IDEAS home Printed from https://ideas.repec.org/a/caa/jnlvet/v49y2004i7id5699-vetmed.html
   My bibliography  Save this article

Distribution of Mycobacterium avium subsp. paratuberculosis in the gastrointestinal tract of shedding cows and its application to laparoscopic biopsy

Author

Listed:
  • T. Amemori

    (Veterinary Research Institute, Brno, Czech Republic)

  • L. Matlova

    (Veterinary Research Institute, Brno, Czech Republic)

  • O. A Fischer

    (Veterinary Research Institute, Brno, Czech Republic)

  • W. Y Ayele

    (Veterinary Research Institute, Brno, Czech Republic)

  • M. Machackova

    (Veterinary Research Institute, Brno, Czech Republic)

  • E. Gopfert

    (Veterinary Research Institute, Brno, Czech Republic)

  • I. Pavlik

    (Veterinary Research Institute, Brno, Czech Republic)

Abstract

The gastrointestinal tract (GIT) is a major target for Mycobacterium avium subsp. paratuberculosis (M. a. paratuberculosis) in cattle. Culture examination was achieved in tissue samples obtained from 10 different regions of the GIT (proximal and distal parts of the duodenum, proximal, middle and distal parts of the jejunum, proximal and distal parts of the ileum, the ileocecal valve, the caecum and the rectum) and their adjacent lymph nodes. The culture results were statistically analysed to elucidate the distribution of M. a. paratuberculosis in the GIT. A total of 63 cows older than 24 months were diagnosed with paratuberculosis by faecal and tissue cultures. The better detection rate of M. a. paratuberculosis was found in the mucosae from the jejunum to the ileocecal valve and in the lymph nodes from the jejunum to the caecum. The mean number of colony forming units (CFU) in the mucosae and the lymph nodes of the distal jejunum and the proximal ileum was significantly higher than that in the mucosae of the duodenum, the caecum and the rectum, and in the lymph nodes of the duodenum and the rectum, respectively (p < 0.05). Laparoscopic biopsy attempted out on 4 animals to test its potential use for sample collection from the statistically optimal mesenteric lymph nodes; but resulted in an abortive attempt because these targets were encircled by the intestines, the pressure of which complicated the laparoscopic approach.

Suggested Citation

  • T. Amemori & L. Matlova & O. A Fischer & W. Y Ayele & M. Machackova & E. Gopfert & I. Pavlik, 2004. "Distribution of Mycobacterium avium subsp. paratuberculosis in the gastrointestinal tract of shedding cows and its application to laparoscopic biopsy," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 49(7), pages 225-236.
    • Handle: RePEc:caa:jnlvet:v:49:y:2004:i:7:id:5699-vetmed
    DOI: 10.17221/5699-VETMED
    as

    Download full text from publisher

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5699-VETMED.html
    Download Restriction: free of charge
    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5699-VETMED.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/5699-VETMED?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. P. Svastova & I. Pavlik & M. Bartos, 2002. "Rapid differentiation of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. paratuberculosis by amplification of insertion element IS901," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 47(5), pages 117-121.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. M. Kopecna & I. Parmova & L. Dvorska-Bartosova & M. Moravkova & V. Babak & I. Pavlik, 2008. "Distribution and transmission of Mycobacterium avium subspecies paratuberculosis in farmed red deer (Cervus elaphus) studied by faecal culture, serology and IS900 RFLP examinations," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 53(9), pages 510-523.
    2. L. Hasonova & I. Trcka & V. Babak & Z. Rozsypalova & R. Pribylova & I. Pavlik, 2009. "Distribution of Mycobacterium avium subsp.paratuberculosis in tissues of naturally infected cattle as affected by age," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 54(6), pages 257-269.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. I. Pavlik & P. Jahn & L. Dvorska & M. Bartos & L. Novotny & R. Halouzka, 2004. "Mycobacterial infections in horses: a review of the literature," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 49(11), pages 427-440.
    2. J. Hosek & P. Svastova & M. Moravkova & I. Pavlik & M. Bartos, 2006. "Methods of mycobacterial DNA isolation from different biological material: a review," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(5), pages 180-192.
    3. J.E. Shitaye & R. Halouzka & J. Svobodova & V. Grymova & M. Grym & M. Skoric & P. Fictum & V. Beran & M. Slany & I. Pavlik, 2010. "First isolation of Mycobacterium genavense in a blue headed parrot (Pionus menstruus) imported from Surinam (South America) to the Czech Republic: a case report," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 55(7), pages 339-347.
    4. K. Hruska, 2004. "Research on paratuberculosis: Analysis of publications 1994-2004," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 49(8), pages 271-282.

    More about this item

    Keywords

    Johne's disease; diagnostic; IS900; PCR;
    All these keywords.

    JEL classification:

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlvet:v:49:y:2004:i:7:id:5699-vetmed. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.