IDEAS home Printed from https://ideas.repec.org/a/gam/jeners/v12y2019i23p4495-d290960.html
   My bibliography  Save this article

Batch and Fed-Batch Ethanol Fermentation of Cheese-Whey Powder with Mixed Cultures of Different Yeasts

Author

Listed:
  • Csilla Farkas

    (Research Centre for Bioengineering and Process Engineering, Faculty of Food Science, Szent István University, Ménesi út 45, H-1118 Budapest, Hungary)

  • Judit M. Rezessy-Szabó

    (Research Centre for Bioengineering and Process Engineering, Faculty of Food Science, Szent István University, Ménesi út 45, H-1118 Budapest, Hungary)

  • Vijai Kumar Gupta

    (Department of Chemistry and Biotechnology, ERA Chair of Green Chemistry, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia
    ERA Chair-VALORTECH, Estonian University of Life Sciences, Kreutzwaldi 56/5, 51014 Tartu, Estonia)

  • Erika Bujna

    (Research Centre for Bioengineering and Process Engineering, Faculty of Food Science, Szent István University, Ménesi út 45, H-1118 Budapest, Hungary)

  • Tuan M. Pham

    (Research Centre for Bioengineering and Process Engineering, Faculty of Food Science, Szent István University, Ménesi út 45, H-1118 Budapest, Hungary)

  • Klára Pásztor-Huszár

    (Research Centre for Food Technology, Faculty of Food Science, Szent István University, Ménesi út 45, H-1118 Budapest, Hungary)

  • László Friedrich

    (Research Centre for Food Technology, Faculty of Food Science, Szent István University, Ménesi út 45, H-1118 Budapest, Hungary)

  • Rajeev Bhat

    (ERA Chair-VALORTECH, Estonian University of Life Sciences, Kreutzwaldi 56/5, 51014 Tartu, Estonia)

  • Vijay Kumar Thakur

    (Enhanced Composites and Structures Center, School of Aerospace, Transport and Manufacturing, Cranfield University, Bedfordshire MK43 0AL, UK)

  • Quang D. Nguyen

    (Research Centre for Bioengineering and Process Engineering, Faculty of Food Science, Szent István University, Ménesi út 45, H-1118 Budapest, Hungary)

Abstract

Eight yeast strains of Lachancea thermotolerans , Kluyveromyces marxianus , and Kluyveromyces waltii have been tested for their ability to ferment lactose into ethanol in mashes containing 10% ( w / v ) cheese whey powder (CWP). The K. marxianus NCAIM Y00963 achieved 3.5% ( v / v ) ethanol concentration at 96–120 h of fermentation. The ethanol production by the selected lactose-positive strains and the well-known ethanologenic Saccharomyces cerevisiae (Levuline Fb) in mixed culture was also investigated at different CWP concentrations and inoculation techniques in batch mode. The mixed culture in an equal ratio (1:1) of cell counts of K. marxianus and S. serevisiae showed an increase in lactose conversion rate. The two yeast strains in a ratio of 3:1 (three-quarters of K. marxianus and a quarter of S. cerevisiae in a total of 4.5 × 10 10 cells) resulted in 72.33% efficiency of lactose bioconversion and 7.6% ( v / v ) ethanol production at 17.5% ( w / v ) of CWP concentration. In the repeated inoculation process, with the addition of three-quarter part of 3:1 ratio of mixed culture (3.3 × 10 10 cells of K. marxianus ) into 150 mL CWP mash at initiation and the rest quarter part (1.2 × 10 10 cells of S. cerevisiae ) at 24 h, 8.86% ( v / v ) ethanol content with 87.5% efficiency of lactose conversion was reached. Both the ethanol concentration and efficiency of bioconversion were increased to 10.34% ( v / v ) and 92%, respectively, by combination with fed-batch fermentation technology. Our results can serve a very good basis for the development of industrial technology for the utilization of cheese whey.

Suggested Citation

  • Csilla Farkas & Judit M. Rezessy-Szabó & Vijai Kumar Gupta & Erika Bujna & Tuan M. Pham & Klára Pásztor-Huszár & László Friedrich & Rajeev Bhat & Vijay Kumar Thakur & Quang D. Nguyen, 2019. "Batch and Fed-Batch Ethanol Fermentation of Cheese-Whey Powder with Mixed Cultures of Different Yeasts," Energies, MDPI, vol. 12(23), pages 1-12, November.
  • Handle: RePEc:gam:jeners:v:12:y:2019:i:23:p:4495-:d:290960
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1996-1073/12/23/4495/pdf
    Download Restriction: no

    File URL: https://www.mdpi.com/1996-1073/12/23/4495/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Ling, K. Charles, 2008. "Whey to Ethanol: A Biofuel Role for Dairy Cooperatives?," Research Reports 280102, United States Department of Agriculture, Rural Development.
    2. Aspasia A. Chatzipaschali & Anastassios G. Stamatis, 2012. "Biotechnological Utilization with a Focus on Anaerobic Treatment of Cheese Whey: Current Status and Prospects," Energies, MDPI, vol. 5(9), pages 1-34, September.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Abdulkhani, Ali & Alizadeh, Peyman & Hedjazi, Sahab & Hamzeh, Yahya, 2017. "Potential of Soya as a raw material for a whole crop biorefinery," Renewable and Sustainable Energy Reviews, Elsevier, vol. 75(C), pages 1269-1280.
    2. Aspasia A. Chatzipaschali & Anastassios G. Stamatis, 2012. "Biotechnological Utilization with a Focus on Anaerobic Treatment of Cheese Whey: Current Status and Prospects," Energies, MDPI, vol. 5(9), pages 1-34, September.
    3. Ling, K. Charles, 2011. "Cooperative Theory, Practice, and Financing: A Dairy Cooperative Case Study," Research Reports 280109, United States Department of Agriculture, Rural Development.
    4. Esmanur Sirmacekic & Atilgan Atilgan & Roman Rolbiecki & Barbara Jagosz & Stanisław Rolbiecki & Osman Gokdogan & Marcin Niemiec & Joanna Kocięcka, 2022. "Possibilities of Using Whey Wastes in Agriculture: Case of Turkey," Energies, MDPI, vol. 15(24), pages 1-17, December.
    5. Stefano Papirio & Silvio Matassa & Francesco Pirozzi & Giovanni Esposito, 2020. "Anaerobic Co-Digestion of Cheese Whey and Industrial Hemp Residues Opens New Perspectives for the Valorization of Agri-Food Waste," Energies, MDPI, vol. 13(11), pages 1-13, June.
    6. Margarita Andreas Dareioti & Aikaterini Ioannis Vavouraki & Konstantina Tsigkou & Michael Kornaros, 2021. "Assessment of Single- vs. Two-Stage Process for the Anaerobic Digestion of Liquid Cow Manure and Cheese Whey," Energies, MDPI, vol. 14(17), pages 1-14, August.
    7. Charalambous, Panagiotis & Shin, Juhee & Shin, Seung Gu & Vyrides, Ioannis, 2020. "Anaerobic digestion of industrial dairy wastewater and cheese whey: Performance of internal circulation bioreactor and laboratory batch test at pH 5-6," Renewable Energy, Elsevier, vol. 147(P1), pages 1-10.
    8. Gupta, Anubhuti & Verma, Jay Prakash, 2015. "Sustainable bio-ethanol production from agro-residues: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 41(C), pages 550-567.
    9. Hegde, Swati & Lodge, Jeffery S. & Trabold, Thomas A., 2018. "Characteristics of food processing wastes and their use in sustainable alcohol production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 81(P1), pages 510-523.
    10. Miguel Casallas-Ojeda & Luz Elba Torres-Guevara & Diana M. Caicedo-Concha & María F. Gómez, 2021. "Opportunities for Waste to Energy in the Milk Production Industry: Perspectives for the Circular Economy," Sustainability, MDPI, vol. 13(22), pages 1-24, November.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jeners:v:12:y:2019:i:23:p:4495-:d:290960. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.