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Decreased Capacity for Sperm Production Induced by Perinatal Bisphenol A Exposure Is Associated with an Increased Inflammatory Response in the Offspring of C57BL/6 Male Mice

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  • Yuan Meng

    (Department of Child and Adolescent Health, School of Public Health, China Medical University, Shenyang 110122, China)

  • Ren Lin

    (Department of Child and Adolescent Health, School of Public Health, China Medical University, Shenyang 110122, China)

  • Fengjuan Wu

    (Department of Child and Adolescent Health, School of Public Health, China Medical University, Shenyang 110122, China)

  • Qi Sun

    (Department of Child and Adolescent Health, School of Public Health, China Medical University, Shenyang 110122, China)

  • Lihong Jia

    (Department of Child and Adolescent Health, School of Public Health, China Medical University, Shenyang 110122, China)

Abstract

Many previous studies have indicated the adverse effects of bisphenol A (BPA) on sperm production and quality; however, the mechanisms underlying BPA male reproductive toxicity have yet to be elucidated. The main purpose of this study was to investigate the effect of perinatal exposure to BPA on the spermatogenic capacity of male offspring, and to explore the possible influence of inflammatory responses in BPA reproductive toxicity. Twenty-one pregnant C57BL/6mice were randomly divided into three groups: a control group, a group receiving 0.2 μg/mL (LBPA), and a group receiving 2 μg/mL of BPA (HBPA), all via drinking water from gestational day 6 to the end of lactation. After weaning, one male mouse was randomly selected from each group ( n = 7/group); these three mice were fed a normal diet and drinking water for 1 month. Levels of serum testosterone (T) and tumor necrosis factor (TNF)-α were then measured in all mice. Sperm count and the proportion of sperm malformation were also determined. The levels of Toll-like receptor 4 (TLR4), nuclear factor (NF)-κB, and aryl hydrocarbon receptor (AhR) protein expression in the testis tissue were determined. Analysis showed that the proportion of sperm malformation increased in the LBPA and HBPA groups ( p < 0.05). Sperm count significantly decreased only in the HBPA group ( p < 0.05), while the levels of serum TNF-α increased in the LBPA and HBPA groups ( p < 0.05). Levels of serum T decreased significantly in the HBPA group, compared with controls ( p < 0.05). Levels of TLR4 and NF-κB protein expression in the testis were significantly higher in the LBPA and HBPA groups ( p < 0.05 or p < 0.01), while AhR protein expression was higher and seminiferous tubules in the testis showed more damage in the HBPA group compared to controls ( p < 0.05 and p < 0.01, respectively). Our results showed that perinatal exposure to low or high doses of BPA decreased the capacity for spermatogenesis in male offspring, which may be associated with an inflammatory response activated by the TLR4/ NF-κB and AhR signaling pathways in the testis.

Suggested Citation

  • Yuan Meng & Ren Lin & Fengjuan Wu & Qi Sun & Lihong Jia, 2018. "Decreased Capacity for Sperm Production Induced by Perinatal Bisphenol A Exposure Is Associated with an Increased Inflammatory Response in the Offspring of C57BL/6 Male Mice," IJERPH, MDPI, vol. 15(10), pages 1-11, October.
  • Handle: RePEc:gam:jijerp:v:15:y:2018:i:10:p:2158-:d:173149
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    References listed on IDEAS

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    1. Cinzia La Rocca & Sabrina Tait & Cristiana Guerranti & Luca Busani & Francesca Ciardo & Bruno Bergamasco & Laura Stecca & Guido Perra & Francesca Romana Mancini & Roberto Marci & Giulia Bordi & Donate, 2014. "Exposure to Endocrine Disrupters and Nuclear Receptor Gene Expression in Infertile and Fertile Women from Different Italian Areas," IJERPH, MDPI, vol. 11(10), pages 1-19, September.
    2. Cinzia La Rocca & Sabrina Tait & Cristiana Guerranti & Luca Busani & Francesca Ciardo & Bruno Bergamasco & Guido Perra & Francesca Romana Mancini & Roberto Marci & Giulia Bordi & Donatella Caserta & S, 2015. "Exposure to Endocrine Disruptors and Nuclear Receptors Gene Expression in Infertile and Fertile Men from Italian Areas with Different Environmental Features," IJERPH, MDPI, vol. 12(10), pages 1-20, October.
    3. George Anifandis & Katerina Katsanaki & Georgia Lagodonti & Christina Messini & Mara Simopoulou & Konstantinos Dafopoulos & Alexandros Daponte, 2018. "The Effect of Glyphosate on Human Sperm Motility and Sperm DNA Fragmentation," IJERPH, MDPI, vol. 15(6), pages 1-8, May.
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