IDEAS home Printed from https://ideas.repec.org/a/gam/jsusta/v16y2024i13p5284-d1419484.html
   My bibliography  Save this article

Effect of Extending High-Temperature Duration on ARG Rebound in a Co-Composting Process for Organic Wastes

Author

Listed:
  • Xi Yang

    (College of Life Sciences, Wuhan University, 299 Bayi Road, Wuhan 430072, China)

  • Pengyu Sun

    (School of Environmental Science and Engineering, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, China)

  • Botao Liu

    (School of Environmental Science and Engineering, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, China)

  • Imtiaz Ahmed

    (School of Environmental Science and Engineering, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, China)

  • Zhixiong Xie

    (College of Life Sciences, Wuhan University, 299 Bayi Road, Wuhan 430072, China)

  • Bo Zhang

    (School of Environmental Science and Engineering, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, China)

Abstract

High levels of antibiotic resistance genes (ARGs) in compost materials pose a significant threat to the environment and human health. Conventional composting (CC) is widely adopted for waste management. However, mitigating ARG rebound in the late phase remains challenging. This work presents a strategy to extend the high-temperature duration by external heating to achieve rapid composting (RC). An innovative two-stage heating mode (first stage: day 3–6, 55 °C; second stage: day 7–10, 70 °C) was utilized in this study. We aimed to compare the removal and the rebound of ARGs and mobile genetic elements (MGEs) between RC and CC treatments and to identify the key factors driving the fate of ARGs throughout the composting process by integrating with environmental factors, external stress, MGEs, and microbial communities. The results show that on day 40, ARGs increased by 8.2 times in conventional composting. After the high-temperature duration was prolonged from 5 days to 9 days, the highest elimination rates achieved for ARGs and MGEs were 85% and 97%, respectively; concurrently, ARG rebound was suppressed compared to conventional composting. Genes resisting β-lactamase, chloramphenicol, and quinolone showed maximal removal in both treatments. The antibiotics showed a significant reduction in both treatments, with 79.3% in extended high-temperature duration composting and 75.26% in conventional composting. Network analysis revealed that Gammaproteobacteria , Clostridia , Saccharimonadia , Cyanobacteriia , and Campylobacteria were the potential hosts of various ARG subtypes, and their abundance was reduced in extended high-temperature duration composting. Redundancy analysis (RDA) and structural equation model (SEM) confirmed that temperature was the key factor in composting, while the potential hosts of MGEs and ARGs were responsible for the rebounding of ARGs in conventional composting. Prolonging composting temperature is a key strategy for the removal of contaminants from aerobic composting to achieve a safe end-product.

Suggested Citation

  • Xi Yang & Pengyu Sun & Botao Liu & Imtiaz Ahmed & Zhixiong Xie & Bo Zhang, 2024. "Effect of Extending High-Temperature Duration on ARG Rebound in a Co-Composting Process for Organic Wastes," Sustainability, MDPI, vol. 16(13), pages 1-15, June.
  • Handle: RePEc:gam:jsusta:v:16:y:2024:i:13:p:5284-:d:1419484
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2071-1050/16/13/5284/pdf
    Download Restriction: no

    File URL: https://www.mdpi.com/2071-1050/16/13/5284/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Qingxiang Yang & Hao Zhang & Yuhui Guo & Tiantian Tian, 2016. "Influence of Chicken Manure Fertilization on Antibiotic-Resistant Bacteria in Soil and the Endophytic Bacteria of Pakchoi," IJERPH, MDPI, vol. 13(7), pages 1-12, June.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Zhikang Wang & Ziyun Chen & Xiangxiang Fu, 2019. "Integrated Effects of Co-Inoculation with Phosphate-Solubilizing Bacteria and N 2 -Fixing Bacteria on Microbial Population and Soil Amendment Under C Deficiency," IJERPH, MDPI, vol. 16(13), pages 1-15, July.
    2. Hao Zhang & Xunan Li & Qingxiang Yang & Linlin Sun & Xinxin Yang & Mingming Zhou & Rongzhen Deng & Linqian Bi, 2017. "Plant Growth, Antibiotic Uptake, and Prevalence of Antibiotic Resistance in an Endophytic System of Pakchoi under Antibiotic Exposure," IJERPH, MDPI, vol. 14(11), pages 1-12, November.
    3. Xiujuan Chen & Linhai Wu & Xuyan Xie, 2018. "Assessing the Linkages between Knowledge and Use of Veterinary Antibiotics by Pig Farmers in Rural China," IJERPH, MDPI, vol. 15(6), pages 1-13, May.
    4. Karina Yévenes & Ekaterina Pokrant & Fernando Pérez & Ricardo Riquelme & Constanza Avello & Aldo Maddaleno & Betty San Martín & Javiera Cornejo, 2018. "Assessment of Three Antimicrobial Residue Concentrations in Broiler Chicken Droppings as a Potential Risk Factor for Public Health and Environment," IJERPH, MDPI, vol. 16(1), pages 1-13, December.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jsusta:v:16:y:2024:i:13:p:5284-:d:1419484. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.