IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v14y2017i11p1336-d117463.html
   My bibliography  Save this article

Plant Growth, Antibiotic Uptake, and Prevalence of Antibiotic Resistance in an Endophytic System of Pakchoi under Antibiotic Exposure

Author

Listed:
  • Hao Zhang

    (School of Environment, Henan Normal University, Xinxiang 453007, China
    College of Life Sciences, Henan Normal University, Xinxiang 453007, China
    Key Laboratory for Microorganisms and Functional Molecules, University of Henan Province, Xinxiang 453007, China)

  • Xunan Li

    (College of Life Sciences, Henan Normal University, Xinxiang 453007, China)

  • Qingxiang Yang

    (School of Environment, Henan Normal University, Xinxiang 453007, China
    College of Life Sciences, Henan Normal University, Xinxiang 453007, China
    Key Laboratory for Microorganisms and Functional Molecules, University of Henan Province, Xinxiang 453007, China)

  • Linlin Sun

    (College of Life Sciences, Henan Normal University, Xinxiang 453007, China)

  • Xinxin Yang

    (College of Life Sciences, Henan Normal University, Xinxiang 453007, China)

  • Mingming Zhou

    (College of Life Sciences, Henan Normal University, Xinxiang 453007, China)

  • Rongzhen Deng

    (College of Life Sciences, Henan Normal University, Xinxiang 453007, China)

  • Linqian Bi

    (College of Life Sciences, Henan Normal University, Xinxiang 453007, China)

Abstract

Antibiotic contamination in agroecosystems may cause serious problems, such as the proliferation of various antibiotic resistant bacteria and the spreading of antibiotic resistance genes (ARGs) in the environment or even to human beings. However, it is unclear whether environmental antibiotics, antibiotic resistant bacteria, and ARGs can directly enter into, or occur in, the endophytic systems of plants exposed to pollutants. In this study, a hydroponic experiment exposing pakchoi ( Brassica chinensis L.) to tetracycline, cephalexin, and sulfamethoxazole at 50% minimum inhibitory concentration (MIC) levels and MIC levels, respectively, was conducted to explore plant growth, antibiotic uptake, and the development of antibiotic resistance in endophytic systems. The three antibiotics promoted pakchoi growth at 50% MIC values. Target antibiotics at concentrations ranging from 6.9 to 48.1 µg·kg −1 were detected in the treated vegetables. Additionally, the rates of antibiotic-resistant endophytic bacteria to total cultivable endophytic bacteria significantly increased as the antibiotics accumulated in the plants. The detection and quantification of ARGs indicated that four types, tet X, bla CTX-M , and sul 1 and sul 2, which correspond to tetracycline, cephalexin, and sulfamethoxazole resistance, respectively, were present in the pakchoi endophytic system and increased with the antibiotic concentrations. The results highlight a potential risk of the development and spread of antibiotic resistance in vegetable endophytic systems.

Suggested Citation

  • Hao Zhang & Xunan Li & Qingxiang Yang & Linlin Sun & Xinxin Yang & Mingming Zhou & Rongzhen Deng & Linqian Bi, 2017. "Plant Growth, Antibiotic Uptake, and Prevalence of Antibiotic Resistance in an Endophytic System of Pakchoi under Antibiotic Exposure," IJERPH, MDPI, vol. 14(11), pages 1-12, November.
  • Handle: RePEc:gam:jijerp:v:14:y:2017:i:11:p:1336-:d:117463
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/14/11/1336/pdf
    Download Restriction: no

    File URL: https://www.mdpi.com/1660-4601/14/11/1336/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Qingxiang Yang & Hao Zhang & Yuhui Guo & Tiantian Tian, 2016. "Influence of Chicken Manure Fertilization on Antibiotic-Resistant Bacteria in Soil and the Endophytic Bacteria of Pakchoi," IJERPH, MDPI, vol. 13(7), pages 1-12, June.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Małgorzata Czatzkowska & Izabela Wolak & Monika Harnisz & Ewa Korzeniewska, 2022. "Impact of Anthropogenic Activities on the Dissemination of ARGs in the Environment—A Review," IJERPH, MDPI, vol. 19(19), pages 1-29, October.
    2. Bingbing Du & Qingxiang Yang & Ruifei Wang & Ruimin Wang & Qiang Wang & Yuan Xin, 2019. "Evolution of Antibiotic Resistance and the Relationship between the Antibiotic Resistance Genes and Microbial Compositions under Long-Term Exposure to Tetracycline and Sulfamethoxazole," IJERPH, MDPI, vol. 16(23), pages 1-15, November.
    3. Magdalena Krupka & Ewa Olkowska & Agnieszka Klimkowicz-Pawlas & Leszek Łęczyński & Maciej Tankiewicz & Dariusz J. Michalczyk & Lidia Wolska & Agnieszka I. Piotrowicz-Cieślak, 2023. "The Impact of Soil and Water Pollutants Released from Poultry Farming on the Growth and Development of Two Plant Species," Agriculture, MDPI, vol. 14(1), pages 1-22, December.
    4. Ashok J. Tamhankar & Cecilia Stålsby Lundborg, 2019. "Antimicrobials and Antimicrobial Resistance in the Environment and Its Remediation: A Global One Health Perspective," IJERPH, MDPI, vol. 16(23), pages 1-7, November.
    5. Junjun Ma & Bing Li & Lincheng Zhou & Yin Zhu & Ji Li & Yong Qiu, 2018. "Simple Urea Immersion Enhanced Removal of Tetracycline from Water by Polystyrene Microspheres," IJERPH, MDPI, vol. 15(7), pages 1-15, July.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Zhikang Wang & Ziyun Chen & Xiangxiang Fu, 2019. "Integrated Effects of Co-Inoculation with Phosphate-Solubilizing Bacteria and N 2 -Fixing Bacteria on Microbial Population and Soil Amendment Under C Deficiency," IJERPH, MDPI, vol. 16(13), pages 1-15, July.
    2. Xiujuan Chen & Linhai Wu & Xuyan Xie, 2018. "Assessing the Linkages between Knowledge and Use of Veterinary Antibiotics by Pig Farmers in Rural China," IJERPH, MDPI, vol. 15(6), pages 1-13, May.
    3. Xi Yang & Pengyu Sun & Botao Liu & Imtiaz Ahmed & Zhixiong Xie & Bo Zhang, 2024. "Effect of Extending High-Temperature Duration on ARG Rebound in a Co-Composting Process for Organic Wastes," Sustainability, MDPI, vol. 16(13), pages 1-15, June.
    4. Karina Yévenes & Ekaterina Pokrant & Fernando Pérez & Ricardo Riquelme & Constanza Avello & Aldo Maddaleno & Betty San Martín & Javiera Cornejo, 2018. "Assessment of Three Antimicrobial Residue Concentrations in Broiler Chicken Droppings as a Potential Risk Factor for Public Health and Environment," IJERPH, MDPI, vol. 16(1), pages 1-13, December.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:14:y:2017:i:11:p:1336-:d:117463. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.