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Transcriptomic Analysis Reveals the Mechanism of MtLOX24 in Response to Methyl Jasmonate Stress in Medicago truncatula

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  • Lei Xu

    (State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China
    Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
    These authors contributed equally to this work.)

  • Yanchao Xu

    (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
    These authors contributed equally to this work.)

  • Huanhuan Lv

    (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

  • Yanran Xu

    (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

  • Jiangqi Wen

    (Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK 73401, USA)

  • Mingna Li

    (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

  • Junmei Kang

    (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

  • Zhipeng Liu

    (State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China)

  • Qingchuan Yang

    (State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China
    Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

  • Ruicai Long

    (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

Abstract

Lipoxygenase (LOX) is associated with responses to plant hormones, environmental stresses, and signaling substances. Methyl jasmonate (MeJA) treatment triggers the production of LOX, polyphenol oxidase, and protease inhibitors in various plants, producing resistance to herbivory. To examine the response of MtLOX24 to MeJA, the phenotypic and physiological changes in Medicago truncatula MtLOX24 overexpression and lox mutant plants were investigated. Additionally, wild-type R108, the MtLOX24 -overexpressing line L4, and the mutant lox-1 were utilized as experimental materials to characterize the differentially expressed genes (DEGs) and metabolic pathways in response to MeJA. The results indicate that after treatment with 200 µM of MeJA, the damage in the mutants lox-1 and lox-2 was more serious than in the overexpressing lines L4 and L6, with more significant leaf wilting, yellowing, and oxidative damage in lox-1 and lox-2 . Exogenous application of MeJA induced H 2 O 2 production and POD activity but reduced CAT activity in the lox mutants. Transcriptome analysis revealed 10,238 DEGs in six libraries of normal-growing groups (cR108, cL4, and clox1) and MeJA-treated groups (R108, L4, and lox1). GO and KEGG functional enrichment analysis demonstrated that under normal growth conditions, the DEGs between the cL4 vs. cR108 and the clox-1 vs. cR108 groups were primarily enriched in signaling pathways such as plant–pathogen interactions, flavonoid biosynthesis, plant hormone signal transduction, the MAPK signaling pathway, and glutathione metabolism. The DEGs of the R108 vs. cR108 and L4 vs. cL4 groups after MeJA treatment were mainly enriched in glutathione metabolism, phenylpropanoid biosynthesis, the MAPK signaling pathway, circadian rhythm, and α-linolenic acid metabolism. Among them, under normal growth conditions, genes like PTI5, PR1, HSPs, PALs, CAD, CCoAOMT, and CYPs showed significant differences between L4 and the wild type, suggesting that the expression of these genes is impacted by MtLOX24 overexpression. CDPKs, CaMCMLs, IFS, JAZ, and other genes were also significantly different between L4 and the wild type upon MeJA treatment, suggesting that they might be important genes involved in JA signaling. This study provides a reference for the study of the response mechanism of MtLOX24 under MeJA signaling.

Suggested Citation

  • Lei Xu & Yanchao Xu & Huanhuan Lv & Yanran Xu & Jiangqi Wen & Mingna Li & Junmei Kang & Zhipeng Liu & Qingchuan Yang & Ruicai Long, 2024. "Transcriptomic Analysis Reveals the Mechanism of MtLOX24 in Response to Methyl Jasmonate Stress in Medicago truncatula," Agriculture, MDPI, vol. 14(7), pages 1-20, July.
  • Handle: RePEc:gam:jagris:v:14:y:2024:i:7:p:1076-:d:1428685
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    References listed on IDEAS

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    1. Hehong Zhang & Fengmin Wang & Weiqi Song & Zihang Yang & Lulu Li & Qiang Ma & Xiaoxiang Tan & Zhongyan Wei & Yanjun Li & Junmin Li & Fei Yan & Jianping Chen & Zongtao Sun, 2023. "Different viral effectors suppress hormone-mediated antiviral immunity of rice coordinated by OsNPR1," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    2. Jeffery L. Dangl & Jonathan D. G. Jones, 2001. "Plant pathogens and integrated defence responses to infection," Nature, Nature, vol. 411(6839), pages 826-833, June.
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