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Effects of Nitrogen Addition on Soil Microbial Functional Diversity and Extracellular Enzyme Activities in Greenhouse Cucumber Cultivation

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  • Zhen Wang

    (College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
    Key Laboratory of Protected Horticulture of the Education Ministry and Liaoning Province, Shenyang 110866, China
    National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China
    Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866, China)

  • Shuang Wang

    (College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
    Key Laboratory of Protected Horticulture of the Education Ministry and Liaoning Province, Shenyang 110866, China
    National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China
    Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866, China)

  • Ting Bian

    (College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
    Key Laboratory of Protected Horticulture of the Education Ministry and Liaoning Province, Shenyang 110866, China
    National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China
    Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866, China)

  • Qiaobo Song

    (College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
    Key Laboratory of Protected Horticulture of the Education Ministry and Liaoning Province, Shenyang 110866, China
    National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China
    Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866, China)

  • Guorui Wu

    (College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
    Key Laboratory of Protected Horticulture of the Education Ministry and Liaoning Province, Shenyang 110866, China
    National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China
    Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866, China)

  • Muhammad Awais

    (College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
    Key Laboratory of Protected Horticulture of the Education Ministry and Liaoning Province, Shenyang 110866, China
    National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China
    Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866, China)

  • Yufeng Liu

    (College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
    Key Laboratory of Protected Horticulture of the Education Ministry and Liaoning Province, Shenyang 110866, China
    National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China
    Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866, China)

  • Hongdan Fu

    (College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
    Key Laboratory of Protected Horticulture of the Education Ministry and Liaoning Province, Shenyang 110866, China
    National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China
    Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866, China)

  • Zhouping Sun

    (College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
    Key Laboratory of Protected Horticulture of the Education Ministry and Liaoning Province, Shenyang 110866, China
    National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China
    Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866, China)

Abstract

Greenhouses, commonly used for vegetable production, are experiencing large nitrogen (N) inputs in North China, which leads to soil acidification, increases soil N availability, and affects microbial community structure and composition. However, it remains unclear how N enrichment influences soil microbial functional activities in this region. In this study, we conducted a two-year pot experiment in a greenhouse to evaluate the effects of four different rates of N addition (0, 334, 668, and 1002 kg N ha −1 year −1 ) on cucumber soil properties, extracellular enzyme activities, and community level physiological profiles (CLPP). We found that high-N addition (1002 kg N ha −1 ) caused a massive accumulation of inorganic nitrogen and soil acidification, which was not beneficial to soil microbial activities. The color development (AWCD) values for the metabolism of microbial carbon sources and the activities of soil extracellular enzymes also showed a significant decrease in high N(N3) treatment. Additionally, the activity of leucine aminopeptidase (LAP) and polyphenol oxidase (PPO) of N3 decreased by 36% and 50% compared to the N0 and could be a good predictor for microbial functional diversity and microbial biomass carbon (MBC). Structural equation modeling (SEM) confirmed that the reduction of microbial functional diversity is mainly coregulated by the decline of soil pH and the change of cucumber BGB (belowground biomass) resulting from soil C and N imbalance. Overall, excessive N-fertilizer amendment can be more dangerous to microbial community functional diversity, especially for carbohydrate utilization which adversely affects cucumber yield in current intensive management.

Suggested Citation

  • Zhen Wang & Shuang Wang & Ting Bian & Qiaobo Song & Guorui Wu & Muhammad Awais & Yufeng Liu & Hongdan Fu & Zhouping Sun, 2022. "Effects of Nitrogen Addition on Soil Microbial Functional Diversity and Extracellular Enzyme Activities in Greenhouse Cucumber Cultivation," Agriculture, MDPI, vol. 12(9), pages 1-15, September.
    • Handle: RePEc:gam:jagris:v:12:y:2022:i:9:p:1366-:d:904555
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    References listed on IDEAS

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    1. Nicolas Gruber & James N. Galloway, 2008. "An Earth-system perspective of the global nitrogen cycle," Nature, Nature, vol. 451(7176), pages 293-296, January.
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