IDEAS home Printed from https://ideas.repec.org/a/gam/jagris/v12y2022i3p333-d758271.html
   My bibliography  Save this article

Characterization of the Endophytic Mycobiome in Cowpea ( Vigna unguiculata ) from a Single Location Using Illumina Sequencing

Author

Listed:
  • Tonjock Rosemary Kinge

    (Department of Genetics, University of the Free State, Bloemfontein 9310, South Africa
    Department of Biological Sciences, University of Bamenda, Bambili P.O. Box 39, Cameroon)

  • Soumya Ghosh

    (Department of Genetics, University of the Free State, Bloemfontein 9310, South Africa)

  • Errol D. Cason

    (Department of Animal Sciences, University of the Free State, Bloemfontein 9301, South Africa)

  • Marieka Gryzenhout

    (Department of Genetics, University of the Free State, Bloemfontein 9310, South Africa)

Abstract

Cowpea is an important crop for small-scale farmers in poor areas but is also being developed for commercial agriculture as a possible substitute for commercial legumes. Endophytic fungi are omnipresent and play crucial but diverse roles in plants. This study characterized the endophyte component of the cowpea mycobiome from leaves, main and crown stems and roots using Illumina MiSeq of the ITS2 region of the ribosomal operon. Ascomycetes exhibited the highest diversity, with Molecular Operational Taxonomic Units (MOTUs) assigned as Macrophomina , Cladosporium , Phoma , Fusarium and Cryptococcus , among the most dominant genera. Certain MOTUS showed preferential colonization patterns for above or below ground tissues. Several MOTU generic groups known to include phytopathogenic species were found, with relative abundances ranging from high to very low. Phylogenetic analyses of reads for some MOTUs showed that a level of identification could be obtained to species level. It also confirmed the absences of other species, including phytopathogens. This is the first study that adopted a holistic metagenomic typing approach to study the fungal endophytes of cowpea from a single location, a crop that is so integral for low-income households of the world.

Suggested Citation

  • Tonjock Rosemary Kinge & Soumya Ghosh & Errol D. Cason & Marieka Gryzenhout, 2022. "Characterization of the Endophytic Mycobiome in Cowpea ( Vigna unguiculata ) from a Single Location Using Illumina Sequencing," Agriculture, MDPI, vol. 12(3), pages 1-15, February.
    • Handle: RePEc:gam:jagris:v:12:y:2022:i:3:p:333-:d:758271
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2077-0472/12/3/333/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2077-0472/12/3/333/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Derek S. Lundberg & Sarah L. Lebeis & Sur Herrera Paredes & Scott Yourstone & Jase Gehring & Stephanie Malfatti & Julien Tremblay & Anna Engelbrektson & Victor Kunin & Tijana Glavina del Rio & Robert , 2012. "Defining the core Arabidopsis thaliana root microbiome," Nature, Nature, vol. 488(7409), pages 86-90, August.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Giuseppe Malgioglio & Giulio Flavio Rizzo & Sebastian Nigro & Vincent Lefebvre du Prey & Joelle Herforth-Rahmé & Vittoria Catara & Ferdinando Branca, 2022. "Plant-Microbe Interaction in Sustainable Agriculture: The Factors That May Influence the Efficacy of PGPM Application," Sustainability, MDPI, vol. 14(4), pages 1-28, February.
    2. Jake M. Robinson & Jacob G. Mills & Martin F. Breed, 2018. "Walking Ecosystems in Microbiome-Inspired Green Infrastructure: An Ecological Perspective on Enhancing Personal and Planetary Health," Challenges, MDPI, vol. 9(2), pages 1-15, November.
    3. Luigi Russi & Gianpiero Marconi & Nicoletta Ferradini & Beatrice Farda & Marika Pellegrini & Loretta Pace, 2022. "Investigating Population Genetic Diversity and Rhizosphere Microbiota of Central Apennines’ Artemisia eriantha," Sustainability, MDPI, vol. 14(18), pages 1-14, September.
    4. Amrita Gupta & Udai B. Singh & Pramod K. Sahu & Surinder Paul & Adarsh Kumar & Deepti Malviya & Shailendra Singh & Pandiyan Kuppusamy & Prakash Singh & Diby Paul & Jai P. Rai & Harsh V. Singh & Madhab, 2022. "Linking Soil Microbial Diversity to Modern Agriculture Practices: A Review," IJERPH, MDPI, vol. 19(5), pages 1-29, March.
    5. Ying Wang & Mei Wang & Zhen’an Yang & Yalin Jiao & Guangming Chu, 2022. "Dominant Fungal Communities Aggregate in the Shallow Rhizosphere Soil of Anabasis aphylla," Sustainability, MDPI, vol. 14(22), pages 1-14, November.
    6. Jianli Liao & Jun Ye & Yun Liang & Muhammad Khalid & Danfeng Huang, 2019. "Pakchoi Antioxidant Improvement and Differential Rhizobacterial Community Composition under Organic Fertilization," Sustainability, MDPI, vol. 11(8), pages 1-16, April.
    7. Carla L. Abán & Giovanni Larama & Antonella Ducci & Jorgelina Huidobro & Michel Abanto & Silvina Vargas-Gil & Carolina Pérez-Brandan, 2022. "Soil Properties and Bacterial Communities Associated with the Rhizosphere of the Common Bean after Using Brachiaria brizantha as a Service Crop: A 10-Year Field Experiment," Sustainability, MDPI, vol. 15(1), pages 1-23, December.
    8. Nicholas Ozede Igiehon & Olubukola Oluranti Babalola, 2018. "Rhizosphere Microbiome Modulators: Contributions of Nitrogen Fixing Bacteria towards Sustainable Agriculture," IJERPH, MDPI, vol. 15(4), pages 1-25, March.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jagris:v:12:y:2022:i:3:p:333-:d:758271. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.