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Influence of Chicken Manure Fertilization on Antibiotic-Resistant Bacteria in Soil and the Endophytic Bacteria of Pakchoi

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  • Qingxiang Yang

    (School of Environment, Henan Normal University, Xinxiang 453007, China
    College of Life Sciences, Henan Normal University, Xinxiang 453007, China
    Key Laboratory for Microorganisms and Functional Molecules, University of Henan Province, Xinxiang 453007, China)

  • Hao Zhang

    (School of Environment, Henan Normal University, Xinxiang 453007, China
    College of Life Sciences, Henan Normal University, Xinxiang 453007, China
    Key Laboratory for Microorganisms and Functional Molecules, University of Henan Province, Xinxiang 453007, China)

  • Yuhui Guo

    (College of Life Sciences, Henan Normal University, Xinxiang 453007, China)

  • Tiantian Tian

    (College of Life Sciences, Henan Normal University, Xinxiang 453007, China)

Abstract

Animal manure is commonly used as fertilizer for agricultural crops worldwide, even though it is believed to contribute to the spread of antibiotic resistance from animal intestines to the soil environment. However, it is unclear whether and how there is any impact of manure fertilization on populations and community structure of antibiotic-resistant endophytic bacteria (AREB) in plant tissues. To investigate the effect of manure and organic fertilizer on endophytic bacterial communities, pot experiments were performed with pakchoi grown with the following treatments: (1) non-treated; (2) chicken manure-treated and (3) organic fertilizer-treated. Manure or organic fertilizer significantly increased the abundances of total cultivable endophytic bacteria (TCEB) and AREB in pakchoi, and the effect of chicken manure was greater than that of organic fertilizer. Further, 16S rDNA sequencing and the phylogenetic analysis indicated that chicken manure or organic fertilizer application increased the populations of multiple antibiotic-resistant bacteria (MARB) in soil and multiple antibiotic-resistant endophytic bacteria (MAREB) in pakchoi. The identical multiple antibiotic-resistant bacterial populations detected in chicken manure, manure- or organic fertilizer-amended soil and the vegetable endophytic system were Brevundimonas diminuta , Brachybacterium sp. and Bordetella sp., suggesting that MARB from manure could enter and colonize the vegetable tissues through manure fertilization. The fact that some human pathogens with multiple antibiotic resistance were detected in harvested vegetables after growing in manure-amended soil demonstrated a potential threat to human health.

Suggested Citation

  • Qingxiang Yang & Hao Zhang & Yuhui Guo & Tiantian Tian, 2016. "Influence of Chicken Manure Fertilization on Antibiotic-Resistant Bacteria in Soil and the Endophytic Bacteria of Pakchoi," IJERPH, MDPI, vol. 13(7), pages 1-12, June.
    • Handle: RePEc:gam:jijerp:v:13:y:2016:i:7:p:662-:d:73120
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    Citations

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    Cited by:

    1. Karina Yévenes & Ekaterina Pokrant & Fernando Pérez & Ricardo Riquelme & Constanza Avello & Aldo Maddaleno & Betty San Martín & Javiera Cornejo, 2018. "Assessment of Three Antimicrobial Residue Concentrations in Broiler Chicken Droppings as a Potential Risk Factor for Public Health and Environment," IJERPH, MDPI, vol. 16(1), pages 1-13, December.
    2. Zhikang Wang & Ziyun Chen & Xiangxiang Fu, 2019. "Integrated Effects of Co-Inoculation with Phosphate-Solubilizing Bacteria and N 2 -Fixing Bacteria on Microbial Population and Soil Amendment Under C Deficiency," IJERPH, MDPI, vol. 16(13), pages 1-15, July.
    3. Hao Zhang & Xunan Li & Qingxiang Yang & Linlin Sun & Xinxin Yang & Mingming Zhou & Rongzhen Deng & Linqian Bi, 2017. "Plant Growth, Antibiotic Uptake, and Prevalence of Antibiotic Resistance in an Endophytic System of Pakchoi under Antibiotic Exposure," IJERPH, MDPI, vol. 14(11), pages 1-12, November.
    4. Xiujuan Chen & Linhai Wu & Xuyan Xie, 2018. "Assessing the Linkages between Knowledge and Use of Veterinary Antibiotics by Pig Farmers in Rural China," IJERPH, MDPI, vol. 15(6), pages 1-13, May.
    5. Xi Yang & Pengyu Sun & Botao Liu & Imtiaz Ahmed & Zhixiong Xie & Bo Zhang, 2024. "Effect of Extending High-Temperature Duration on ARG Rebound in a Co-Composting Process for Organic Wastes," Sustainability, MDPI, vol. 16(13), pages 1-15, June.

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