IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-26508-0.html
   My bibliography  Save this article

Structural basis of terephthalate recognition by solute binding protein TphC

Author

Listed:
  • Trishnamoni Gautom

    (The University of Manchester
    Gauhati University
    Royal Global University)

  • Dharmendra Dheeman

    (The University of Manchester)

  • Colin Levy

    (The University of Manchester)

  • Thomas Butterfield

    (The University of Manchester)

  • Guadalupe Alvarez Gonzalez

    (The University of Manchester)

  • Philip Roy

    (The University of Manchester)

  • Lewis Caiger

    (The University of Manchester)

  • Karl Fisher

    (The University of Manchester)

  • Linus Johannissen

    (The University of Manchester)

  • Neil Dixon

    (The University of Manchester)

Abstract

Biological degradation of Polyethylene terephthalate (PET) plastic and assimilation of the corresponding monomers ethylene glycol and terephthalate (TPA) into central metabolism offers an attractive route for bio-based molecular recycling and bioremediation applications. A key step is the cellular uptake of the non-permeable TPA into bacterial cells which has been shown to be dependent upon the presence of the key tphC gene. However, little is known from a biochemical and structural perspective about the encoded solute binding protein, TphC. Here, we report the biochemical and structural characterisation of TphC in both open and TPA-bound closed conformations. This analysis demonstrates the narrow ligand specificity of TphC towards aromatic para-substituted dicarboxylates, such as TPA and closely related analogues. Further phylogenetic and genomic context analysis of the tph genes reveals homologous operons as a genetic resource for future biotechnological and metabolic engineering efforts towards circular plastic bio-economy solutions.

Suggested Citation

  • Trishnamoni Gautom & Dharmendra Dheeman & Colin Levy & Thomas Butterfield & Guadalupe Alvarez Gonzalez & Philip Roy & Lewis Caiger & Karl Fisher & Linus Johannissen & Neil Dixon, 2021. "Structural basis of terephthalate recognition by solute binding protein TphC," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26508-0
    DOI: 10.1038/s41467-021-26508-0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-26508-0
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-26508-0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Seongjoon Joo & In Jin Cho & Hogyun Seo & Hyeoncheol Francis Son & Hye-Young Sagong & Tae Joo Shin & So Young Choi & Sang Yup Lee & Kyung-Jin Kim, 2018. "Structural insight into molecular mechanism of poly(ethylene terephthalate) degradation," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    2. V. Tournier & C. M. Topham & A. Gilles & B. David & C. Folgoas & E. Moya-Leclair & E. Kamionka & M.-L. Desrousseaux & H. Texier & S. Gavalda & M. Cot & E. Guémard & M. Dalibey & J. Nomme & G. Cioci & , 2020. "An engineered PET depolymerase to break down and recycle plastic bottles," Nature, Nature, vol. 580(7802), pages 216-219, April.
    3. Xu Han & Weidong Liu & Jian-Wen Huang & Jiantao Ma & Yingying Zheng & Tzu-Ping Ko & Limin Xu & Ya-Shan Cheng & Chun-Chi Chen & Rey-Ting Guo, 2017. "Structural insight into catalytic mechanism of PET hydrolase," Nature Communications, Nature, vol. 8(1), pages 1-6, December.
    4. Gottfried J. Palm & Lukas Reisky & Dominique Böttcher & Henrik Müller & Emil A. P. Michels & Miriam C. Walczak & Leona Berndt & Manfred S. Weiss & Uwe T. Bornscheuer & Gert Weber, 2019. "Structure of the plastic-degrading Ideonella sakaiensis MHETase bound to a substrate," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Zhuozhi Chen & Rongdi Duan & Yunjie Xiao & Yi Wei & Hanxiao Zhang & Xinzhao Sun & Shen Wang & Yingying Cheng & Xue Wang & Shanwei Tong & Yunxiao Yao & Cheng Zhu & Haitao Yang & Yanyan Wang & Zefang Wa, 2022. "Biodegradation of highly crystallized poly(ethylene terephthalate) through cell surface codisplay of bacterial PETase and hydrophobin," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    2. Erika Erickson & Japheth E. Gado & Luisana Avilán & Felicia Bratti & Richard K. Brizendine & Paul A. Cox & Raj Gill & Rosie Graham & Dong-Jin Kim & Gerhard König & William E. Michener & Saroj Poudel &, 2022. "Sourcing thermotolerant poly(ethylene terephthalate) hydrolase scaffolds from natural diversity," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    3. P. Konstantin Richter & Paula Blázquez-Sánchez & Ziyue Zhao & Felipe Engelberger & Christian Wiebeler & Georg Künze & Ronny Frank & Dana Krinke & Emanuele Frezzotti & Yuliia Lihanova & Patricia Falken, 2023. "Structure and function of the metagenomic plastic-degrading polyester hydrolase PHL7 bound to its product," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    4. Hwaseok Hong & Dongwoo Ki & Hogyun Seo & Jiyoung Park & Jaewon Jang & Kyung-Jin Kim, 2023. "Discovery and rational engineering of PET hydrolase with both mesophilic and thermophilic PET hydrolase properties," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    5. Anni Li & Yijie Sheng & Haiyang Cui & Minghui Wang & Luxuan Wu & Yibo Song & Rongrong Yang & Xiujuan Li & He Huang, 2023. "Discovery and mechanism-guided engineering of BHET hydrolases for improved PET recycling and upcycling," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    6. Yu Yang & Jian Min & Ting Xue & Pengcheng Jiang & Xin Liu & Rouming Peng & Jian-Wen Huang & Yingying Qu & Xian Li & Ning Ma & Fang-Chang Tsai & Longhai Dai & Qi Zhang & Yingle Liu & Chun-Chi Chen & Re, 2023. "Complete bio-degradation of poly(butylene adipate-co-terephthalate) via engineered cutinases," Nature Communications, Nature, vol. 14(1), pages 1-8, December.
    7. Teng Bao & Yuanchao Qian & Yongping Xin & James J. Collins & Ting Lu, 2023. "Engineering microbial division of labor for plastic upcycling," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    8. Katarzyna Świderek & Susana Velasco-Lozano & Miquel À. Galmés & Ion Olazabal & Haritz Sardon & Fernando López-Gallego & Vicent Moliner, 2023. "Mechanistic studies of a lipase unveil effect of pH on hydrolysis products of small PET modules," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    9. Huang, Jijiang & Veksha, Andrei & Chan, Wei Ping & Giannis, Apostolos & Lisak, Grzegorz, 2022. "Chemical recycling of plastic waste for sustainable material management: A prospective review on catalysts and processes," Renewable and Sustainable Energy Reviews, Elsevier, vol. 154(C).
    10. Gui Zhao & Jiayi Lin & Mengying Lu & Lina Li & Pengtao Xu & Xi Liu & Liwei Chen, 2024. "Potential cycling boosts the electrochemical conversion of polyethylene terephthalate-derived alcohol into valuable chemicals," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    11. Yuantao Peng & Jie Yang & Chenqiang Deng & Jin Deng & Li Shen & Yao Fu, 2023. "Acetolysis of waste polyethylene terephthalate for upcycling and life-cycle assessment study," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    12. Iman Ibrahim & Ayat Gamal Ashour & Waleed Zeiada & Nisreen Salem & Mohamed Abdallah, 2024. "A Systematic Review on the Technical Performance and Sustainability of 3D Printing Filaments Using Recycled Plastic," Sustainability, MDPI, vol. 16(18), pages 1-32, September.
    13. Jingjing Cao & Huaxing Liang & Jie Yang & Zhiyang Zhu & Jin Deng & Xiaodong Li & Menachem Elimelech & Xinglin Lu, 2024. "Depolymerization mechanisms and closed-loop assessment in polyester waste recycling," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    14. Jun Qi & Yadong Du & Qi Yang & Na Jiang & Jiachun Li & Yi Ma & Yangjun Ma & Xin Zhao & Jieshan Qiu, 2023. "Energy-saving and product-oriented hydrogen peroxide electrosynthesis enabled by electrochemistry pairing and product engineering," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    15. Elizabeth L. Bell & Gloria Rosetto & Morgan A. Ingraham & Kelsey J. Ramirez & Clarissa Lincoln & Ryan W. Clarke & Japheth E. Gado & Jacob L. Lilly & Katarzyna H. Kucharzyk & Erika Erickson & Gregg T. , 2024. "Natural diversity screening, assay development, and characterization of nylon-6 enzymatic depolymerization," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    16. Javier M. Hernández-Sancho & Arnaud Boudigou & Maria V. G. Alván-Vargas & Dekel Freund & Jenny Arnling Bååth & Peter Westh & Kenneth Jensen & Lianet Noda-García & Daniel C. Volke & Pablo I. Nikel, 2024. "A versatile microbial platform as a tunable whole-cell chemical sensor," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    17. Gluth, A. & Xu, Z. & Fifield, L.S. & Yang, B., 2022. "Advancing biological processing for valorization of plastic wastes," Renewable and Sustainable Energy Reviews, Elsevier, vol. 170(C).
    18. Kamali, Ali Reza & Li, Siyuan, 2023. "Molten salt-assisted valorization of waste PET plastics into nanostructured SnO2@terephthalic acid with excellent Li-ion storage performance," Applied Energy, Elsevier, vol. 334(C).
    19. Gonzalo Nahuel Bidart & David Teze & Charlotte Uldahl Jansen & Eleonora Pasutto & Natalia Putkaradze & Anna-Mamusu Sesay & Folmer Fredslund & Leila Lo Leggio & Olafur Ögmundarson & Sumesh Sukumara & K, 2024. "Chemoenzymatic indican for light-driven denim dyeing," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    20. Qing Ma & Yongjun Gao & Bo Sun & Jianlong Du & Hong Zhang & Ding Ma, 2024. "Grave-to-cradle dry reforming of plastics via Joule heating," Nature Communications, Nature, vol. 15(1), pages 1-10, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26508-0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.