n-butanol responsive promoter-based autonomous bidirectional signal conditioner in S. cerevisiae. The budding index is increased by 14.0%, resulting in the highest n-butanol titer of 1674.3 mg L−1. Moreover, actin patch density is fine-tuned using a medium-chain fatty acid responsive promoter-based autonomous bidirectional signal conditioner. The intracellular pH is stabilized at 6.4, yielding the highest medium-chain fatty acids titer of 692.3 mg L−1 in yeast extract peptone dextrose medium. Engineering the actin cytoskeleton in S. cerevisiae can efficiently alleviate biofuels toxicity and enhance biofuels production.

Suggested Citation

  • Hui Liu & Pei Zhou & Mengya Qi & Liang Guo & Cong Gao & Guipeng Hu & Wei Song & Jing Wu & Xiulai Chen & Jian Chen & Wei Chen & Liming Liu, 2022. "Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29560-6
    DOI: 10.1038/s41467-022-29560-6
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-29560-6
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-29560-6?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Yueping Zhang & Juan Wang & Zibai Wang & Yiming Zhang & Shuobo Shi & Jens Nielsen & Zihe Liu, 2019. "A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    2. Qiang Ding & Danlei Ma & Gao-Qiang Liu & Yang Li & Liang Guo & Cong Gao & Guipeng Hu & Chao Ye & Jia Liu & Liming Liu & Xiulai Chen, 2020. "Author Correction: Light-powered Escherichia coli cell division for chemical production," Nature Communications, Nature, vol. 11(1), pages 1-1, December.
    3. Qiang Ding & Danlei Ma & Gao-Qiang Liu & Yang Li & Liang Guo & Cong Gao & Guipeng Hu & Chao Ye & Jia Liu & Liming Liu & Xiulai Chen, 2020. "Light-powered Escherichia coli cell division for chemical production," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    4. Miles W. Gander & Justin D. Vrana & William E. Voje & James M. Carothers & Eric Klavins, 2017. "Digital logic circuits in yeast with CRISPR-dCas9 NOR gates," Nature Communications, Nature, vol. 8(1), pages 1-11, August.
    5. I. V. Kukhtevich & N. Lohrberg & F. Padovani & R. Schneider & K. M. Schmoller, 2020. "Cell size sets the diameter of the budding yeast contractile ring," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Guangjian Li & Hui Liang & Ruichen Gao & Ling Qin & Pei Xu & Mingtao Huang & Min-Hua Zong & Yufei Cao & Wen-Yong Lou, 2024. "Yeast metabolism adaptation for efficient terpenoids synthesis via isopentenol utilization," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Wentao Zheng & Yuxuan Wang & Jie Cui & Guangyao Guo & Yufeng Li & Jin Hou & Qiang Tu & Yulong Yin & Francis Stewart & Youming Zhang & Xiaoying Bian & Xue Wang, 2024. "ReaL-MGE is a tool for enhanced multiplex genome engineering and application to malonyl-CoA anabolism," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    2. Liu, Zihe & Moradi, Hamideh & Shi, Shuobo & Darvishi, Farshad, 2021. "Yeasts as microbial cell factories for sustainable production of biofuels," Renewable and Sustainable Energy Reviews, Elsevier, vol. 143(C).
    3. William M. Shaw & Lucie Studená & Kyler Roy & Piotr Hapeta & Nicholas S. McCarty & Alicia E. Graham & Tom Ellis & Rodrigo Ledesma-Amaro, 2022. "Inducible expression of large gRNA arrays for multiplexed CRISPRai applications," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    4. Brian D. Huang & Dowan Kim & Yongjoon Yu & Corey J. Wilson, 2024. "Engineering intelligent chassis cells via recombinase-based MEMORY circuits," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    5. Wang, Kai & Da, Yangyang & Bi, Haoran & Liu, Yanhui & Chen, Biqiang & Wang, Meng & Liu, Zihe & Nielsen, Jens & Tan, Tianwei, 2023. "A one-carbon chemicals conversion strategy to produce precursor of biofuels with Saccharomyces cerevisiae," Renewable Energy, Elsevier, vol. 208(C), pages 331-340.
    6. Dixit, Yatika & Yadav, Preeti & Sharma, Arun Kumar & Pandey, Poornima & Kuila, Arindam, 2023. "Multiplex genome editing to construct cellulase engineered Saccharomyces cerevisiae for ethanol production from cellulosic biomass," Renewable and Sustainable Energy Reviews, Elsevier, vol. 187(C).
    7. Yuanli Gao & Lei Wang & Baojun Wang, 2023. "Customizing cellular signal processing by synthetic multi-level regulatory circuits," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    8. Qun Yue & Jie Meng & Yue Qiu & Miaomiao Yin & Liwen Zhang & Weiping Zhou & Zhiqiang An & Zihe Liu & Qipeng Yuan & Wentao Sun & Chun Li & Huimin Zhao & István Molnár & Yuquan Xu & Shuobo Shi, 2023. "A polycistronic system for multiplexed and precalibrated expression of multigene pathways in fungi," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    9. Baiyang Liu & Christian Cuba Samaniego & Matthew R. Bennett & Elisa Franco & James Chappell, 2023. "A portable regulatory RNA array design enables tunable and complex regulation across diverse bacteria," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    10. Luna Rizik & Loai Danial & Mouna Habib & Ron Weiss & Ramez Daniel, 2022. "Synthetic neuromorphic computing in living cells," Nature Communications, Nature, vol. 13(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29560-6. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.