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Light-powered Escherichia coli cell division for chemical production

Author

Listed:
  • Qiang Ding

    (Jiangnan University
    Jiangnan University)

  • Danlei Ma

    (Jiangnan University
    Jiangnan University)

  • Gao-Qiang Liu

    (Central South University of Forestry and Technology)

  • Yang Li

    (Jiangnan University
    Jiangnan University)

  • Liang Guo

    (Jiangnan University
    Jiangnan University)

  • Cong Gao

    (Jiangnan University
    Jiangnan University)

  • Guipeng Hu

    (Jiangnan University
    Jiangnan University)

  • Chao Ye

    (Jiangnan University
    Jiangnan University)

  • Jia Liu

    (Jiangnan University
    Jiangnan University)

  • Liming Liu

    (Jiangnan University
    Jiangnan University
    Jiangnan University)

  • Xiulai Chen

    (Jiangnan University
    Jiangnan University)

Abstract

Cell division can perturb the metabolic performance of industrial microbes. The C period of cell division starts from the initiation to the termination of DNA replication, whereas the D period is the bacterial division process. Here, we first shorten the C and D periods of E. coli by controlling the expression of the ribonucleotide reductase NrdAB and division proteins FtsZA through blue light and near-infrared light activation, respectively. It increases the specific surface area to 3.7 μm−1 and acetoin titer to 67.2 g·L−1. Next, we prolong the C and D periods of E. coli by regulating the expression of the ribonucleotide reductase NrdA and division protein inhibitor SulA through blue light activation-repression and near-infrared (NIR) light activation, respectively. It improves the cell volume to 52.6 μm3 and poly(lactate-co-3-hydroxybutyrate) titer to 14.31 g·L−1. Thus, the optogenetic-based cell division regulation strategy can improve the efficiency of microbial cell factories.

Suggested Citation

  • Qiang Ding & Danlei Ma & Gao-Qiang Liu & Yang Li & Liang Guo & Cong Gao & Guipeng Hu & Chao Ye & Jia Liu & Liming Liu & Xiulai Chen, 2020. "Light-powered Escherichia coli cell division for chemical production," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16154-3
    DOI: 10.1038/s41467-020-16154-3
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    Cited by:

    1. Wang, Kai & Da, Yangyang & Bi, Haoran & Liu, Yanhui & Chen, Biqiang & Wang, Meng & Liu, Zihe & Nielsen, Jens & Tan, Tianwei, 2023. "A one-carbon chemicals conversion strategy to produce precursor of biofuels with Saccharomyces cerevisiae," Renewable Energy, Elsevier, vol. 208(C), pages 331-340.
    2. Hui Liu & Pei Zhou & Mengya Qi & Liang Guo & Cong Gao & Guipeng Hu & Wei Song & Jing Wu & Xiulai Chen & Jian Chen & Wei Chen & Liming Liu, 2022. "Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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