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Extraction, Identification and Characterization by Sds-Page Method of Collagen Extracted from Rhizostoma Pulmo, a Jellyfish Found in the Black Sea Basin

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  • Ana-Maria Pesterau

    (University of Medicine and and Pharmacy Carol Davila, Bucharest, Romania)

  • Sirbu Rodica
  • Emin Cadar

Abstract

Collagen is one of the proteins that plays an important role in many biomedical and cosmetic applications. The present study investigates collagen extracted from the jellyfish Rhizostoma pulmo, a species native to the Black Sea basin. The method of extracting collagen from the marine organism is described. The SDS-PAGE (Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis) method, an efficient technique for the separation and analysis of proteins according to molecular weight, was used to identify and characterize the protein structure of the extracted collagen. The collagen samples obtained were purified and subjected to electrophoresis, demonstrating a specific band corresponding to type I collagen, the main type of collagen found in marine organisms. The results show a protein structure similar to that of collagen isolated from other marine sources, indicating the high potential of Rhizostoma pulmo as a source of collagen for industrial applications. This research contributes to the development of sustainable methods for the valorization of marine resources from the Black Sea basin in fields such as regenerative, tissue, dermato-cosmetic, nutraceutical and even food medicine.

Suggested Citation

  • Ana-Maria Pesterau & Sirbu Rodica & Emin Cadar, 2024. "Extraction, Identification and Characterization by Sds-Page Method of Collagen Extracted from Rhizostoma Pulmo, a Jellyfish Found in the Black Sea Basin," European Journal of Natural Sciences and Medicine, European Center for Science Education and Research, vol. 7, December.
    • Handle: RePEc:eur:ejnmjr:80
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    References listed on IDEAS

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    1. Silvio Abati & Chiara Bramati & Stefano Bondi & Alessandra Lissoni & Matteo Trimarchi, 2020. "Oral Cancer and Precancer: A Narrative Review on the Relevance of Early Diagnosis," IJERPH, MDPI, vol. 17(24), pages 1-14, December.
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