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Modeling the Structure of RNA Molecules with Small-Angle X-Ray Scattering Data

Author

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  • Michal Jan Gajda
  • Denise Martinez Zapien
  • Emiko Uchikawa
  • Anne-Catherine Dock-Bregeon

Abstract

We propose a novel fragment assembly method for low-resolution modeling of RNA and show how it may be used along with small-angle X-ray solution scattering (SAXS) data to model low-resolution structures of particles having as many as 12 independent secondary structure elements. We assessed this model-building procedure by using both artificial data on a previously proposed benchmark and publicly available data. With the artificial data, SAXS-guided models show better similarity to native structures than ROSETTA decoys. The publicly available data showed that SAXS-guided models can be used to reinterpret RNA structures previously deposited in the Protein Data Bank. Our approach allows for fast and efficient building of de novo models of RNA using approximate secondary structures that can be readily obtained from existing bioinformatic approaches. We also offer a rigorous assessment of the resolving power of SAXS in the case of small RNA structures, along with a small multimetric benchmark of the proposed method.

Suggested Citation

  • Michal Jan Gajda & Denise Martinez Zapien & Emiko Uchikawa & Anne-Catherine Dock-Bregeon, 2013. "Modeling the Structure of RNA Molecules with Small-Angle X-Ray Scattering Data," PLOS ONE, Public Library of Science, vol. 8(11), pages 1-14, November.
  • Handle: RePEc:plo:pone00:0078007
    DOI: 10.1371/journal.pone.0078007
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    References listed on IDEAS

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    1. Andrew G. Fraser & Ravi S. Kamath & Peder Zipperlen & Maruxa Martinez-Campos & Marc Sohrmann & Julie Ahringer, 2000. "Functional genomic analysis of C. elegans chromosome I by systematic RNA interference," Nature, Nature, vol. 408(6810), pages 325-330, November.
    2. Daisuke Sakakibara & Atsuko Sasaki & Teppei Ikeya & Junpei Hamatsu & Tomomi Hanashima & Masaki Mishima & Masatoshi Yoshimasu & Nobuhiro Hayashi & Tsutomu Mikawa & Markus Wälchli & Brian O. Smith & Mas, 2009. "Protein structure determination in living cells by in-cell NMR spectroscopy," Nature, Nature, vol. 458(7234), pages 102-105, March.
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