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Light-triggered and phosphorylation-dependent 14-3-3 association with NON-PHOTOTROPIC HYPOCOTYL 3 is required for hypocotyl phototropism

Author

Listed:
  • Lea Reuter

    (University of Tübingen)

  • Tanja Schmidt

    (University of Tübingen)

  • Prabha Manishankar

    (University of Tübingen)

  • Christian Throm

    (University of Tübingen)

  • Jutta Keicher

    (University of Tübingen)

  • Andrea Bock

    (University of Tübingen)

  • Irina Droste-Borel

    (University of Tübingen)

  • Claudia Oecking

    (University of Tübingen)

Abstract

NON-PHOTOTROPIC HYPOCOTYL 3 (NPH3) is a key component of the auxin-dependent plant phototropic growth response. We report that NPH3 directly binds polyacidic phospholipids, required for plasma membrane association in darkness. We further demonstrate that blue light induces an immediate phosphorylation of a C-terminal 14-3-3 binding motif in NPH3. Subsequent association of 14-3-3 proteins is causal for the light-induced release of NPH3 from the membrane and accompanied by NPH3 dephosphorylation. In the cytosol, NPH3 dynamically transitions into membraneless condensate-like structures. The dephosphorylated state of the 14-3-3 binding site and NPH3 membrane recruitment are recoverable in darkness. NPH3 variants that constitutively localize either to the membrane or to condensates are non-functional, revealing a fundamental role of the 14-3-3 mediated dynamic change in NPH3 localization for auxin-dependent phototropism. This regulatory mechanism might be of general nature, given that several members of the NPH3-like family interact with 14-3-3 via a C-terminal motif.

Suggested Citation

  • Lea Reuter & Tanja Schmidt & Prabha Manishankar & Christian Throm & Jutta Keicher & Andrea Bock & Irina Droste-Borel & Claudia Oecking, 2021. "Light-triggered and phosphorylation-dependent 14-3-3 association with NON-PHOTOTROPIC HYPOCOTYL 3 is required for hypocotyl phototropism," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26332-6
    DOI: 10.1038/s41467-021-26332-6
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    References listed on IDEAS

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    1. Paolo Schumacher & Emilie Demarsy & Patrice Waridel & Laure Allenbach Petrolati & Martine Trevisan & Christian Fankhauser, 2018. "A phosphorylation switch turns a positive regulator of phototropism into an inhibitor of the process," Nature Communications, Nature, vol. 9(1), pages 1-9, December.
    2. Julia Mergner & Martin Frejno & Markus List & Michael Papacek & Xia Chen & Ajeet Chaudhary & Patroklos Samaras & Sandra Richter & Hiromasa Shikata & Maxim Messerer & Daniel Lang & Stefan Altmann & Phi, 2020. "Mass-spectrometry-based draft of the Arabidopsis proteome," Nature, Nature, vol. 579(7799), pages 409-414, March.
    3. Asami Hiyama & Atsushi Takemiya & Shintaro Munemasa & Eiji Okuma & Naoyuki Sugiyama & Yasuomi Tada & Yoshiyuki Murata & Ken-ichiro Shimazaki, 2017. "Blue light and CO2 signals converge to regulate light-induced stomatal opening," Nature Communications, Nature, vol. 8(1), pages 1-13, December.
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