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Structural insight into catalytic mechanism of PET hydrolase

Author

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  • Xu Han

    (Chinese Academy of Sciences)

  • Weidong Liu

    (Chinese Academy of Sciences)

  • Jian-Wen Huang

    (AsiaPac Biotechnology Co., Ltd)

  • Jiantao Ma

    (Chinese Academy of Sciences
    Jiangnan University)

  • Yingying Zheng

    (Chinese Academy of Sciences)

  • Tzu-Ping Ko

    (Academia Sinica)

  • Limin Xu

    (AsiaPac Biotechnology Co., Ltd)

  • Ya-Shan Cheng

    (AsiaPac Biotechnology Co., Ltd)

  • Chun-Chi Chen

    (Chinese Academy of Sciences)

  • Rey-Ting Guo

    (Chinese Academy of Sciences)

Abstract

PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and product analogs. Through structural analyses, mutagenesis, and activity measurements, a substrate-binding mode is proposed, and several features critical for catalysis are elucidated.

Suggested Citation

  • Xu Han & Weidong Liu & Jian-Wen Huang & Jiantao Ma & Yingying Zheng & Tzu-Ping Ko & Limin Xu & Ya-Shan Cheng & Chun-Chi Chen & Rey-Ting Guo, 2017. "Structural insight into catalytic mechanism of PET hydrolase," Nature Communications, Nature, vol. 8(1), pages 1-6, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-02255-z
    DOI: 10.1038/s41467-017-02255-z
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    Cited by:

    1. Hwaseok Hong & Dongwoo Ki & Hogyun Seo & Jiyoung Park & Jaewon Jang & Kyung-Jin Kim, 2023. "Discovery and rational engineering of PET hydrolase with both mesophilic and thermophilic PET hydrolase properties," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Yu Yang & Jian Min & Ting Xue & Pengcheng Jiang & Xin Liu & Rouming Peng & Jian-Wen Huang & Yingying Qu & Xian Li & Ning Ma & Fang-Chang Tsai & Longhai Dai & Qi Zhang & Yingle Liu & Chun-Chi Chen & Re, 2023. "Complete bio-degradation of poly(butylene adipate-co-terephthalate) via engineered cutinases," Nature Communications, Nature, vol. 14(1), pages 1-8, December.
    3. Trishnamoni Gautom & Dharmendra Dheeman & Colin Levy & Thomas Butterfield & Guadalupe Alvarez Gonzalez & Philip Roy & Lewis Caiger & Karl Fisher & Linus Johannissen & Neil Dixon, 2021. "Structural basis of terephthalate recognition by solute binding protein TphC," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    4. Zhuozhi Chen & Rongdi Duan & Yunjie Xiao & Yi Wei & Hanxiao Zhang & Xinzhao Sun & Shen Wang & Yingying Cheng & Xue Wang & Shanwei Tong & Yunxiao Yao & Cheng Zhu & Haitao Yang & Yanyan Wang & Zefang Wa, 2022. "Biodegradation of highly crystallized poly(ethylene terephthalate) through cell surface codisplay of bacterial PETase and hydrophobin," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    5. Anni Li & Yijie Sheng & Haiyang Cui & Minghui Wang & Luxuan Wu & Yibo Song & Rongrong Yang & Xiujuan Li & He Huang, 2023. "Discovery and mechanism-guided engineering of BHET hydrolases for improved PET recycling and upcycling," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    6. P. Konstantin Richter & Paula Blázquez-Sánchez & Ziyue Zhao & Felipe Engelberger & Christian Wiebeler & Georg Künze & Ronny Frank & Dana Krinke & Emanuele Frezzotti & Yuliia Lihanova & Patricia Falken, 2023. "Structure and function of the metagenomic plastic-degrading polyester hydrolase PHL7 bound to its product," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    7. Erika Erickson & Japheth E. Gado & Luisana Avilán & Felicia Bratti & Richard K. Brizendine & Paul A. Cox & Raj Gill & Rosie Graham & Dong-Jin Kim & Gerhard König & William E. Michener & Saroj Poudel &, 2022. "Sourcing thermotolerant poly(ethylene terephthalate) hydrolase scaffolds from natural diversity," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

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