IDEAS home Printed from https://ideas.repec.org/a/caa/jnlvet/v54y2009i6id54-2009-vetmed.html
   My bibliography  Save this article

Distribution of Mycobacterium avium subsp.paratuberculosis in tissues of naturally infected cattle as affected by age

Author

Listed:
  • L. Hasonova

    (Veterinary Research Institute, Brno, Czech Republic
    Faculty of Agriculture, University of South Bohemia, Ceske Budejovice, Czech Republic)

  • I. Trcka

    (Veterinary Research Institute, Brno, Czech Republic)

  • V. Babak

    (Veterinary Research Institute, Brno, Czech Republic)

  • Z. Rozsypalova

    (Veterinary Research Institute, Brno, Czech Republic)

  • R. Pribylova

    (Veterinary Research Institute, Brno, Czech Republic)

  • I. Pavlik

    (Veterinary Research Institute, Brno, Czech Republic)

Abstract

ABSTRACT: The objectives of this study were to investigate Mycobacterium avium subsp. paratuberculosis (MAP) distribution in various tissues, in faeces and in milk samples from 131 animals originating from one imported Jersey cattle herd from Denmark. MAP was detected by culture in 37.4% animals. Massive MAP growth was most often observed in the small intestines (48 animals). The lowest levels of MAP were found in spleen and mammary gland samples. MAP was detected in the faeces of 8.4% animals; however, milk samples were MAP negative by culture. The highest prevalence of MAP infection (42.9%) was in age Group B (1.6 to 3 years) and the lowest (6.1%) in group D (more than 8 years). Seven positive calves were detected in the youngest age group; one of them was less than one month of age, which could imply an intrauterine infection. MAP shedding in faeces by a five-month-old calf was also confirmed. The IS900 RFLP (restriction fragment length polymorphism) type B-C1 was identified in all animals.

Suggested Citation

  • L. Hasonova & I. Trcka & V. Babak & Z. Rozsypalova & R. Pribylova & I. Pavlik, 2009. "Distribution of Mycobacterium avium subsp.paratuberculosis in tissues of naturally infected cattle as affected by age," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 54(6), pages 257-269.
  • Handle: RePEc:caa:jnlvet:v:54:y:2009:i:6:id:54-2009-vetmed
    DOI: 10.17221/54/2009-VETMED
    as

    Download full text from publisher

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/54/2009-VETMED.html
    Download Restriction: free of charge

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/54/2009-VETMED.pdf
    Download Restriction: free of charge

    File URL: https://libkey.io/10.17221/54/2009-VETMED?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. W.y Ayele & M. Macháčková & I. Pavlík, 2001. "The transmission and impact of paratuberculosis infection in domestic and wild ruminants," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 46(7-8), pages 205-224.
    2. T. Amemori & L. Matlova & O. A Fischer & W. Y Ayele & M. Machackova & E. Gopfert & I. Pavlik, 2004. "Distribution of Mycobacterium avium subsp. paratuberculosis in the gastrointestinal tract of shedding cows and its application to laparoscopic biopsy," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 49(7), pages 225-236.
    3. I. Slana & F. Paolicchi & B. Janstova & P. Navratilova & I. Pavlik, 2008. "Detection methods for Mycobacterium avium subsp paratuberculosis in milk and milk products: a review," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 53(6), pages 283-306.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. M. Kopecna & I. Parmova & L. Dvorska-Bartosova & M. Moravkova & V. Babak & I. Pavlik, 2008. "Distribution and transmission of Mycobacterium avium subspecies paratuberculosis in farmed red deer (Cervus elaphus) studied by faecal culture, serology and IS900 RFLP examinations," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 53(9), pages 510-523.
    2. V. Beran & M. Havelkova & J. Kaustova & L. Dvorska & I. Pavlik, 2006. "Cell wall deficient forms of mycobacteria: a review," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(7), pages 365-389.
    3. J.L. Khol & V. Beran & P. Kralik & M. Trckova & I. Pavlik & W. Baumgartner, 2010. "Grass silage contaminated with Mycobacterium avium subspecies paratuberculosis (MAP): a possible source of paratuberculosis infection in ruminants?," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 55(5), pages 225-232.
    4. M. Moravkova & I. Trcka & J. Lamka & I. Pavlik, 2008. "A mixed infection of Mycobacterium avium subsp. paratuberculosis and M. a. hominissuis in one red deer (Cervus elaphus) studied by IS900 BstEII and IS1245 PvuII RFLP analyses: a case report," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 53(8), pages 445-451.
    5. M. Toman & M. Faldyna & I. Pavlik, 2003. "Immunological characteristics of cattle with Mycobacterium avium subsp. paratuberculosis infection," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 48(6), pages 147-154.
    6. M. Kaevska & K. Hruska, 2010. "Mycobacteria in water, feedstocks and food: analysis of publications," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 55(12), pages 571-580.
    7. K. Hruska & M. Bartos & P. Kralik & I. Pavlik, 2005. "Mycobacterium avium subsp. paratuberculosis in powdered infant milk: paratuberculosis in cattle - the public health problem to be solved," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 50(8), pages 327-335.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlvet:v:54:y:2009:i:6:id:54-2009-vetmed. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.