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Saccharification Yield through Enzymatic Hydrolysis of the Steam-Exploded Pinewood

Author

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  • Merve Nazli Borand

    (Energy Systems Engineering Department, Faculty of Engineering, Merkez Campus, Yalova University, 77100 Yalova, Turkey)

  • Asli Isler Kaya

    (Chemical Engineering Department, Faculty of Chemical and Metallurgical Engineering, Ayazaga Campus Istanbul Technical University, 34467 Istanbul, Turkey)

  • Filiz Karaosmanoglu

    (Chemical Engineering Department, Faculty of Chemical and Metallurgical Engineering, Ayazaga Campus Istanbul Technical University, 34467 Istanbul, Turkey)

Abstract

Pressure, temperature, and retention time are the most studied parameters in steam explosion pretreatment. However, this work aimed to fix these parameters and to evaluate the influences of several less investigated steam explosion parameters on the saccharification yield in hydrolysis. In this study, firstly, pinewood samples smaller than 200 µm were treated with steam explosion at 190 °C for 10 min. The variable parameters were biomass loading, N 2 pressure, and release time. Steam-exploded samples were hydrolyzed with the Trichoderma reesei enzyme for saccharification for 72 h. The sugar content of the resultant products was analyzed to estimate the yield of sugars (such as glucose, xylose, galactose, mannose, and arabinose). The best glucose yield in the pulp was achieved with 4 g of sample, N 2 pressure of 0.44 MPa, and short release time (22 s). These conditions gave a glucose yield of 97.72% in the pulp, and the xylose, mannose, galactose, and arabinose yields in the liquid fraction were found to be 85.59%, 87.76%, 86.43%, and 90.3%, respectively.

Suggested Citation

  • Merve Nazli Borand & Asli Isler Kaya & Filiz Karaosmanoglu, 2020. "Saccharification Yield through Enzymatic Hydrolysis of the Steam-Exploded Pinewood," Energies, MDPI, vol. 13(17), pages 1-12, September.
  • Handle: RePEc:gam:jeners:v:13:y:2020:i:17:p:4552-:d:407955
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    References listed on IDEAS

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    5. Raud, M. & Krennhuber, K. & Jäger, A. & Kikas, T., 2019. "Nitrogen explosive decompression pre-treatment: An alternative to steam explosion," Energy, Elsevier, vol. 177(C), pages 175-182.
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    1. Isler-Kaya, Asli & Karaosmanoglu, Filiz, 2022. "Life cycle assessment of safflower and sugar beet molasses-based biofuels," Renewable Energy, Elsevier, vol. 201(P1), pages 1127-1138.

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