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Small RNA pathways in the nematode Ascaris in the absence of piRNAs

Author

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  • Maxim V. Zagoskin

    (University of Colorado School of Medicine
    University of Colorado School of Medicine
    University of Tennessee)

  • Jianbin Wang

    (University of Colorado School of Medicine
    University of Colorado School of Medicine
    University of Tennessee
    University of Tennessee)

  • Ashley T. Neff

    (University of Colorado School of Medicine)

  • Giovana M. B. Veronezi

    (University of Colorado School of Medicine)

  • Richard E. Davis

    (University of Colorado School of Medicine
    University of Colorado School of Medicine)

Abstract

Small RNA pathways play key and diverse regulatory roles in C. elegans, but our understanding of their conservation and contributions in other nematodes is limited. We analyzed small RNA pathways in the divergent parasitic nematode Ascaris. Ascaris has ten Argonautes with five worm-specific Argonautes (WAGOs) that associate with secondary 5’-triphosphate 22-24G-RNAs. These small RNAs target repetitive sequences or mature mRNAs and are similar to the C. elegans mutator, nuclear, and CSR-1 small RNA pathways. Even in the absence of a piRNA pathway, Ascaris CSR-1 may still function to “license” as well as fine-tune or repress gene expression. Ascaris ALG-4 and its associated 26G-RNAs target and likely repress specific mRNAs during testis meiosis. Ascaris WAGO small RNAs demonstrate target plasticity changing their targets between repeats and mRNAs during development. We provide a unique and comprehensive view of mRNA and small RNA expression throughout spermatogenesis. Overall, our study illustrates the conservation, divergence, dynamics, and flexibility of small RNA pathways in nematodes.

Suggested Citation

  • Maxim V. Zagoskin & Jianbin Wang & Ashley T. Neff & Giovana M. B. Veronezi & Richard E. Davis, 2022. "Small RNA pathways in the nematode Ascaris in the absence of piRNAs," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28482-7
    DOI: 10.1038/s41467-022-28482-7
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