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Pollutant-Removing Biofilter Strains Associated with High Ammonia and Hydrogen Sulfide Removal Rate in a Livestock Wastewater Treatment Facility

Author

Listed:
  • Dong-Hyun Kim

    (School of Applied Biosciences, Kyungpook National University, Daegu, 37224, Korea
    These authors contributed equally to this work.)

  • Hyun-Sik Yun

    (Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu 41566, Korea
    These authors contributed equally to this work.)

  • Young-Saeng Kim

    (Research Institute of Ulleung-do & Dok-do, Kyungpook National University, Daegu 41566, Korea)

  • Jong-Guk Kim

    (School of Applied Biosciences, Kyungpook National University, Daegu, 37224, Korea
    School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, Korea)

Abstract

This study analyzed the microbial community metagenomically to determine the cause of the functionality of a livestock wastewater treatment facility that can effectively remove pollutants, such as ammonia and hydrogen sulfide. Illumina MiSeq sequencing was used in analyzing the composition and structure of the microbial community, and the 16S rRNA gene was used. Through Illumina MiSeq sequencing, information such as diversity indicators as well as the composition and structure of microbial communities present in the livestock wastewater treatment facility were obtained, and differences between microbial communities present in the investigated samples were compared. The number of reads, operational taxonomic units, and species richness were lower in influent sample (NLF), where the wastewater enters, than in effluent sample (NL), in which treated wastewater is found. This difference was greater in June 2019 than in January 2020, and the removal rates of ammonia (86.93%) and hydrogen sulfide (99.72%) were also higher in June 2019. In both areas, the community composition was similar in January 2020, whereas the influent sample (NLF) and effluent sample (NL) areas in June 2019 were dominated by Proteobacteria (76.23%) and Firmicutes (67.13%), respectively. Oleiphilaceae (40.89%) and Thioalkalibacteraceae (12.91%), which are related to ammonia and hydrogen sulfide removal, respectively, were identified in influent sample (NLF) in June 2019. They were more abundant in June 2019 than in January 2020. Therefore, the functionality of the livestock wastewater treatment facility was affected by characteristics, including the composition of the microbial community. Compared to Illumina MiSeq sequencing, fewer species were isolated and identified in both areas using culture-based methods, suggesting Illumina MiSeq sequencing as a powerful tool to determine the relevance of microbial communities for pollutant removal.

Suggested Citation

  • Dong-Hyun Kim & Hyun-Sik Yun & Young-Saeng Kim & Jong-Guk Kim, 2021. "Pollutant-Removing Biofilter Strains Associated with High Ammonia and Hydrogen Sulfide Removal Rate in a Livestock Wastewater Treatment Facility," Sustainability, MDPI, vol. 13(13), pages 1-16, June.
    • Handle: RePEc:gam:jsusta:v:13:y:2021:i:13:p:7358-:d:586165
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    References listed on IDEAS

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    1. Sean R Eddy, 2011. "Accelerated Profile HMM Searches," PLOS Computational Biology, Public Library of Science, vol. 7(10), pages 1-16, October.
    2. Byunghan Lee & Taesup Moon & Sungroh Yoon & Tsachy Weissman, 2017. "DUDE-Seq: Fast, flexible, and robust denoising for targeted amplicon sequencing," PLOS ONE, Public Library of Science, vol. 12(7), pages 1-25, July.
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    Cited by:

    1. Xintian Li & Wei Zhai & Xinran Duan & Changlong Gou & Min Li & Lixia Wang & Wangdui Basang & Yanbin Zhu & Yunhang Gao, 2022. "Extraction, Purification, Characterization and Application in Livestock Wastewater of S Sulfur Convertase," IJERPH, MDPI, vol. 19(23), pages 1-14, December.

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