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Direct Conversion of Minimally Pretreated Corncob by Enzyme-Intensified Microbial Consortia

Author

Listed:
  • Alei Geng

    (Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China)

  • Nana Li

    (Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China)

  • Anaiza Zayas-Garriga

    (Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China)

  • Rongrong Xie

    (Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China)

  • Daochen Zhu

    (Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China)

  • Jianzhong Sun

    (Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China)

Abstract

The presence of diverse carbohydrate-active enzymes (CAZymes) is crucial for the direct bioconversion of lignocellulose. In this study, various anaerobic microbial consortia were employed for the degradation of 10 g/L of minimally pretreated corncob. The involvement of lactic acid bacteria (LAB) and a CAZyme-rich bacterium ( Bacteroides cellulosilyticus or Paenibacillus lautus ) significantly enhanced the lactic acid production by Ruminiclostridium cellulolyticum from 0.74 to 2.67 g/L ( p < 0.01), with a polysaccharide conversion of 67.6%. The supplement of a commercial cellulase cocktail, CTec 2, into the microbial consortia continuously promoted the lactic acid production to up to 3.35 g/L, with a polysaccharide conversion of 80.6%. Enzymatic assays, scanning electron microscopy, and Fourier transform infrared spectroscopy revealed the substantial functions of these CAZyme-rich consortia in partially increasing enzyme activities, altering the surface structure of biomass, and facilitating substrate decomposition. These results suggested that CAZyme-intensified consortia could significantly improve the levels of bioconversion of lignocellulose. Our work might shed new light on the construction of intensified microbial consortia for direct conversion of lignocellulose.

Suggested Citation

  • Alei Geng & Nana Li & Anaiza Zayas-Garriga & Rongrong Xie & Daochen Zhu & Jianzhong Sun, 2024. "Direct Conversion of Minimally Pretreated Corncob by Enzyme-Intensified Microbial Consortia," Agriculture, MDPI, vol. 14(9), pages 1-13, September.
    • Handle: RePEc:gam:jagris:v:14:y:2024:i:9:p:1610-:d:1478241
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    References listed on IDEAS

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    1. Didier Ndeh & Artur Rogowski & Alan Cartmell & Ana S. Luis & Arnaud Baslé & Joseph Gray & Immacolata Venditto & Jonathon Briggs & Xiaoyang Zhang & Aurore Labourel & Nicolas Terrapon & Fanny Buffetto &, 2017. "Correction: Corrigendum: Complex pectin metabolism by gut bacteria reveals novel catalytic functions," Nature, Nature, vol. 548(7669), pages 612-612, August.
    2. Didier Ndeh & Artur Rogowski & Alan Cartmell & Ana S. Luis & Arnaud Baslé & Joseph Gray & Immacolata Venditto & Jonathon Briggs & Xiaoyang Zhang & Aurore Labourel & Nicolas Terrapon & Fanny Buffetto &, 2017. "Complex pectin metabolism by gut bacteria reveals novel catalytic functions," Nature, Nature, vol. 544(7648), pages 65-70, April.
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