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Time Length of Negativization and Cycle Threshold Values in 182 Healthcare Workers with Covid-19 in Milan, Italy: An Observational Cohort Study

Author

Listed:
  • Lisa Cariani

    (Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Microbiology Unit, 20122 Milan, Italy
    Contributed equally as first authors.)

  • Beatrice Silvia Orena

    (Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Microbiology Unit, 20122 Milan, Italy
    Specialization School in Microbiology and Virology, University of Milan, 20142 Milan, Italy
    Contributed equally as first authors.)

  • Federico Ambrogi

    (Laboratory of Medical Statistics and Biometry, Giulio A. Maccacaro, Department of Clinical Sciences and Community Health, Campus Cascina Rosa, University of Milan, 20133 Milan, Italy)

  • Simone Gambazza

    (Laboratory of Medical Statistics and Biometry, Giulio A. Maccacaro, Department of Clinical Sciences and Community Health, Campus Cascina Rosa, University of Milan, 20133 Milan, Italy
    UOC Direzione Professioni Sanitarie, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy)

  • Anna Maraschini

    (Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Microbiology Unit, 20122 Milan, Italy)

  • Antonella Dodaro

    (Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Microbiology Unit, 20122 Milan, Italy)

  • Massimo Oggioni

    (Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Virology Unit, 20122 Milan, Italy)

  • Annarosa Orlandi

    (Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Virology Unit, 20122 Milan, Italy)

  • Alessia Pirrone

    (Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Microbiology Unit, 20122 Milan, Italy)

  • Sara Uceda Renteria

    (Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Virology Unit, 20122 Milan, Italy)

  • Mara Bernazzani

    (Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Responsabile Infermieristico area Direzione Medica di Presidio, Coordinamento dipartimenti clinici, Servizio Prevenzione Igiene Ospedaliera, 20122 Milan, Italy)

  • Anna Paola Cantù

    (Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Direzione Medica di Presidio, 20122 Milan, Italy
    Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Romeo ed Enrica Invernizzi Paediatric Research Centre, Department of Biosciences, University of Milan, 20122 Milan, Italy)

  • Ferruccio Ceriotti

    (Fondazione IRCCS Ca’ Granda Ospedale Maggiore, Clinical Laboratory, 20122 Milan, Italy)

  • Giovanna Lunghi

    (Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Virology Unit, 20122 Milan, Italy)

Abstract

Background : Coronavirus Disease 2019 (COVID-19) has rapidly spread worldwide, becoming an unprecedented public health emergency. Rapid detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) suspected cases is crucial to control the spread of infection. We aimed to evaluate the time length of negativization from the onset of symptoms in healthcare workers (HCWs) with COVID-19, and to evaluate significant variations in cycle threshold (CT) values and gene positivity (E, RdRP, and N genes) among positive individuals who returned to work. Methods : We retrospectively analyzed a consecutive cohort of 182 SARS-CoV-2-positive HCWs in Milan, from 16 March to 30 April 2020. Nasopharyngeal swabs were tested by RT-PCR. Results : Asymptomatic HCWs were 17.6% (32/182), and 58 healed at 30 April 2020. The median time length of negativization was 4 weeks (35% of symptomatic versus 40% of asymptomatic HCWs). Four HCWs, healed at 30 April, turned positive within three weeks during controls set up in the work unit. Three-gene positivity had the greatest variability, and increasing CT values from single- to three-gene positivity among all age groups were observed. Conclusions : Self-isolation longer than two weeks and prolonged follow-up periods for the staff returning to work after COVID-19 could be the most suitable choices to counter the SARS-CoV-2 spread. Further studies are needed to investigate infectiousness profiles among positive individuals.

Suggested Citation

  • Lisa Cariani & Beatrice Silvia Orena & Federico Ambrogi & Simone Gambazza & Anna Maraschini & Antonella Dodaro & Massimo Oggioni & Annarosa Orlandi & Alessia Pirrone & Sara Uceda Renteria & Mara Berna, 2020. "Time Length of Negativization and Cycle Threshold Values in 182 Healthcare Workers with Covid-19 in Milan, Italy: An Observational Cohort Study," IJERPH, MDPI, vol. 17(15), pages 1-10, July.
    • Handle: RePEc:gam:jijerp:v:17:y:2020:i:15:p:5313-:d:388713
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    References listed on IDEAS

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    1. Roman Wölfel & Victor M. Corman & Wolfgang Guggemos & Michael Seilmaier & Sabine Zange & Marcel A. Müller & Daniela Niemeyer & Terry C. Jones & Patrick Vollmar & Camilla Rothe & Michael Hoelscher & To, 2020. "Author Correction: Virological assessment of hospitalized patients with COVID-2019," Nature, Nature, vol. 588(7839), pages 35-35, December.
    2. Roman Wölfel & Victor M. Corman & Wolfgang Guggemos & Michael Seilmaier & Sabine Zange & Marcel A. Müller & Daniela Niemeyer & Terry C. Jones & Patrick Vollmar & Camilla Rothe & Michael Hoelscher & To, 2020. "Virological assessment of hospitalized patients with COVID-2019," Nature, Nature, vol. 581(7809), pages 465-469, May.
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