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Towards a practical industrial 2G ethanol production process based on immobilized recombinant S. cerevisiae: Medium and strain selection for robust integrated fixed-bed reactor operation

Author

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  • Perez, Caroline L.
  • Pereira, Laís P.R. da C.
  • Milessi, Thais S.
  • Sandri, Juliana P.
  • Demeke, Mekonnen
  • Foulquié-Moreno, Maria R.
  • Thevelein, Johan M.
  • Zangirolami, Teresa C.

Abstract

The development of a robust and scalable process for 2G ethanol production from renewable feedstock requires high ethanol titers and long-term stability. A strategy based on strain evaluation and integration with 1G ethanol production was used to overcome these challenges. Three engineered superior S. cerevisiae strains were evaluated in industrial media, using either pure sugarcane bagasse hydrolysates or mixed with molasses from sugarcane processing. Cell recycling was limited to 4 batches and decreasing productivity was observed in fermentation media based on concentrated bagasse hydrolysate, even after detoxification. When crude bagasse hydrolysate was mixed with molasses (MBH medium), fermentations with up to 10 cell recycles and final ethanol concentrations over 50 g/L were achieved with all strains. In a fixed-bed reactor operated with the selected MDS130 strain and MBH medium, remarkable ethanol productivities up to 22.8 g/L/h were reached and up to 20 cell recycles were possible.

Suggested Citation

  • Perez, Caroline L. & Pereira, Laís P.R. da C. & Milessi, Thais S. & Sandri, Juliana P. & Demeke, Mekonnen & Foulquié-Moreno, Maria R. & Thevelein, Johan M. & Zangirolami, Teresa C., 2022. "Towards a practical industrial 2G ethanol production process based on immobilized recombinant S. cerevisiae: Medium and strain selection for robust integrated fixed-bed reactor operation," Renewable Energy, Elsevier, vol. 185(C), pages 363-375.
    • Handle: RePEc:eee:renene:v:185:y:2022:i:c:p:363-375
    DOI: 10.1016/j.renene.2021.12.044
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    References listed on IDEAS

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    1. Singh, Anita & Sharma, Punita & Saran, Alok Kumar & Singh, Namita & Bishnoi, Narsi R., 2013. "Comparative study on ethanol production from pretreated sugarcane bagasse using immobilized Saccharomyces cerevisiae on various matrices," Renewable Energy, Elsevier, vol. 50(C), pages 488-493.
    2. Mishra, Abhishek & Sharma, Ajay K. & Sharma, Sumit & Bagai, Rashmi & Mathur, Anshu S. & Gupta, Ravi P. & Tuli, Deepak K., 2016. "Lignocellulosic ethanol production employing immobilized Saccharomyces cerevisiae in packed bed reactor," Renewable Energy, Elsevier, vol. 98(C), pages 57-63.
    3. Gutiérrez-Rivera, Beatriz & Ortiz-Muñiz, Beningo & Gómez-Rodríguez, Javier & Cárdenas-Cágal, Angel & Domínguez González, José Manuel & Aguilar-Uscanga, Maria Guadalupe, 2015. "Bioethanol production from hydrolyzed sugarcane bagasse supplemented with molasses “B” in a mixed yeast culture," Renewable Energy, Elsevier, vol. 74(C), pages 399-405.
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    1. Mondal, Sourav & Neogi, Swati & Chakraborty, Saikat, 2024. "Optimization of reactor parameters for amplifying synergy in enzymatic co-hydrolysis and microbial co-fermentation of lignocellulosic agro-residues," Renewable Energy, Elsevier, vol. 225(C).

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