IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v18y2021i24p13246-d703586.html
   My bibliography  Save this article

Early Diagnosis of Oral Mucosal Alterations in Smokers and E-Cigarette Users Based on Micronuclei Count: A Cross-Sectional Study among Dental Students

Author

Listed:
  • Anca Maria Pop

    (Faculty of Medicine, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mureș, 540139 Tirgu Mures, Romania)

  • Raluca Coroș

    (Faculty of Dental Medicine, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mureș, 540139 Tirgu Mures, Romania)

  • Alexandra Mihaela Stoica

    (Department of Odontology and Oral Pathology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mureș, 540139 Tirgu Mures, Romania)

  • Monica Monea

    (Department of Odontology and Oral Pathology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mureș, 540139 Tirgu Mures, Romania)

Abstract

The presence of micronuclei in oral epithelial cells is considered a marker of genotoxicity, which can be identified using exfoliative cytology. The aim of this study was to investigate cytotoxic damage through the evaluation of micronuclei in the oral mucosa of smokers and e-cigarette users compared to nonsmokers. We obtained smears from the buccal mucosa of 68 participants divided in 3 groups (smokers, e-cigarette users and nonsmokers), which were further processed with Papanicolaou stain. The frequencies of micronuclei and micronucleated cells were recorded and statistically analyzed at a level of significance of p < 0.05. The mean micronuclei values per 1000 cells were 3.6 ± 1.08 for smokers, 3.21 ± 1.12 for e-cigarette users and 1.95 ± 1.05 for nonsmokers. The mean values of micronucleated cells per 1000 cells were 2.48 ± 0.91 for smokers, 2.39 ± 1.07 for e-cigarette users and 1.4 ± 0.68 for nonsmokers. Smokers and e-cigarette users had significantly higher values of micronuclei and micronucleated cells compared to nonsmokers, but there were no significant differences between smokers and e-cigarette users. We concluded that the micronuclei count can be used as an early indicator for alterations of oral mucosa and exfoliative cytology represents an accessible tool which could be applied for mass screening.

Suggested Citation

  • Anca Maria Pop & Raluca Coroș & Alexandra Mihaela Stoica & Monica Monea, 2021. "Early Diagnosis of Oral Mucosal Alterations in Smokers and E-Cigarette Users Based on Micronuclei Count: A Cross-Sectional Study among Dental Students," IJERPH, MDPI, vol. 18(24), pages 1-10, December.
    • Handle: RePEc:gam:jijerp:v:18:y:2021:i:24:p:13246-:d:703586
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/18/24/13246/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/18/24/13246/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Karolien Adriaens & Dinska Van Gucht & Paul Declerck & Frank Baeyens, 2014. "Effectiveness of the Electronic Cigarette: An Eight-Week Flemish Study with Six-Month Follow-up on Smoking Reduction, Craving and Experienced Benefits and Complaints," IJERPH, MDPI, vol. 11(11), pages 1-29, October.
    2. Silvio Abati & Chiara Bramati & Stefano Bondi & Alessandra Lissoni & Matteo Trimarchi, 2020. "Oral Cancer and Precancer: A Narrative Review on the Relevance of Early Diagnosis," IJERPH, MDPI, vol. 17(24), pages 1-14, December.
    3. Parham Azimi & Zahra Keshavarz & Marianne Lahaie Luna & Jose Guillermo Cedeno Laurent & Jose Vallarino & David C. Christiani & Joseph G. Allen, 2021. "An Unrecognized Hazard in E-Cigarette Vapor: Preliminary Quantification of Methylglyoxal Formation from Propylene Glycol in E-Cigarettes," IJERPH, MDPI, vol. 18(2), pages 1-12, January.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Anca Maria Pop & Raluca Coroș & Alexandra Mihaela Stoica & Monica Monea, 2022. "Reply to Takeshita, W.M.; Ribeiro, D.A. Comment on “Pop et al. Early Diagnosis of Oral Mucosal Alterations in Smokers and E-Cigarette Users Based on Micronuclei Count: A Cross-Sectional Study among De," IJERPH, MDPI, vol. 19(7), pages 1-2, March.
    2. Wilton Mitsunari Takeshita & Daniel Araki Ribeiro, 2022. "Comment on Pop et al. Early Diagnosis of Oral Mucosal Alterations in Smokers and E-Cigarette Users Based on Micronuclei Count: A Cross-Sectional Study among Dental Students. Int. J. Environ. Res. Publ," IJERPH, MDPI, vol. 19(6), pages 1-2, March.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Martijn Van Heel & Dinska Van Gucht & Koen Vanbrabant & Frank Baeyens, 2017. "The Importance of Conditioned Stimuli in Cigarette and E-Cigarette Craving Reduction by E-Cigarettes," IJERPH, MDPI, vol. 14(2), pages 1-18, February.
    2. Oscar Toralla & Pia Lopez Jornet & Eduardo Pons-Fuster, 2022. "The Effect of an Informative Video upon Anxiety and Stress in Patients Requiring an Oral Biopsy: A Randomized Controlled Study," IJERPH, MDPI, vol. 19(2), pages 1-11, January.
    3. Melinda Pénzes & Márta Bakacs & Zoltán Brys & József Vitrai & Gergely Tóth & Zombor Berezvai & Róbert Urbán, 2021. "Vaping-Related Adverse Events and Perceived Health Improvements: A Cross-Sectional Survey among Daily E-Cigarette Users," IJERPH, MDPI, vol. 18(16), pages 1-15, August.
    4. Yuki Taguchi & Shigeaki Toratani & Kensaku Matsui & Seiya Hayashi & Natsuki Eboshida & Atsuko Hamada & Nanako Ito & Fumitaka Obayashi & Naohiro Kimura & Souichi Yanamoto, 2022. "Evaluation of Oral Mucosal Lesions Using the IllumiScan ® Fluorescence Visualisation Device: Distinguishing Squamous Cell Carcinoma," IJERPH, MDPI, vol. 19(16), pages 1-10, August.
    5. Nico Roeschmann & Andrea Rau & Marco R. Kesting & Eva Maier & Mayte Buchbender, 2022. "A Prospective Comparative Analysis Regarding the Assessment of Oral Mucosal Disease Using a Validated Questionnaire to Improve the Teaching of Dental Students," IJERPH, MDPI, vol. 19(15), pages 1-12, August.
    6. Riccardo Polosa & Pasquale Caponnetto & Fabio Cibella & Jacques Le-Houezec, 2015. "Quit and Smoking Reduction Rates in Vape Shop Consumers: A Prospective 12-Month Survey," IJERPH, MDPI, vol. 12(4), pages 1-11, March.
    7. Xiner Wang & Guo Bai & Jizhi Liang & Qianyang Xie & Zhaohan Chen & Erda Zhou & Meng Li & Xiaoling Wei & Liuyang Sun & Zhiyuan Zhang & Chi Yang & Tiger H. Tao & Zhitao Zhou, 2024. "Gustatory interface for operative assessment and taste decoding in patients with tongue cancer," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    8. Roberta Pacifici & Simona Pichini & Silvia Graziano & Manuela Pellegrini & Giuseppina Massaro & Fabio Beatrice, 2015. "Successful Nicotine Intake in Medical Assisted Use of E-Cigarettes: A Pilot Study," IJERPH, MDPI, vol. 12(7), pages 1-9, July.
    9. Karolien Adriaens & Dinska Van Gucht & Frank Baeyens, 2018. "IQOS TM vs. e-Cigarette vs. Tobacco Cigarette: A Direct Comparison of Short-Term Effects after Overnight-Abstinence," IJERPH, MDPI, vol. 15(12), pages 1-20, December.
    10. Dinska Van Gucht & Karolien Adriaens & Frank Baeyens, 2017. "Online Vape Shop Customers Who Use E-Cigarettes Report Abstinence from Smoking and Improved Quality of Life, But a Substantial Minority Still Have Vaping-Related Health Concerns," IJERPH, MDPI, vol. 14(7), pages 1-11, July.
    11. Qian Liu & Yu Liang & Siyi Wang & Zhongguo Huang & Qing Wang & Miaoyutian Jia & Zihang Li & Wai-Kit Ming, 2022. "Health Communication through Chinese Media on E-Cigarette: A Topic Modeling Approach," IJERPH, MDPI, vol. 19(13), pages 1-18, June.
    12. Pietro Ferrara & Saran Shantikumar & Vítor Cabral Veríssimo & Rafael Ruiz-Montero & Cristina Masuet-Aumatell & Josep Maria Ramon-Torrell & the EuroNet MRPH Working Group on Electronic Cigarettes and T, 2019. "Knowledge about E-Cigarettes and Tobacco Harm Reduction among Public Health Residents in Europe," IJERPH, MDPI, vol. 16(12), pages 1-13, June.
    13. repec:eur:ejnmjr:80 is not listed on IDEAS
    14. Anastasia Moysidou & Konstantinos E. Farsalinos & Vassilis Voudris & Kyriakoula Merakou & Kallirrhoe Kourea & Anastasia Barbouni, 2016. "Knowledge and Perceptions about Nicotine, Nicotine Replacement Therapies and Electronic Cigarettes among Healthcare Professionals in Greece," IJERPH, MDPI, vol. 13(5), pages 1-25, May.
    15. Socrates Yong-da Wu & Man Ping Wang & William H. Li & Antonio C. Kwong & Vienna W. Lai & Tai Hing Lam, 2018. "Does Electronic Cigarette Use Predict Abstinence from Conventional Cigarettes among Smokers in Hong Kong?," IJERPH, MDPI, vol. 15(3), pages 1-10, February.
    16. Hsien-Chang Lin & Anne Buu & Wei-Chung Su, 2022. "Disposable E-Cigarettes and Associated Health Risks: An Experimental Study," IJERPH, MDPI, vol. 19(17), pages 1-14, August.
    17. Cristina Russo & Fabio Cibella & Enrico Mondati & Pasquale Caponnetto & Evelise Frazzetto & Massimo Caruso & Grazia Caci & Riccardo Polosa, 2018. "Lack of Substantial Post-Cessation Weight Increase in Electronic Cigarettes Users," IJERPH, MDPI, vol. 15(4), pages 1-13, March.
    18. Shih-Wei Yang & Yun-Shien Lee & Pei-Wen Wu & Liang-Che Chang & Cheng-Cheng Hwang, 2021. "A Retrospective Cohort Study of Oral Leukoplakia in Female Patients—Analysis of Risk Factors Related to Treatment Outcomes," IJERPH, MDPI, vol. 18(16), pages 1-17, August.
    19. repec:eur:ejnmjr:72 is not listed on IDEAS

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:18:y:2021:i:24:p:13246-:d:703586. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.