IDEAS home Printed from https://ideas.repec.org/a/caa/jnlvet/v53y2008i8id1927-vetmed.html
   My bibliography  Save this article

A mixed infection of Mycobacterium avium subsp. paratuberculosis and M. a. hominissuis in one red deer (Cervus elaphus) studied by IS900 BstEII and IS1245 PvuII RFLP analyses: a case report

Author

Listed:
  • M. Moravkova

    (Veterinary Research Institute, Brno, Czech Republic)

  • I. Trcka

    (Veterinary Research Institute, Brno, Czech Republic)

  • J. Lamka

    (Faculty of Pharmacy, Charles University, Hradec Kralove, Czech Republic)

  • I. Pavlik

    (Veterinary Research Institute, Brno, Czech Republic)

Abstract

A mixed infection with Mycobacterium avium subsp. paratuberculosis (MAP) and Mycobacterium avium subsp. hominissuis (MAH) in one naturally infected red deer stag from a game park is described. The animal was euthanized because of symptoms of poor condition, weight loss and chronic diarrhoea. In spite of that, pathological lesions were observed only in the mesenteric lymph nodes, which were five to ten times enlarged with confluent caseous granulomas of 1 to 10 mm in size. Mycobacteria were isolated from all studied samples: a mixed infection of MAP and MAH was confirmed by multiplex PCR for the detection of IS 900, IS9011, IS1245 and dnaJ. MAP of the identical IS900 BstEII RFLP type C1 was isolated from all tissue samples and faeces. MAH isolates were detected in six examined tissue samples, including three mesenteric lymph nodes with caseous granulomas. Only minor differences in the band numbers and position of four different IS1245 PvuII RFLP patterns of MAH isolates were found. It follows from these results that red deer may potentially be infected with MAH, when a MAP infection is under way.

Suggested Citation

  • M. Moravkova & I. Trcka & J. Lamka & I. Pavlik, 2008. "A mixed infection of Mycobacterium avium subsp. paratuberculosis and M. a. hominissuis in one red deer (Cervus elaphus) studied by IS900 BstEII and IS1245 PvuII RFLP analyses: a case report," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 53(8), pages 445-451.
    • Handle: RePEc:caa:jnlvet:v:53:y:2008:i:8:id:1927-vetmed
    DOI: 10.17221/1927-VETMED
    as

    Download full text from publisher

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1927-VETMED.html
    Download Restriction: free of charge
    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1927-VETMED.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/1927-VETMED?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. W.y Ayele & M. Macháčková & I. Pavlík, 2001. "The transmission and impact of paratuberculosis infection in domestic and wild ruminants," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 46(7-8), pages 205-224.
    2. I. Pavlik & L. Matlova & L. Dvorska & J.E. Shitaye & I. Parmova, 2005. "Mycobacterial infections in cattle and pigs caused by Mycobacterium aviumcomplex members and atypical mycobacteria in theCzech Republicduring 2000-2004," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 50(7), pages 281-290.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. M. Blahutkova & P. Fictum & M. Skoric & B. Bezdekova & P. Jahn & P. Kriz & V. Mrlik & I. Slana & M. Kaevska & I. Pavlik, 2011. "Mycobacterium avium subsp. hominissuis infection in two sibling Fjord horses diagnosed using quantitative real time PCR: a case report," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 56(6), pages 294-301.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. I. Pavlik & I. Trcka & I. Parmova & J. Svobodova & I. Melicharek & G. Nagy & Z. Cvetnic & M. Ocepek & M. Pate & M. Lipiec, 2005. "Detection of bovine and human tuberculosis in cattle and other animals in six Central European countries during the years 2000-2004," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 50(7), pages 291-299.
    2. Petr Králík & Hana Přikrylová Vondrušková & Iva Slaná & Monika Morávková & Ivo Pavlík, 2011. "Survival of Mycobacterium avium subsp. hominissuis in homemade smoked pork sausages," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 29(6), pages 647-653.
    3. V. Beran & M. Havelkova & J. Kaustova & L. Dvorska & I. Pavlik, 2006. "Cell wall deficient forms of mycobacteria: a review," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(7), pages 365-389.
    4. J.L. Khol & V. Beran & P. Kralik & M. Trckova & I. Pavlik & W. Baumgartner, 2010. "Grass silage contaminated with Mycobacterium avium subspecies paratuberculosis (MAP): a possible source of paratuberculosis infection in ruminants?," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 55(5), pages 225-232.
    5. M. Toman & M. Faldyna & I. Pavlik, 2003. "Immunological characteristics of cattle with Mycobacterium avium subsp. paratuberculosis infection," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 48(6), pages 147-154.
    6. L. Hasonova & I. Trcka & V. Babak & Z. Rozsypalova & R. Pribylova & I. Pavlik, 2009. "Distribution of Mycobacterium avium subsp.paratuberculosis in tissues of naturally infected cattle as affected by age," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 54(6), pages 257-269.
    7. M. Pate & M. Moravkova & B. Krt & I. Pavlik & M. Ocepek, 2009. "Genotyping of Mycobacterium avium subsp. avium isolates from domestic animals in Slovenia by IS901 RFLP," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 54(6), pages 270-279.
    8. K. Hruska & M. Bartos & P. Kralik & I. Pavlik, 2005. "Mycobacterium avium subsp. paratuberculosis in powdered infant milk: paratuberculosis in cattle - the public health problem to be solved," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 50(8), pages 327-335.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlvet:v:53:y:2008:i:8:id:1927-vetmed. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.