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Bio-Hydrogen Production from Pineapple Waste Extract by Anaerobic Mixed Cultures

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  • Alissara Reungsang

    (Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
    Research Group for Development of Microbial Hydrogen Production Process from Biomass, Khon Kaen University, Khon Kaen 40002, Thailand)

  • Chakkrit Sreela-or

    (Department of Agriculture, Faculty of Food and Agricultural Technology, Phibulsongkram Rajabhat University, Phitsanulok 65000, Thailand)

Abstract

A statistical experimental design was employed to optimize factors that affect the production of hydrogen from the glucose contained in pineapple waste extract by anaerobic mixed cultures. Results from Plackett-Burman design indicated that substrate concentration, initial pH and FeSO 4 concentration had a statistically significant ( p ≤ 0.05) influence on the hydrogen production potential ( P s ) and the specific hydrogen production rate (SHPR). The path of steepest ascent was undertaken to approach the optimal region of these three significant factors which was then optimized using response surface methodology (RSM) with central composite design (CCD). The presence of a substrate concentration of 25.76 g-total sugar/L, initial pH of 5.56, and FeSO 4 concentration of 0.81 g/L gave a maximum predicted P s of 5489 mL H 2 /L, hydrogen yield of 1.83 mol H 2 /mol glucose, and SHPR of 77.31 mL H 2 /g-volatile suspended solid (VSS) h. A verification experiment indicated highly reproducible results with the observed P s and SHPR being only 1.13% and 1.14% different from the predicted values.

Suggested Citation

  • Alissara Reungsang & Chakkrit Sreela-or, 2013. "Bio-Hydrogen Production from Pineapple Waste Extract by Anaerobic Mixed Cultures," Energies, MDPI, vol. 6(4), pages 1-16, April.
    • Handle: RePEc:gam:jeners:v:6:y:2013:i:4:p:2175-2190:d:25088
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    References listed on IDEAS

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    1. Momirlan, M. & Veziroglu, T. N., 2002. "Current status of hydrogen energy," Renewable and Sustainable Energy Reviews, Elsevier, vol. 6(1-2), pages 141-179.
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    Cited by:

    1. Vinayak Laxman Pachapur & Prianka Kutty & Preetika Pachapur & Satinder Kaur Brar & Yann Le Bihan & Rosa Galvez-Cloutier & Gerardo Buelna, 2019. "Seed Pretreatment for Increased Hydrogen Production Using Mixed-Culture Systems with Advantages over Pure-Culture Systems," Energies, MDPI, vol. 12(3), pages 1-26, February.
    2. Asma Sattar & Chaudhry Arslan & Changying Ji & Sumiyya Sattar & Irshad Ali Mari & Haroon Rashid & Fariha Ilyas, 2016. "Comparing the Bio-Hydrogen Production Potential of Pretreated Rice Straw Co-Digested with Seeded Sludge Using an Anaerobic Bioreactor under Mesophilic Thermophilic Conditions," Energies, MDPI, vol. 9(3), pages 1-14, March.
    3. Tran Thi Giang & Siriporn Lunprom & Qiang Liao & Alissara Reungsang & Apilak Salakkam, 2019. "Enhancing Hydrogen Production from Chlorella sp. Biomass by Pre-Hydrolysis with Simultaneous Saccharification and Fermentation (PSSF)," Energies, MDPI, vol. 12(5), pages 1-14, March.

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    pineapple waste extract; bio-hydrogen;

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