IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v544y2017i7648d10.1038_nature21725.html
   My bibliography  Save this article

Complex pectin metabolism by gut bacteria reveals novel catalytic functions

Author

Listed:
  • Didier Ndeh

    (Institute for Cell and Molecular Biosciences, Newcastle University)

  • Artur Rogowski

    (Institute for Cell and Molecular Biosciences, Newcastle University
    †Present addresses: Megazyme, Bray, Co. Wicklow, A98 YV29, Ireland (A.R.); Institute for Wine Biotechnology, Department of Viticulture and Oenology, Stellenbosch University, Matieland 7602, South Africa (F.B.).)

  • Alan Cartmell

    (Institute for Cell and Molecular Biosciences, Newcastle University)

  • Ana S. Luis

    (Institute for Cell and Molecular Biosciences, Newcastle University)

  • Arnaud Baslé

    (Institute for Cell and Molecular Biosciences, Newcastle University)

  • Joseph Gray

    (Institute for Cell and Molecular Biosciences, Newcastle University)

  • Immacolata Venditto

    (Institute for Cell and Molecular Biosciences, Newcastle University)

  • Jonathon Briggs

    (Institute for Cell and Molecular Biosciences, Newcastle University)

  • Xiaoyang Zhang

    (Institute for Cell and Molecular Biosciences, Newcastle University)

  • Aurore Labourel

    (Institute for Cell and Molecular Biosciences, Newcastle University)

  • Nicolas Terrapon

    (Architecture et Fonction des Macromolécules Biologiques, Centre National de la Recherche Scientifique (CNRS), Aix-Marseille University)

  • Fanny Buffetto

    (INRA, UR1268 Biopolymères Interactions Assemblages
    †Present addresses: Megazyme, Bray, Co. Wicklow, A98 YV29, Ireland (A.R.); Institute for Wine Biotechnology, Department of Viticulture and Oenology, Stellenbosch University, Matieland 7602, South Africa (F.B.).)

  • Sergey Nepogodiev

    (John Innes Centre Norwich Research Park)

  • Yao Xiao

    (University of Michigan Medical School)

  • Robert A. Field

    (John Innes Centre Norwich Research Park)

  • Yanping Zhu

    (Complex Carbohydrate Research Center, The University of Georgia)

  • Malcolm A. O’Neill

    (Complex Carbohydrate Research Center, The University of Georgia)

  • Breeanna R. Urbanowicz

    (Complex Carbohydrate Research Center, The University of Georgia)

  • William S. York

    (Complex Carbohydrate Research Center, The University of Georgia)

  • Gideon J. Davies

    (University of York)

  • D. Wade Abbott

    (Lethbridge Research Centre)

  • Marie-Christine Ralet

    (INRA, UR1268 Biopolymères Interactions Assemblages)

  • Eric C. Martens

    (University of Michigan Medical School)

  • Bernard Henrissat

    (Architecture et Fonction des Macromolécules Biologiques, Centre National de la Recherche Scientifique (CNRS), Aix-Marseille University
    INRA, USC 1408 AFMB
    King Abdulaziz University)

  • Harry J. Gilbert

    (Institute for Cell and Molecular Biosciences, Newcastle University)

Abstract

The metabolism of carbohydrate polymers drives microbial diversity in the human gut microbiota. It is unclear, however, whether bacterial consortia or single organisms are required to depolymerize highly complex glycans. Here we show that the gut bacterium Bacteroides thetaiotaomicron uses the most structurally complex glycan known: the plant pectic polysaccharide rhamnogalacturonan-II, cleaving all but 1 of its 21 distinct glycosidic linkages. The deconstruction of rhamnogalacturonan-II side chains and backbone are coordinated to overcome steric constraints, and the degradation involves previously undiscovered enzyme families and catalytic activities. The degradation system informs revision of the current structural model of rhamnogalacturonan-II and highlights how individual gut bacteria orchestrate manifold enzymes to metabolize the most challenging glycan in the human diet.

Suggested Citation

  • Didier Ndeh & Artur Rogowski & Alan Cartmell & Ana S. Luis & Arnaud Baslé & Joseph Gray & Immacolata Venditto & Jonathon Briggs & Xiaoyang Zhang & Aurore Labourel & Nicolas Terrapon & Fanny Buffetto &, 2017. "Complex pectin metabolism by gut bacteria reveals novel catalytic functions," Nature, Nature, vol. 544(7648), pages 65-70, April.
    • Handle: RePEc:nat:nature:v:544:y:2017:i:7648:d:10.1038_nature21725
    DOI: 10.1038/nature21725
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature21725
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature21725?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Erika C. Freeman & Erik J. S. Emilson & Thorsten Dittmar & Lucas P. P. Braga & Caroline E. Emilson & Tobias Goldhammer & Christine Martineau & Gabriel Singer & Andrew J. Tanentzap, 2024. "Universal microbial reworking of dissolved organic matter along environmental gradients," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. María Ángeles Rivas & Rocío Casquete & Alberto Martín & María de Guía Córdoba & Emilio Aranda & María José Benito, 2021. "Strategies to Increase the Biological and Biotechnological Value of Polysaccharides from Agricultural Waste for Application in Healthy Nutrition," IJERPH, MDPI, vol. 18(11), pages 1-19, June.
    3. Yonggan Sun & Qixing Nie & Shanshan Zhang & Huijun He & Sheng Zuo & Chunhua Chen & Jingrui Yang & Haihong Chen & Jielun Hu & Song Li & Jiaobo Cheng & Baojie Zhang & Zhitian Zheng & Shijie Pan & Ping H, 2023. "Parabacteroides distasonis ameliorates insulin resistance via activation of intestinal GPR109a," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    4. Alei Geng & Nana Li & Anaiza Zayas-Garriga & Rongrong Xie & Daochen Zhu & Jianzhong Sun, 2024. "Direct Conversion of Minimally Pretreated Corncob by Enzyme-Intensified Microbial Consortia," Agriculture, MDPI, vol. 14(9), pages 1-13, September.
    5. Hao-Tian Wang & Zi-Long Wang & Kuan Chen & Ming-Ju Yao & Meng Zhang & Rong-Shen Wang & Jia-He Zhang & Hans Ågren & Fu-Dong Li & Junhao Li & Xue Qiao & Min Ye, 2023. "Insights into the missing apiosylation step in flavonoid apiosides biosynthesis of Leguminosae plants," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    6. Stefan Dyksma & Michael Pester, 2023. "Oxygen respiration and polysaccharide degradation by a sulfate-reducing acidobacterium," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    7. Diego E. Sastre & Nazneen Sultana & Marcos V. A. S. Navarro & Maros Huliciak & Jonathan Du & Javier O. Cifuente & Maria Flowers & Xu Liu & Pete Lollar & Beatriz Trastoy & Marcelo E. Guerin & Eric J. S, 2024. "Human gut microbes express functionally distinct endoglycosidases to metabolize the same N-glycan substrate," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:544:y:2017:i:7648:d:10.1038_nature21725. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.