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Association of snR190 snoRNA chaperone with early pre-60S particles is regulated by the RNA helicase Dbp7 in yeast

Author

Listed:
  • Mariam Jaafar

    (Université de Toulouse, CNRS, UPS
    Lebanese University
    Cancer Research Center of Lyon (CRCL))

  • Julia Contreras

    (Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla
    Universidad de Sevilla)

  • Carine Dominique

    (Université de Toulouse, CNRS, UPS)

  • Sara Martín-Villanueva

    (Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla)

  • Régine Capeyrou

    (Université de Toulouse, CNRS, UPS)

  • Patrice Vitali

    (Université de Toulouse, CNRS, UPS)

  • Olga Rodríguez-Galán

    (Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla
    Universidad de Sevilla)

  • Carmen Velasco

    (Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla
    Universidad de Sevilla)

  • Odile Humbert

    (Université de Toulouse, CNRS, UPS)

  • Nicholas J. Watkins

    (Newcastle University)

  • Eduardo Villalobo

    (Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla
    Universidad de Sevilla)

  • Katherine E. Bohnsack

    (University Medical Centre Göttingen)

  • Markus T. Bohnsack

    (University Medical Centre Göttingen
    Georg-August University Göttingen)

  • Yves Henry

    (Université de Toulouse, CNRS, UPS)

  • Raghida Abou Merhi

    (Lebanese University)

  • Jesús de la Cruz

    (Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla
    Universidad de Sevilla)

  • Anthony K. Henras

    (Université de Toulouse, CNRS, UPS)

Abstract

Synthesis of eukaryotic ribosomes involves the assembly and maturation of precursor particles (pre-ribosomal particles) containing ribosomal RNA (rRNA) precursors, ribosomal proteins (RPs) and a plethora of assembly factors (AFs). Formation of the earliest precursors of the 60S ribosomal subunit (pre-60S r-particle) is among the least understood stages of ribosome biogenesis. It involves the Npa1 complex, a protein module suggested to play a key role in the early structuring of the pre-rRNA. Npa1 displays genetic interactions with the DExD-box protein Dbp7 and interacts physically with the snR190 box C/D snoRNA. We show here that snR190 functions as a snoRNA chaperone, which likely cooperates with the Npa1 complex to initiate compaction of the pre-rRNA in early pre-60S r-particles. We further show that Dbp7 regulates the dynamic base-pairing between snR190 and the pre-rRNA within the earliest pre-60S r-particles, thereby participating in structuring the peptidyl transferase center (PTC) of the large ribosomal subunit.

Suggested Citation

  • Mariam Jaafar & Julia Contreras & Carine Dominique & Sara Martín-Villanueva & Régine Capeyrou & Patrice Vitali & Olga Rodríguez-Galán & Carmen Velasco & Odile Humbert & Nicholas J. Watkins & Eduardo V, 2021. "Association of snR190 snoRNA chaperone with early pre-60S particles is regulated by the RNA helicase Dbp7 in yeast," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26207-w
    DOI: 10.1038/s41467-021-26207-w
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    References listed on IDEAS

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    1. Brian T. Wimberly & Ditlev E. Brodersen & William M. Clemons & Robert J. Morgan-Warren & Andrew P. Carter & Clemens Vonrhein & Thomas Hartsch & V. Ramakrishnan, 2000. "Structure of the 30S ribosomal subunit," Nature, Nature, vol. 407(6802), pages 327-339, September.
    2. Elena Burlacu & Fredrik Lackmann & Lisbeth-Carolina Aguilar & Sergey Belikov & Rob van Nues & Christian Trahan & Ralph D. Hector & Nicholas Dominelli-Whiteley & Scott L. Cockroft & Lars Wieslander & M, 2017. "High-throughput RNA structure probing reveals critical folding events during early 60S ribosome assembly in yeast," Nature Communications, Nature, vol. 8(1), pages 1-14, December.
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    Cited by:

    1. Gerald Ryan R. Aquino & Philipp Hackert & Nicolai Krogh & Kuan-Ting Pan & Mariam Jaafar & Anthony K. Henras & Henrik Nielsen & Henning Urlaub & Katherine E. Bohnsack & Markus T. Bohnsack, 2021. "The RNA helicase Dbp7 promotes domain V/VI compaction and stabilization of inter-domain interactions during early 60S assembly," Nature Communications, Nature, vol. 12(1), pages 1-16, December.

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