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Endomelanconiopsis endophytica Lipase Immobilized in Calcium Alginate for Production of Biodiesel from Waste Cooking Oil

Author

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  • Juliana Gisele Corrêa Rodrigues

    (Programa de Pós-Graduação em Biodiversidade e Biotecnologia, Escola Superior de Ciências da Saúde, Universidade do Estado do Amazonas, Manaus 69050-010, Brazil
    Grupo de Pesquisa Química Aplicada à Tecnologia, Escola Superior de Tecnologia, Universidade do Estado do Amazonas, Manaus 69050-020, Brazil)

  • Fernanda Veras Cardoso

    (Grupo de Pesquisa Química Aplicada à Tecnologia, Escola Superior de Tecnologia, Universidade do Estado do Amazonas, Manaus 69050-020, Brazil)

  • Sergio Duvoisin Junior

    (Grupo de Pesquisa Química Aplicada à Tecnologia, Escola Superior de Tecnologia, Universidade do Estado do Amazonas, Manaus 69050-020, Brazil)

  • Nélio Teixeira Machado

    (Faculdade de Engenharia Sanitária e Ambiental, Universidade Federal do Pará, Belém 66075-910, Brazil)

  • Patrícia Melchionna Albuquerque

    (Programa de Pós-Graduação em Biodiversidade e Biotecnologia, Escola Superior de Ciências da Saúde, Universidade do Estado do Amazonas, Manaus 69050-010, Brazil
    Grupo de Pesquisa Química Aplicada à Tecnologia, Escola Superior de Tecnologia, Universidade do Estado do Amazonas, Manaus 69050-020, Brazil)

Abstract

The increasing global demand for biodiesel is due to the urgent need to replace fossil diesel with a fuel based on renewable energy sources. Although chemical catalysis is widely used to produce biodiesel, it uses harsh operating conditions, has high energy consumption, and generates unwanted byproducts. In this scenario, biocatalysis stands out as an efficient and environmentally friendly alternative to chemical catalysis. In biocatalysis, the use of immobilized enzymes plays an important role in the reduction in costs. In this sense, we investigated the use of the lipase produced by an Amazonian endophytic fungus in an immobilized form in the transesterification of waste cooking oil for biodiesel production. The fungus Endomelanconiopsis endophytica QAT_7AC demonstrated a high production of lipase. The lipolytic extract was precipitated in ethanol, which increased the specific enzyme activity. The lipolytic extract and the precipitated lipolytic extract were immobilized in calcium alginate beads. Immobilization efficiency was over 89%. The immobilized biocatalysts showed thermal stability and were used in the production of biodiesel using waste cooking oil and ethanol. It was possible to reuse them for up to four reaction cycles, with yields greater than 70%. These results prove the efficiency of immobilized biocatalysts in the production of biodiesel from waste oils.

Suggested Citation

  • Juliana Gisele Corrêa Rodrigues & Fernanda Veras Cardoso & Sergio Duvoisin Junior & Nélio Teixeira Machado & Patrícia Melchionna Albuquerque, 2024. "Endomelanconiopsis endophytica Lipase Immobilized in Calcium Alginate for Production of Biodiesel from Waste Cooking Oil," Energies, MDPI, vol. 17(22), pages 1-16, November.
    • Handle: RePEc:gam:jeners:v:17:y:2024:i:22:p:5520-:d:1513949
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    References listed on IDEAS

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    1. Chattopadhyay, Soham & Sen, Ramkrishna, 2013. "Fuel properties, engine performance and environmental benefits of biodiesel produced by a green process," Applied Energy, Elsevier, vol. 105(C), pages 319-326.
    2. Atadashi, I.M. & Aroua, M.K. & Aziz, A. Abdul, 2011. "Biodiesel separation and purification: A review," Renewable Energy, Elsevier, vol. 36(2), pages 437-443.
    3. Juliana Gisele Corrêa Rodrigues & Fernanda Veras Cardoso & Celine Campos dos Santos & Rosiane Rodrigues Matias & Nélio Teixeira Machado & Sergio Duvoisin Junior & Patrícia Melchionna Albuquerque, 2023. "Biocatalyzed Transesterification of Waste Cooking Oil for Biodiesel Production Using Lipase from the Amazonian Fungus Endomelanconiopsis endophytica," Energies, MDPI, vol. 16(19), pages 1-19, October.
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