IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-26207-w.html
   My bibliography  Save this article

Association of snR190 snoRNA chaperone with early pre-60S particles is regulated by the RNA helicase Dbp7 in yeast

Author

Listed:
  • Mariam Jaafar

    (Université de Toulouse, CNRS, UPS
    Lebanese University
    Cancer Research Center of Lyon (CRCL))

  • Julia Contreras

    (Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla
    Universidad de Sevilla)

  • Carine Dominique

    (Université de Toulouse, CNRS, UPS)

  • Sara Martín-Villanueva

    (Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla)

  • Régine Capeyrou

    (Université de Toulouse, CNRS, UPS)

  • Patrice Vitali

    (Université de Toulouse, CNRS, UPS)

  • Olga Rodríguez-Galán

    (Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla
    Universidad de Sevilla)

  • Carmen Velasco

    (Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla
    Universidad de Sevilla)

  • Odile Humbert

    (Université de Toulouse, CNRS, UPS)

  • Nicholas J. Watkins

    (Newcastle University)

  • Eduardo Villalobo

    (Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla
    Universidad de Sevilla)

  • Katherine E. Bohnsack

    (University Medical Centre Göttingen)

  • Markus T. Bohnsack

    (University Medical Centre Göttingen
    Georg-August University Göttingen)

  • Yves Henry

    (Université de Toulouse, CNRS, UPS)

  • Raghida Abou Merhi

    (Lebanese University)

  • Jesús de la Cruz

    (Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla
    Universidad de Sevilla)

  • Anthony K. Henras

    (Université de Toulouse, CNRS, UPS)

Abstract

Synthesis of eukaryotic ribosomes involves the assembly and maturation of precursor particles (pre-ribosomal particles) containing ribosomal RNA (rRNA) precursors, ribosomal proteins (RPs) and a plethora of assembly factors (AFs). Formation of the earliest precursors of the 60S ribosomal subunit (pre-60S r-particle) is among the least understood stages of ribosome biogenesis. It involves the Npa1 complex, a protein module suggested to play a key role in the early structuring of the pre-rRNA. Npa1 displays genetic interactions with the DExD-box protein Dbp7 and interacts physically with the snR190 box C/D snoRNA. We show here that snR190 functions as a snoRNA chaperone, which likely cooperates with the Npa1 complex to initiate compaction of the pre-rRNA in early pre-60S r-particles. We further show that Dbp7 regulates the dynamic base-pairing between snR190 and the pre-rRNA within the earliest pre-60S r-particles, thereby participating in structuring the peptidyl transferase center (PTC) of the large ribosomal subunit.

Suggested Citation

  • Mariam Jaafar & Julia Contreras & Carine Dominique & Sara Martín-Villanueva & Régine Capeyrou & Patrice Vitali & Olga Rodríguez-Galán & Carmen Velasco & Odile Humbert & Nicholas J. Watkins & Eduardo V, 2021. "Association of snR190 snoRNA chaperone with early pre-60S particles is regulated by the RNA helicase Dbp7 in yeast," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26207-w
    DOI: 10.1038/s41467-021-26207-w
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-26207-w
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-26207-w?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Brian T. Wimberly & Ditlev E. Brodersen & William M. Clemons & Robert J. Morgan-Warren & Andrew P. Carter & Clemens Vonrhein & Thomas Hartsch & V. Ramakrishnan, 2000. "Structure of the 30S ribosomal subunit," Nature, Nature, vol. 407(6802), pages 327-339, September.
    2. Elena Burlacu & Fredrik Lackmann & Lisbeth-Carolina Aguilar & Sergey Belikov & Rob van Nues & Christian Trahan & Ralph D. Hector & Nicholas Dominelli-Whiteley & Scott L. Cockroft & Lars Wieslander & M, 2017. "High-throughput RNA structure probing reveals critical folding events during early 60S ribosome assembly in yeast," Nature Communications, Nature, vol. 8(1), pages 1-14, December.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Gerald Ryan R. Aquino & Philipp Hackert & Nicolai Krogh & Kuan-Ting Pan & Mariam Jaafar & Anthony K. Henras & Henrik Nielsen & Henning Urlaub & Katherine E. Bohnsack & Markus T. Bohnsack, 2021. "The RNA helicase Dbp7 promotes domain V/VI compaction and stabilization of inter-domain interactions during early 60S assembly," Nature Communications, Nature, vol. 12(1), pages 1-16, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Simon A. Fromm & Kate M. O’Connor & Michael Purdy & Pramod R. Bhatt & Gary Loughran & John F. Atkins & Ahmad Jomaa & Simone Mattei, 2023. "The translating bacterial ribosome at 1.55 Å resolution generated by cryo-EM imaging services," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    2. Rebecca S Mathew-Fenn & Rhiju Das & Joshua A Silverman & Peter A Walker & Pehr A B Harbury, 2008. "A Molecular Ruler for Measuring Quantitative Distance Distributions," PLOS ONE, Public Library of Science, vol. 3(10), pages 1-9, October.
    3. Gerald Ryan R. Aquino & Philipp Hackert & Nicolai Krogh & Kuan-Ting Pan & Mariam Jaafar & Anthony K. Henras & Henrik Nielsen & Henning Urlaub & Katherine E. Bohnsack & Markus T. Bohnsack, 2021. "The RNA helicase Dbp7 promotes domain V/VI compaction and stabilization of inter-domain interactions during early 60S assembly," Nature Communications, Nature, vol. 12(1), pages 1-16, December.
    4. Meagan C Small & Pedro Lopes & Rodrigo B Andrade & Alexander D MacKerell Jr, 2013. "Impact of Ribosomal Modification on the Binding of the Antibiotic Telithromycin Using a Combined Grand Canonical Monte Carlo/Molecular Dynamics Simulation Approach," PLOS Computational Biology, Public Library of Science, vol. 9(6), pages 1-14, June.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26207-w. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.