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NIR-II bioluminescence for in vivo high contrast imaging and in situ ATP-mediated metastases tracing

Author

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  • Lingfei Lu

    (Fudan University)

  • Benhao Li

    (Fudan University)

  • Suwan Ding

    (Fudan University)

  • Yong Fan

    (Fudan University)

  • Shangfeng Wang

    (Fudan University)

  • Caixia Sun

    (Fudan University)

  • Mengyao Zhao

    (Fudan University)

  • Chun-Xia Zhao

    (The University of Queensland)

  • Fan Zhang

    (Fudan University)

Abstract

Bioluminescence imaging has been widely used in life sciences and biomedical applications. However, conventional bioluminescence imaging usually operates in the visible region, which hampers the high-performance in vivo optical imaging due to the strong tissue absorption and scattering. To address this challenge, here we present bioluminescence probes (BPs) with emission in the second near infrared (NIR-II) region at 1029 nm by employing bioluminescence resonance energy transfer (BRET) and two-step fluorescence resonance energy transfer (FRET) with a specially designed cyanine dye FD-1029. The biocompatible NIR-II-BPs are successfully applied to vessels and lymphatics imaging in mice, which gives ~5 times higher signal-to-noise ratios and ~1.5 times higher spatial resolution than those obtained by NIR-II fluorescence imaging and conventional bioluminescence imaging. Their capability of multiplexed imaging is also well displayed. Taking advantage of the ATP-responding character, the NIR-II-BPs are able to recognize tumor metastasis with a high tumor-to-normal tissue ratio at 83.4.

Suggested Citation

  • Lingfei Lu & Benhao Li & Suwan Ding & Yong Fan & Shangfeng Wang & Caixia Sun & Mengyao Zhao & Chun-Xia Zhao & Fan Zhang, 2020. "NIR-II bioluminescence for in vivo high contrast imaging and in situ ATP-mediated metastases tracing," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18051-1
    DOI: 10.1038/s41467-020-18051-1
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    Cited by:

    1. Yuling Xu & Chonglu Li & Shuai Lu & Zhizheng Wang & Shuang Liu & Xiujun Yu & Xiaopeng Li & Yao Sun, 2022. "Construction of emissive ruthenium(II) metallacycle over 1000 nm wavelength for in vivo biomedical applications," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    2. Qian Zhang & Bin Song & Yanan Xu & Yunmin Yang & Jian Ji & Wenjun Cao & Jianping Lu & Jiali Ding & Haiting Cao & Binbin Chu & Jiaxu Hong & Houyu Wang & Yao He, 2023. "In vivo bioluminescence imaging of natural bacteria within deep tissues via ATP-binding cassette sugar transporter," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    3. Yongchao Liu & Lili Teng & Yifan Lyu & Guosheng Song & Xiao-Bing Zhang & Weihong Tan, 2022. "Ratiometric afterglow luminescent nanoplatform enables reliable quantification and molecular imaging," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    4. Yue Jiang & Min Zhao & Jia Miao & Wan Chen & Yuan Zhang & Minqian Miao & Li Yang & Qing Li & Qingqing Miao, 2024. "Acidity-activatable upconversion afterglow luminescence cocktail nanoparticles for ultrasensitive in vivo imaging," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    5. Dongsheng Tang & Minhui Cui & Bin Wang & Ganghao Liang & Hanchen Zhang & Haihua Xiao, 2024. "Nanoparticles destabilizing the cell membranes triggered by NIR light for cancer imaging and photo-immunotherapy," Nature Communications, Nature, vol. 15(1), pages 1-21, December.

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